橄榄苦苷对破骨细胞增殖影响的实验研究

发布时间：2018-05-03 11:30

本文选题：橄榄苦苷 + 破骨细胞　；参考：《中国骨质疏松杂志》2017年07期

【摘要】：目的探讨橄榄苦苷不同药物浓度及不同作用时间对破骨细胞增殖的影响。方法破骨细胞的制备,采用sRANKL与M-CSF诱导小鼠单核细胞RAW264.7细胞获得破骨细胞。设置实验组:4组分别加入400μg/mL、200μg/mL、100μg/mL、50μg/mL橄榄苦苷,另设空白对照组。运用抗酒石酸酸性磷酸酶染液试剂盒进行破骨细胞TRAP染色鉴定,并采用Cell Counting Kit法(CCK法)检测应用不同浓度橄榄苦苷及不同作用时间抑制破骨细胞增殖的情况。结果与空白对照组比较,400μg/mL、200μg/mL、100μg/mL橄榄苦苷对破骨细胞的增殖均具有明显抑制作用。当药物作用时间为24 h及72 h时,400μg/mL橄榄苦苷对破骨细胞增殖的抑制作用最强,当药物作用时间为48 h时,200μg/mL橄榄苦苷对破骨细胞增殖的抑制作用最强,差异有统计学意义(P0.05);50μg/mL橄榄苦苷对破骨细胞的增殖无明显抑制作用,差异不具有统计学意义(P0.05)。结论橄榄苦苷可能通过破坏破骨细胞细胞膜的完整性抑制破骨细胞的增殖。
[Abstract]:Objective to investigate the effects of different concentration and time of olivopicrin on the proliferation of osteoclasts. Methods Osteoclasts were prepared and osteoclasts were obtained from RAW264.7 cells induced by sRANKL and M-CSF. The experiment group (group 1: 4) was added with 400 渭 g 路mL ~ (-1) 100 渭 g 路mL ~ (-1) of 100 渭 g 路mL ~ (-1) and 50 渭 g/mL olivopicrin, respectively, and a blank control group was set up. The osteoclast TRAP staining was performed with tartrate-resistant acid phosphatase staining kit, and the inhibition of osteoclast proliferation was detected by Cell Counting Kit assay. Results compared with the control group, 400 渭 g 路mL ~ (-1) ~ 200 渭 g 路mL ~ (-1) ~ 100 渭 g/mL of olivopicrin significantly inhibited the proliferation of osteoclasts. The inhibition of osteoclast proliferation was the strongest at the time of 24 h and 72 h, and the strongest at 48 h, and the inhibitory effect on the proliferation of osteoclast was the strongest when the drug was used for 48 h, and the inhibitory effect on osteoclast proliferation was the strongest when the drug was used for 24 h and 72 h, respectively. There was no significant inhibition of osteoclast proliferation by 50 渭 g/mL olivopicrin, but there was no significant difference in the proliferation of osteoclasts. Conclusion Oligopicrin may inhibit the proliferation of osteoclasts by destroying the integrity of osteoclasts.
【作者单位】：南京军区福州总医院;厦门大学附属东方医院;
【分类号】：R580

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