循环miRNA-93在DHEA多囊卵巢综合征大鼠模型中的表达及其意义

发布时间：2018-05-01 05:07

本文选题：多囊卵巢综合征(PCOS) + 脱氢表雄酮(DHEA)　；参考：《现代妇产科进展》2017年12期

【摘要】：目的:通过检测多囊卵巢综合征(PCOS)大鼠血清与卵巢组织中miRNA-93及血管内皮生长因子A(VEGFA)的表达水平,探讨循环miRNA-93及靶基因VEGFA在PCOS发生发展过程中的作用机理与诊断价值。方法:选取3~4周龄雌性(SD)大鼠,随机分为实验组和对照组,每组20只。实验组每日颈背部皮下注射脱氢表雄酮(DHEA),按6mg/100g剂量溶于0.2ml无菌注射用油;对照组每日颈背部皮下注射0.2ml无菌注射用油剂。28天后观察两组大鼠的动情周期、卵巢形态学、性激素、体重、卵巢体积、OGTT血糖、空腹胰岛素、胰岛素抵抗指数(HOMA-IR)及血脂等代谢相关指标的变化。RT-PCR法检测大鼠血清和卵巢组织中miRNA-93表达,ELISA法检测血清中VEGFA表达,IHC、Western blot法分析卵巢组织中VEGFA蛋白表达量。结果:实验组大鼠失去规律的动情周期,卵巢体积增大,呈多囊样变,血清FSH、T、LH升高,糖耐量异常,空腹胰岛素及胰岛素抵抗指数明显高于对照组,血清胆固醇、甘油三酯高于对照组,PCOS大鼠模型构建成功。RT-PCR、ELISA、Western blot、IHC等结果显示,miRNA-93及靶基因VEGFA在PCOS大鼠模型的血清、卵巢组织中表达一致且显著升高(P0.05)。结论:miRNA-93在PCOS大鼠模型血清和卵巢组织中呈高表达,可能通过调控其靶基因VEGFA在PCOS发生发展中起重要作用,在PCOS诊断中循环miRNA-93可能作为一种新型非侵入性的诊断标记物。
[Abstract]:Objective: to investigate the mechanism and diagnostic value of circulating miRNA-93 and target gene VEGFA in the pathogenesis and development of polycystic ovary syndrome (PCOS) by detecting the expression of miRNA-93 and VEGF in serum and ovary of rats. Methods: three weeks old female SD rats were randomly divided into experimental group and control group with 20 rats in each group. The experimental group was injected with dehydroepiandrosterone (DHEAA) subcutaneously daily and dissolved in 0.2ml aseptic injection oil according to the dose of 6mg/100g. The oestrous cycle, ovary morphology and sex hormone were observed in the control group after subcutaneous injection of 0.2ml aseptic injection oil for the control group for 28 days. Body weight, ovarian volume, OGTT blood glucose, fasting insulin, Changes of insulin resistance index (HOMA-IRI) and serum lipids. RT-PCR was used to detect the expression of miRNA-93 in serum and ovarian tissues. Elisa was used to detect the expression of VEGFA in serum. Results: the rats in the experimental group lost the regular estrous cycle, increased the volume of ovary, presented polycystic degeneration, increased serum FSHT, LH, impaired glucose tolerance, and the fasting insulin and insulin resistance index were significantly higher than those in the control group. The results showed that the expression of miRNA-93 and target gene VEGFA in serum of PCOS rat model was consistent and significantly increased in ovarian tissue. Conclusion the high expression of 10 miRNA-93 in serum and ovarian tissue of PCOS rat model may play an important role in the pathogenesis and development of PCOS by regulating its target gene VEGFA. Circulating miRNA-93 may be a novel noninvasive diagnostic marker in the diagnosis of PCOS.
【作者单位】：河南科技大学临床医学院河南科技大学第一附属医院;
【基金】：河南省卫计委科技攻关项目(No:201503140)
【分类号】：R-332;R711.75

【相似文献】