脂代谢相关基因多态性与长期有氧运动老年人高脂血症的相关性研究

发布时间：2018-05-11 00:35

  本文选题：基因多态性 + 高血脂　； 参考：《上海体育学院》2016年硕士论文

【摘要】：研究目的通过查阅文献,选取了7个与血脂及运动相关的SNP位点(APOA5基因rs662799、AVPV1α基因rs1042615、GHRL基因rs26802、NOS3基因rs2070744以及IL基因上的rs2228059、rs1589241和rs1800795),欲在长期进行有氧运动的老年人群中,探讨这些位点与高脂血症的关联性,并针对有关联的位点,分层研究不同基因型与体力活动对血脂水平的影响作用,从而可以为高血脂患者制定个性化的运动处方提供科学的理论依据。研究方法从上海市杨浦区、虹口区、徐汇区、浦东新区四个辖区的部分社区招募有长期规律运动史的老年人。纳入标准:(1)年龄介于60~69岁;(2)身体健康,无严重心脑血管疾病且近期未接受调脂类药物治疗;(3)规律运动史:从事规律运动锻炼3年以上,且每次锻炼时间不低于30min,每周运动次数不少于3次。通过初筛问卷和进一步体格检查,最终纳入符合条件的受试者共计432例,剔除部分信息缺失者9例后,实际有效受试者共计423例。其中男性182例,女性241例,最小年龄60岁,最大年龄69岁,平均年龄63.5±3.1岁。采集受试者空腹静脉血,分别采用胆固醇氧化酶法、GPO-POD酶法及酶促显色法测定胆固醇、甘油三酯和低高密度脂蛋白胆固醇的水平,并对受试者进行基本的体格测试(身高、体重、BMI等)。根据2007年制定的《中国成人血脂异常防治指南》血脂异常的界定标准,结合问卷和血脂测定结果,把受试者分为高血脂组和血脂正常组(对照组)。应用基质辅助激光解吸附电离飞行时间质谱检测技术(MALDI-TOF)对预先挑选的影响脂代谢的多态位点进行基因分型。体力活动水平通过《国际体力活动量表(International Physical Activity Questionnaire,IPAQ)中文版长卷》进行测量,并按照IPAQ制定的标准,计算每周体力活动能量消耗的MET值。研究结果1.高血脂组的总胆固醇、甘油三酯、低密度脂蛋白水平均显著高于对照组(P0.001),而高密度脂蛋白水平则显著低于对照组(P0.001),两组间的体重指数、血压、血糖及各体力活动水平的人群分布均没有统计学差异(P0.05)。2.除了rs1800795位点未发现多态性外,其他6个位点均符合Hardy-Weinberg平衡定律(P0.05),仅APOA5基因rs662799位点的基因型及等位基因频率在高血脂组和对照组中的分布差异有统计学意义(χ2=11.233,P=0.004,χ2=11.698,P=0.001),其他5个多态位点在两组间的分布情况均未表现出统计学差异(P0.05)。3.利用多元logistic回归分析,校正了性别、年龄、BMI及体力活动水平后发现,rs662799位点的三种基因型中,AG和GG基因型携带者患高血脂的风险分别是AA基因型的1.676倍(P=0.018,95%CI:1.092-2.571)和1.812倍(P=0.002,95%CI:1.247-2.632),进一步利用线性回归分析rs662799与血脂水平的关联性发现,G等位基因的存在与TG水平呈显著正相关(P0.001)、与HDL水平显著负相关(P=0.001)。单因素方差分析结果显示,AA、AG和GG基因型个体的TG水平依次升高,分别为1.30±0.56mmol/L、1.68±0.82mmol/L和1.99±0.94 mmol/L,且AG和GG基因型个体的水平均显著高于AA基因型个体(P0.01);而AA、AG和GG基因型个体的HDL水平依次降低,分别为1.43±0.37 mmol/L、1.36±0.31 mmol/L和1.28±0.28 mmol/L,且GG基因型个体的水平显著高于AA基因型个体(P0.05)。4.利用四分位法将体力活动水平进行分级后发现,受rs662799基因多态性的影响,体力活动与TG水平的关系不同:AA基因型携带人群,在不同体力活动水平下,TG水平均未有统计学差异(P0.05),而AG+GG基因型携带人群在不同体力活动水平下,TG水平具有显著性差异(P0.05),当体力活动水平高于8134.5 MET-min/w时,TG出现了最低水平(1.45±0.74)mmol/L,较体力活动水平低于5460 MET-min/w时显著降低(P0.05)。研究结论1.APOA5基因rs662799单核苷酸多态性与长期有氧锻炼老年人的高血脂存在密切关联,G等位基因可能是高血脂发生的易感基因。2.体力活动与血脂水平的关系可能受到rs662799基因多态性的影响,AA基因型携带人群的TG水平受体力活动的影响不大,而AG和GG基因型人群可能在较高水平(PA8134.5 MET-min/W)的体力活动下,可以有效改善TG水平。
[Abstract]:The purpose of this study was to select 7 SNP loci related to blood lipid and exercise (APOA5 gene rs662799, AVPV1 a gene rs1042615, GHRL gene rs26802, NOS3 gene rs2070744 and IL gene rs2228059, rs1589241, and IL). The effects of different genotypes and physical activity on the level of blood lipids were studied in stratification, which could provide scientific theoretical basis for the formulation of personalized exercise prescriptions for hyperlipidemia patients. The research methods were recruited from four communities in Yangpu District, Hongkou District, Xuhui District and Pudong New Area, Shanghai. The elderly with a history of regular exercise history included standard: (1) age between 60~69 years; (2) healthy, no serious cardio cerebral vascular disease and no lipo therapy in the near future; (3) regular exercise history: regular exercise for more than 3 years, and each exercise is not less than 30min, no less than 3 times a week. Through the initial screening questionnaire And further physical examination, a total of 432 eligible subjects were included. After 9 cases of missing information, the total number of effective subjects was 423 cases, including 182 males and 241 females, the minimum age of 60 years, the maximum age of 69, and the average age of 63.5 + 3.1 years. The levels of cholesterol, triglycerides, and low density lipoprotein cholesterol were measured by GPO-POD enzyme method and enzymatic colorimetric method, and the subjects were tested with basic physical tests (height, weight, BMI, etc.). According to the guidelines for the prevention and control of dyslipidemia in Chinese adults and the criteria for the determination of blood lipids in 2007, the results of the questionnaire and blood lipid determination were combined. The subjects were divided into hyperlipidemia group and normal blood lipid group (control group). Using matrix assisted laser desorption ionization time of flight mass spectrometry (MALDI-TOF), the pre selected polymorphic loci affecting lipid metabolism were genotyping. The level of physical activity was measured by the international physical activity scale (International Physical Activity Questionnaire, IP). AQ) the Chinese version of long scroll > measured and calculated the MET value of energy consumption of physical activity per week according to the standard set by IPAQ. Results the total cholesterol, triglyceride, low density lipoprotein level of the 1. hyperlipidemia group were significantly higher than that of the control group (P0.001), while the high density lipoprotein level was significantly lower than that of the control group (P0.001), and the weight of the two groups between the high blood fat groups was significantly lower than that of the control group (P0.001). The distribution of index, blood pressure, blood glucose and the level of physical activity were not statistically different (P0.05).2., except for the polymorphism of rs1800795 loci, the other 6 loci were consistent with the Hardy-Weinberg equilibrium law (P0.05), and the distribution of genotype and allele frequency in the rs662799 locus of APOA5 gene in the hyperlipidemia group and the control group was poor. The difference was statistically significant (x 2=11.233, P=0.004, X 2=11.698, P=0.001), and the distribution of the other 5 polymorphic loci in the two groups was not statistically significant (P0.05).3. using multiple logistic regression analysis to correct the sex, age, BMI and physical activity levels, and the three genotypes of the rs662799 loci, AG and GG, were carried. The risk of hyperlipidemia was 1.676 times (P=0.018,95%CI:1.092-2.571) and 1.812 times (P=0.002,95%CI:1.247-2.632) of the AA genotype, respectively. Further using linear regression analysis the correlation between rs662799 and blood lipid levels, the existence of G allele was significantly positively correlated with the level of TG (P0.001), and was negatively correlated with the level of HDL (P=0.001). The results of the prime variance analysis showed that the levels of TG in AA, AG and GG genotype individuals were increased in turn, 1.30 + 0.56mmol/L, 1.68 + 0.82mmol/L and 1.99 + 0.94 mmol/L respectively, and the levels of AG and GG genotype individuals were significantly higher than those of AA genotype individuals (P0.01), while AA, 1.43 + 0.37, 1.36, 1.36, respectively, 1.36, 1.36, respectively. 1.36 0.31 mmol/L and 1.28 + 0.28 mmol/L, and the level of GG genotype individuals was significantly higher than that of AA genotype individuals (P0.05).4. using four division method to classify the level of physical activity. The relationship between physical activity and TG level was different from that of rs662799 gene polymorphism: AA based type carrying population, TG water at different physical activity levels. There was no statistical difference (P0.05) on average (P0.05), while AG+GG genotype had significant difference (P0.05) at different levels of physical activity (P0.05). When the level of physical activity was higher than 8134.5 MET-min/w, TG had the lowest level (1.45 + 0.74) mmol/L, which was significantly lower than that of physical activity below 5460 MET-min/w (P0.05). Conclusions 1. The single nucleotide polymorphism of the APOA5 gene rs662799 is closely related to the high blood lipid in the elderly with long-term aerobic exercise. The G allele may be a susceptible gene of hyperlipidemia, the relationship between the physical activity of.2. and the blood lipid level may be affected by the polymorphism of the rs662799 gene. The TG level of the AA genotype group is not affected by physical activity. Large and AG and GG genotypes may be effective at improving TG levels at higher levels (PA8134.5 MET-min/W).

【学位授予单位】：上海体育学院
【学位级别】：硕士
【学位授予年份】：2016
【分类号】：R589.2
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本文编号：1871648