高雄激素血症SD大鼠模型构建及其糖代谢表型初步探讨

发布时间：2018-05-04 18:45

  本文选题：雄激素 + 糖耐量受损　； 参考：《南京医科大学》2015年硕士论文

【摘要】：糖尿病是一种由于胰岛素绝对或相对不足引起的以高血糖为主要特征的代谢性疾病。目前,糖尿病已成为严重威胁人类健康的全球性公共卫生问题。糖耐量受损被认为是糖尿病的早期表现,可定义为前期糖尿病。胰岛素抵抗是2型糖尿病的重要特征,也是2型糖尿病发生发展的关键因素。过量雄激素导致女性肥胖、胰岛素抵抗的现象受到人们的广泛关注,而人们对高雄激素血症对于男性糖代谢的影响却缺乏足够的重视。同时现今社会雄激素滥用的现象日益严重,外源性过量摄入雄激素对男性机体糖代谢产生的影响亟需进一步探索。我们以SD大鼠为动物模型,探讨了长期超生理水平的雄激素对糖耐量及胰岛素敏感性的影响。我们首先借鉴以往研究,以雌性SD大鼠为模型,筛选出合适的激素缓释管并验证了其体内埋植效果,从而确立了合适的造模方法。实验过程中,我们发现用医用粘合剂制作的激素缓释管(缓释管Ⅱ)明显优于木质材料封闭两端的缓释管(缓释管Ⅰ),并且证明了缓释管Ⅱ可在SD大鼠体内正常工作。基于以上几点,我们用缓释管Ⅱ构建了高DHT血症雄性大鼠模型,并且首次发现长期(3个月,3 m)超生理浓度DHT可导致雄性SD大鼠糖耐量受损。结合胰岛素耐量实验及原代胰岛GSIS(Glucose-stimulated insulin secretion,葡萄糖刺激胰岛素分泌)功能检测等实验,我们发现其糖耐量受损可能是DHT以间接方式损伤胰岛β细胞功能造成的。综上所述,长期(3 m)超生理浓度DHT可引起雄性SD大鼠糖耐量受损及胰岛β细胞功能障碍。本研究将有助于加深人们对雄激素副作用的认识,并对雄激素滥用者起到一定警醒作用。
[Abstract]:Diabetes is a metabolic disease characterized by hyperglycemia due to absolute or relative deficiency of insulin. At present, diabetes has become a serious threat to human health global public health problems. Impaired glucose tolerance is considered an early manifestation of diabetes and can be defined as prediabetes. Insulin resistance is an important feature of type 2 diabetes and a key factor in the development of type 2 diabetes. Excessive androgen leads to obesity and insulin resistance in women. However, the effect of hyperandrogenemia on glucose metabolism in men is not paid enough attention to. At the same time, the phenomenon of androgen abuse is becoming more and more serious, and the effect of exogenous excessive intake of androgen on glucose metabolism of male body needs to be further explored. We used SD rats as an animal model to study the effects of long-term hyperphysiological androgen on glucose tolerance and insulin sensitivity. Based on the previous studies, we selected suitable steroid sustained-release tubes for female SD rats, and verified the implantation effect in vivo, thus establishing a suitable modeling method. In the course of the experiment, we found that the hormone sustained-release tube (鈪，

本文编号：1844190