胰岛素样生长因子1对糖尿病大鼠伴骨折模型骨折愈合的影响

发布时间：2018-04-30 19:43

本文选题：胰岛素生长因子 + 糖尿病　；参考：《中国老年学杂志》2017年18期

【摘要】：目的探讨胰岛素生长因子(IGF)-1对糖尿病(DM)模型大鼠骨折后骨折愈合的影响及其机制。方法选取健康成年雄性SD大鼠40只,对所有大鼠进行编号后采用随机数字表法选取10只作为正常组、其余30只大鼠采用腹腔注射链脲佐菌素建造糖尿病大鼠模型,建模成功后采用随机数字表法分为IGF-1组、胰岛素组、模型组各10只,各组大鼠均建立左胫骨上段骨折模型,分别给予对应的处理措施,分别于骨折后第1、2、4、6周观察四组大鼠的骨折愈合情况、骨痂组织中相关指标的变化差异、血清骨钙素等指标的含量差异。结果各组大鼠第1、2、4、6周血清骨钙素含量差异显著(P0.05),模型组显著低于正常组、IGF-1组和胰岛素组(P0.05),正常组、IGF-1组和胰岛素组间比较无统计学差异(P0.05)。各组大鼠第1、2、4、6周血钙、血清骨形态发生蛋白(BMP)-2、碱性磷酸酶(ALP)含量差异显著(P0.05),模型组显著低于正常组、IGF-1组和胰岛素组(P0.05),在第1、2周时IGF-1组和胰岛素组血钙值、血清BMP-2、ALP水平明显低于正常组(P0.05),在第4、6周无显著差异(P0.05)。结论IGF-1对DM模型大鼠骨折具有促进骨折愈合的作用,其机制可能与提高血清骨钙素、BMP-2、ALP及血钙水平有关。
[Abstract]:Objective to investigate the effect and mechanism of insulin-growth factor (IGF-1) on fracture healing in rats with diabetes mellitus (DM). Methods A total of 40 healthy adult male SD rats were selected. 10 rats were selected as normal group by random number table method, and the other 30 rats were treated by intraperitoneal injection of streptozotocin (STZ) to construct diabetic rat model. After modeling successfully, the rats were randomly divided into IGF-1 group, insulin group and model group, 10 rats in each group. The model of left upper tibia fracture was established in each group, and the corresponding treatment measures were given respectively. The healing of fracture, the change of relative indexes in callus and the content of serum osteocalcin were observed at the 1st and 2nd week after fracture in the rats of the four groups. Results the level of serum osteocalcin was significantly lower in the model group than that in the normal group (P 0.05) and insulin group (P 0.05). There was no significant difference between the normal group and the insulin group (P 0.05). The levels of serum calcium, bone morphogenetic protein (BMPP) and alkaline phosphatase (ALP) were significantly lower in the model group than in the normal group (P 0.05). The serum calcium levels in the IGF-1 group and the insulin group were significantly lower than those in the normal control group and the insulin group at the 1st week. The level of serum BMP-2ALP was significantly lower than that of the normal group (P0.05A), and there was no significant difference in the level of BMP-2ALP at the 4th week (P0.05). Conclusion IGF-1 can promote fracture healing in DM model rats, and its mechanism may be related to the increase of serum osteocalcin, BMP-2, ALP and serum calcium levels.
【作者单位】：湖北医药学院附属襄阳医院;
【分类号】：R-332;R587.1;R683

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