血清视黄醇结合蛋白-4与2型糖尿病及肥胖的相关性研究

发布时间：2018-05-01 11:28

本文选题：视黄醇结合蛋白-4 + 2型糖尿病　；参考：《苏州大学》2008年硕士论文

【摘要】： [目的]研究不同糖耐量及体重人群中血清视黄醇结合蛋白-4(RBP4)水平的变化,并分析其与其它相关代谢和临床生化指标的关系,从而进一步探讨RBP4这个新近发现的又一脂肪因子与胰岛素抵抗之间的相关性,以及其在2型糖尿病及肥胖发生发展中可能所起的作用。 [方法]采用酶联免疫吸附法检测13例正常糖调节正常体重者(NW-NGR)、14例正常糖调节超重/肥胖者(OW/OB-NGR)、26例2型糖尿病正常体重者(NW-T2DM)及34例2型糖尿病超重/肥胖者(OW/OB-T2DM)空腹状态下血清视黄醇结合蛋白-4(RBP4)的水平;同时测定所有受试者的空腹血浆真胰岛素(FINS)、空腹血糖(FBG)、糖化血红蛋白(HBA1C)、总胆固醇(TC)、甘油三脂(TG)、高密度脂蛋白胆固醇(HDL-C)、低密度脂蛋白胆固醇(LDL-C)、收缩压(SBP)、舒张压(DBP)、身高、体重、腰围(W)、臀围(H),并计算腰臀比(WHR)、体重指数(BMI);以稳态模型评估法计算胰岛素抵抗指数(HOMA-IR):HOMA-IR=FBG×FINS/22.5。应用SPSS11.5for windows统计学软件分析不同糖调节及体重人群血清RBP4水平的变化以及血清RBP4水平与这些临床指标的相关性。 [结果] 2型糖尿病人群与糖调节正常人群之间血清RBP4水平差异无统计学意义[(1.05±0.51)vs(0.95±0.24)μmol / l,P=0.33];而各亚组间血清RBP4水平:OW/OB-T2DM组(1.20±0.34)μmol / l OW/OB-NGR组(1.08±0.27)μmol / l NW-T2DM组(0.86±0.06)μmol / l NW-NGR组(0.81±0.07)μmol / l。其中以OW/OB-T2DM组与OW/OB-NGR组的血清RBP4水平均明显高于NW-NGR组及NW-T2DM组,差异均有统计学意义(P0.05或P0.01);但OW/OB-T2DM组与OW/OB-NGR组间,以及NW-T2DM与NW-NGR组间血清RBP4水平差异均无统计学意义。Pearson相关分析发现血清RBP4水平分别与性别(r=-0.280,P=0.009)、BMI(r=0.455,P0.001)、WHR(r=0.373,P0.001)、TG(r=0.223,P=0.038)、FINS(r=0.259,P=0.015)及HOMA-IR(r=0.415,P0.001)有显著相关性。多元逐步回归分析表明BMI(r~2=0.21,P0.001)、性别(r~2=0.17,P=0.045)是血清RBP4的独立相关因素。 [结论]肥胖人群的空腹血清RBP4水平显著升高;血清RBP4水平与性别、BMI、WHR、TG、FINS及HOMA-IR呈明显相关性,而与空腹血糖及糖化血红蛋白无显著关联,提示其可能参与了肥胖症、2型糖尿病等胰岛素抵抗相关疾病的发病机制;RBP4可能成为又一治疗2型糖尿病的作用靶点。
[Abstract]:[objective] to study the changes of serum retinol binding protein-4 (RBP4) levels in people with different glucose tolerance and body weight, and to analyze their relationship with other related metabolic and clinical biochemical indexes. The relationship between RBP4, another recently discovered fat factor, and insulin resistance, and its possible role in the development of type 2 diabetes and obesity were discussed. [methods] using enzyme-linked immunosorbent assay (Elisa) to detect OW / OB-T _ 2DM (n = 14) and OW / OB-T _ 2DM (n = 26) and OW / OB-T _ 2DM (n = 14), respectively. The level of serum retinol binding protein-4 (RBP4); Fasting plasma true insulin (FINSN), fasting blood glucose (FBGN), glycosylated hemoglobin (HbA1C), total cholesterol (TC), triglyceride (TG), high density lipoprotein cholesterol (HDL-C), low density lipoprotein cholesterol (LDL-C), systolic blood pressure (SBP), diastolic blood pressure (DBP), height, weight, The waist circumference (WH), hip circumference (H), waist to hip ratio (WHR), body mass index (BMI) were calculated, and the homeostasis model assessment method was used to calculate the insulin resistance index (HOMA-IRN): HOMA-IRG FBG 脳 fins / 22.5. SPSS11.5for windows software was used to analyze the changes of serum RBP4 level and the correlation between serum RBP4 level and these clinical indexes in different glucose regulation and body weight groups. [results] there was no significant difference in serum RBP4 level between type 2 diabetes mellitus and normal glucose regulated subjects [1.05 卤0.51)vs(0.95 卤0.24 渭 mol / L], but the serum RBP4 level among subgroups was 1.20 卤0.34) 渭 mol / l OW/OB-NGR (1.08 卤0.27) 渭 mol / l NW-T2DM (0.86 卤0.06) 渭 mol / l NW-NGR (0.81 卤0.07) 渭 mol / L. The serum RBP4 levels in OW/OB-T2DM and OW/OB-NGR groups were significantly higher than those in NW-NGR and NW-T2DM groups (P 0.05 or P 0.01), but there was no significant difference between OW/OB-T2DM group and OW/OB-NGR group. There was no significant difference in serum RBP4 levels between NW-T2DM and NW-NGR groups. Pearson correlation analysis showed that the serum RBP4 levels were significantly correlated with BMIR 0.455T 0.455N 0.455P 0.001P 0.001 (P 0.001) and HOMA-IRR 0.259P0. 0015 (P 0. 001). Multivariate stepwise regression analysis showed that BMIR2 (0.21) and sex (0.17) P0. 045) were independent correlation factors of serum RBP4. [conclusion] the fasting serum RBP4 level was significantly increased in obese people, and the serum RBP4 level was significantly correlated with BMI-WHR, TGG fins and HOMA-IR, but had no significant correlation with fasting blood glucose and glycosylated hemoglobin. These results suggest that RBP4 may be involved in the pathogenesis of insulin resistance-related diseases such as obesity, type 2 diabetes and so on. RBP4 may be another target for the treatment of type 2 diabetes.
【学位授予单位】：苏州大学
【学位级别】：硕士
【学位授予年份】：2008
【分类号】：R587.1;R589.2

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