成纤维细胞生长因子21对胰岛素抵抗肝细胞葡萄糖摄取的影响及机制

发布时间：2018-05-10 18:05

本文选题：成纤维细胞生长因子 + 葡萄糖转运蛋白　；参考：《山东医药》2017年26期

【摘要】：目的观察体外合成成纤维细胞生长因子21(FGF21)对胰岛素抵抗(IR)肝细胞葡萄糖摄取的影响,并探讨其机制。方法体外合成具有一定稳定性的荧光标记的FGF21 mRNA。将肝细胞置于含34.4μg/mL重组胰岛素和1μg/mL地塞米松及10%FBS的RPMI-1640培养基中培养72 h,诱导建立IR肝细胞模型,将其分为模型对照组,胰岛素组和FGF21组。模型对照组不添加任何药物,胰岛素组加入1μg/mL重组胰岛素,FGF21组电转入FGF21 mRNA。采用葡萄糖氧化酶法测定3组细胞培养液中的葡萄糖含量,计算葡萄糖吸收量;实时荧光定量PCR方法检测3组葡萄糖转运蛋白1(GLUT1)mRNA表达,Western blotting法检测3组GLUT1蛋白表达。结果与模型对照组相比,FGF21组葡萄糖吸收量升高,细胞GLUT1 mRNA及蛋白表达升高(P均0.05)。与模型对照组相比,胰岛素组葡萄糖吸收量不明显,细胞GLUT1 mRNA及蛋白表达无明显差异(P均0.05)。结论体外合成的FGF21 mRNA可以改善IR肝细胞对葡萄糖的摄取,其机制与增加GLUT1表达有关。
[Abstract]:Objective to investigate the effect of fibroblast growth factor 21 FGF21 on glucose uptake in insulin-resistant (IRN) hepatocytes and its mechanism. Methods FGF21 mRNA with certain stability was synthesized in vitro. Hepatocytes were cultured in RPMI-1640 medium containing 34.4 渭 g/mL recombinant insulin and 1 渭 g/mL dexamethasone and 10s for 72 hours. The model of IR hepatocytes was induced and divided into three groups: model control group, insulin group and FGF21 group. Model control group did not add any drugs, insulin group added 1 渭 g/mL recombinant insulin FGF21 group into FGF21 mRNA. Glucose content in culture medium was measured by glucose oxidase method and glucose absorption was calculated. 1(GLUT1)mRNA expression of glucose transporter protein in three groups was detected by real-time fluorescence quantitative PCR and GLUT1 protein expression in three groups was detected by Western blotting method. Results compared with the model control group, the glucose absorption and the expression of GLUT1 mRNA and protein in the FGF21 group were increased (P < 0.05). Compared with the model control group, the glucose absorption of insulin group was not obvious, and the expression of GLUT1 mRNA and protein were not significantly different between insulin group and model control group (P < 0.05). Conclusion the synthesis of FGF21 mRNA in vitro can improve the glucose uptake of IR hepatocytes, and its mechanism is related to the increase of GLUT1 expression.
【作者单位】：天津医科大学;武警广西总队医院;天津市美联博科技有限公司;
【基金】：天津市科技计划项目(14ZCZDCX00054)
【分类号】：R587.1

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