多药耐药蛋白1和多药耐药相关蛋白5、7在糖尿病小鼠视网膜中表达的变化

发布时间：2018-05-07 15:10

本文选题：糖尿病视网膜病变 + 血-视网膜内屏障　；参考：《眼科新进展》2017年01期

【摘要】：目的观察多药耐药蛋白1(multidrug resistance 1,MDR1)、多药耐药相关蛋白(multidrug resistance-associated protein,MRP)5和MRP7在不同时期糖尿病小鼠视网膜中的表达变化。方法通过腹腔注射链脲佐菌素诱导C57BL/6小鼠建立糖尿病模型,伊文思蓝灌注检测血-视网膜内屏障变化。分别于糖尿病造模成功后的4周、12周和24周取小鼠视网膜,进行实时定量-PCR、免疫荧光、Western blotting等实验,检测各组小鼠视网膜中MDR1、MRP5和MRP7的表达变化。结果伊文思蓝灌注结果显示在糖尿病12周就出现视网膜渗漏;实时定量-PCR结果发现与对照组相比,随糖尿病进展,MDR1、MRP5和MRP7在4周、12周和24周的表达均呈下降趋势(MDR1:P_(4周)=0.028,P_(12周)=0.003,P_(24周)0.001;MRP5:P_(4周)=0.045,P_(12周)=0.009,P_(24周)0.001;MRP7:P_(4周)=0.019,P12周0.001,P24周=0.001),在糖尿病不同时期其变化也呈下降趋势(均为P0.05);免疫荧光观察到MDR1、MRP5和MRP7的荧光强度随糖尿病发展逐渐减弱;Western blotting检测可见在4周、12周、24周糖尿病小鼠视网膜中MDR1、MRP5和MRP7的表达均呈下降趋势。结论在DR早期血-视网膜内屏障会受到破坏,视网膜中外排转运蛋白的表达也会受糖尿病的影响导致不同程度的下降。
[Abstract]:Objective to observe the expression of multidrug resistance protein (1(multidrug resistance 1), multidrug associated protein (resistance-associated), MRP5 and MRP7 in the retina of diabetic mice at different stages. Methods Diabetic model was established in C57BL/6 mice by intraperitoneal injection of streptozotocin. Evans blue perfusion was used to detect the changes of blood-retinal barrier. The retina of mice was harvested at 4 weeks, 12 weeks and 24 weeks after the successful establishment of diabetes mellitus. The expression of MDR1 MRP5 and MRP7 in the retina of each group was detected by real-time quantitative -PCR and immunofluorescence Western blotting. Results the results of Evans blue perfusion showed that retinal leakage occurred at 12 weeks after diabetes, and real-time quantification-PCR results showed that compared with the control group, The expression of MDR1 / MRP5 and MRP7 decreased in 4 weeks, 12 weeks and 24 weeks, respectively. The expression of MDR1P5 and MRP7 showed a downward trend in 4 weeks, 0.028 P0. 028 P0. 12 weeks, 0. 003P0. 24 weeks, 0. 001: MRP5: P0. 044, 0. 009P5, 0. 001, 0. 001, 0. 019P12, 0. 001P24, 0. 0011, and 0. 001P: 0. 001. the changes showed a downward trend (P 0. 05; P 0. 05; P 0. 05; P 0. 05; P 0. 05, P 0. 05). It was observed that the fluorescence intensity of MDR1-MRP5 and MRP7 decreased with the development of diabetes mellitus. The expression of MDR1-MRP5 and MRP7 in the retina of diabetic mice decreased in 4 weeks, 12 weeks and 24 weeks. Conclusion in the early stage of Dr, the blood-retinal barrier will be destroyed, and the expression of retinal efflux transporter will be affected by diabetes mellitus.
【作者单位】：济南大学山东省医学科学院医学与生命科学学院;山东省眼科研究所;
【基金】：国家自然科学基金资助(编号:81271036,81500751) 2015年度山东省重点研发计划资助(编号:2015GSF11-8121) 山东省医学科学院医药卫生科技创新工程~~
【分类号】：R587.2;R774.1

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