室内燃煤PM2.5对卵蛋白诱导哮喘大鼠的气道炎症作用

发布时间：2018-04-30 20:39

本文选题：燃煤PM. + OVA　；参考：《实用医学杂志》2017年05期

【摘要】：目的:探讨室内燃煤PM2.5对卵蛋白(OVA)诱导哮喘大鼠的气道炎症作用及其对肺组织病理改变。方法:将40只6周龄雄性SD大鼠随机分为Control、OVA、PM2.5、PM2.5+OVA 4组,通过气管滴注分别予以生理盐水、OVA(15μg/mL)和(或)PM2.5(2.5 mg/mL)混悬液200μL,2周/次,共4次。末次染毒24 h后收集支气管肺泡灌洗液(Balf)检测炎性细胞的分布、白介素4(IL-4)、γ干扰素(IFN-γ),右上肺组织做HE染色及电镜切片。结果:PM2.5+OVA组炎性细胞较Control组增多(P0.05),主要以淋巴细胞及单核细胞为主;HE染色镜下见Control组炎性细胞浸润较少,PM2.5组及OVA组炎性细胞中等量浸润,PM2.5+OVA组大量炎性细胞浸润;电镜下Control组未见异常,OVA组可见有大量即将脱落细胞,局部可见II型细胞破损,PM2.5组可见内皮细胞广泛水肿,肺泡间隔可见大量纤维,PM2.5+OVA组炎性细胞浸润,细胞器大量破坏,基底膜厚度不均,气血屏障结构不清晰;PM2.5+OVA组IL-4与Control组、OVA组及PM2.5组间均有差异(P0.05),IFN-γPM2.5+OVA组较Control组有差异(P0.05),IL-4与IFN-γ呈负相关(r=-0.358,P0.05)。结论:室内燃煤PM2.5加重OVA诱导的哮喘大鼠气道炎性细胞浸润及病理性改变。
[Abstract]:Aim: to investigate the airway inflammation and the pathological changes of lung tissue in asthmatic rats induced by ovalbumin ovalbumin (OVA). Methods: forty 6-week-old male Sprague-Dawley rats were randomly divided into 4 groups: control OVA PM2.5 渭 g / mL OVA group. The rats were treated with 15 渭 g / mL of normal saline and (or 2.5 mg / mL) of PM2.5mg / mL suspension for 2 weeks for 4 times. The distribution of inflammatory cells was detected in bronchoalveolar lavage fluid (BALF) 24 h after the last exposure. IL-4, IFN- 纬, HE staining and electron microscopy were used in the right upper lung tissue. Results the number of inflammatory cells in OVA group was higher than that in Control group (P 0.05). The infiltration of inflammatory cells in Control group was less than that in PM2.5 group and OVA group. Under HE staining, a large number of inflammatory cells were infiltrated in PM2.5 OVA group. Under electron microscope, there were a large number of exfoliated cells in Control group, extensive edema of endothelial cells in PM2.5 group, inflammatory cell infiltration in alveolar septum and destruction of organelle in PM2.5 group. The thickness of basement membrane was uneven and the structure of blood barrier was not clear. There was significant difference between IL-4 and Control group (P 0.05) and PM2.5 group (P 0.05). There was a negative correlation between IL-4 and IFN- 纬 in P0.05- 纬 PM2.5 OVA group and IFN- 纬. Conclusion: Indoor coal burning PM2.5 can aggravate the infiltration and pathological changes of airway inflammatory cells induced by OVA in asthmatic rats.
【作者单位】：遵义医学院公共卫生学院;思南县人民医院病理科;
【基金】：国家自然科学基金(编号:81360419、81560527) 贵州省科技厅社会发展攻关项目(编号:SY字(2013)3027、SY字(2012)3126)
【分类号】：R562.25

【相似文献】