L型钙离子通道阻滞剂贝尼地平对去卵巢小鼠骨代谢的研究

发布时间：2018-05-17 14:17

本文选题：骨代谢 + 骨质疏松　；参考：《中国骨质疏松杂志》2017年08期

【摘要】：目的探讨贝尼地平对去卵巢骨质疏松模型小鼠的骨代谢影响。方法随机选取鼠龄10周雌性小鼠30只,均分为假手术组,单纯去卵巢组,去卵巢加药组。假手术组只去除卵巢周围少量脂肪,单纯去卵巢组去除卵巢后灌胃等量生理盐水,去卵巢加药组去除卵巢后灌胃贝尼地平[20 mg/(kg·d),benidipine,BD]3个月。取小鼠股骨,经4%多聚甲醛固定,脱钙后分别进行股骨下段石蜡切片伊红苏木素(HE)染色、转录因子RUNX2(runt-related transcription factor 2,Runx2)免疫组织化学染色及骨钙素(osteocalcin,OCN)免疫荧光(immunofluorescence,IF)染色,并进行显微CT(μCT)扫描。结果股骨下段HE染色发现灌胃贝尼地平的去卵巢小鼠股骨下段骨小梁面积明显高于单纯去卵巢小鼠组(OVX组)(P0.05),差异具有统计学意义,和假手术组相比,去卵巢加药组股骨下段骨小梁明显降低,差异具有统计学意义(P0.05);股骨下段RUNX2免疫组化染色发现,和单纯切卵巢组小鼠相比,贝尼地平治疗组的蛋白表达量明显上调,差异具有统计学意义(P0.05),而各组中,假手术组RUNX2表达量仍然是最高的,差异具有统计学意义(P0.05);通过骨钙素免疫荧光染色发现,贝尼地平治疗组股骨下段骨钙素表达量明显高于去卵巢组,差异具有统计学意义(P0.05),但是仍低于假手术组,差异具有统计学意义(P0.05);此外,通过股骨下段显微CT扫描发现,去卵巢小鼠灌胃贝尼地平组股骨下段骨密度(bone mineral density,BMD),骨小梁厚度(trabecular thickness,Tb.Th),骨小梁数量(trabecular number,Tb.N)均明显高于去卵巢组,差异均具有统计学意义(P0.05),但骨代谢指标仍低于假手术组,差异均具有统计学意义(P0.05)。结论贝尼地平能明显减缓由于去卵巢引起的C57/BL6雌鼠骨量丢失,具有比较明显的抗骨质疏松作用。
[Abstract]:Objective to investigate the effect of benidipine on bone metabolism in ovariectomized osteoporosis mice. Methods Thirty female mice aged 10 weeks were randomly divided into sham-operation group, ovariectomized group and ovariectomized group. The sham operation group only removed a small amount of fat around the ovary, the simple ovariectomized group removed the ovary, and the ovariectomized group was given the same amount of normal saline after removing the ovary, and the ovariectomized plus drug group was given benidipine [20 mg/(kg] for 3 months. The femur of the mice was fixed with 4% paraformaldehyde, and then decalcified with eosylcyrrhizin (HE) staining, RUNX2(runt-related transcription factor _ 2 Runx _ 2) immunohistochemical staining and osteocalcin OCN-immunofluorescence (IFIFF) staining, and the microCTs were scanned by microCTs (渭 CTs). Results the area of trabecular bone in ovariectomized mice fed with benidipine was significantly higher than that in OVX group (P 0.05), and the difference was statistically significant compared with sham operation group. In the ovariectomized group, the trabecular bone of the lower femur was significantly decreased, the difference was statistically significant (P 0.05), and the expression of protein in benidipine treatment group was significantly higher than that in the simple ovariectomized group, and the RUNX2 immunohistochemical staining in the lower femur segment of the femur was significantly higher than that in the ovariectomized group. The difference was statistically significant (P 0.05), but the expression of RUNX2 in sham operation group was still the highest (P 0.05), which was found by osteocalcin immunofluorescence staining. The expression of osteocalcin in the lower femur of the benidipine group was significantly higher than that in the ovariectomized group, the difference was statistically significant (P 0.05), but still lower than that in the sham operation group (P 0.05). In ovariectomized mice, the bone mineral density, trabecular thickness, trabecular thickness and trabecular number Tb. N were significantly higher in the ovariectomized group than in the ovariectomized group. The difference was statistically significant (P 0.05), but the bone metabolism index was still lower than that in the sham operation group. The difference was statistically significant (P 0.05). Conclusion Benidipine can significantly reduce bone loss in ovariectomized C57/BL6 female rats and has obvious anti-osteoporosis effect.
【作者单位】：内蒙古医科大学第二附属医院关节外科;
【分类号】：R580

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