去卵巢骨质疏松大鼠骨组织肥大细胞增生及其病理学意义

发布时间：2018-05-17 21:49

本文选题：大鼠 + 去卵巢　；参考：《中国骨质疏松杂志》2017年07期

【摘要】：目的观察肥大细胞在去卵巢骨质疏松大鼠骨组织中的分布,探讨肥大细胞增生与绝经后骨质疏松症之间的关系。方法 3月龄健康雌性SD大鼠20只,随机分为假手术组(Sham组)和去卵巢组(OVX组),卵巢去除后3个月,采集和分离血清样品测定血清雌二醇、碱性磷酸酶(alkaline phosphatase,ALP)、骨保护素(osteoprotegerin,OPG)和核因子κB受体活化因子配体(receptor activator of NF-κB ligand,RANKL),收集股骨近端干骺端、胫骨近端干骺端组织样品进行常规组织切片制作和肥大细胞的甲苯胺蓝染色,并计数单位面积肥大细胞的数量。结果 OVX组大鼠血清雌二醇、OPG含量较Sham组显著降低(P0.01),血清ALP、RANKL含量较Sham组显著增高(P0.01);组织化学染色结果显示肥大细胞主要分布于大鼠胫骨、股骨骨髓腔中,OVX大鼠胫骨、股骨骨髓腔肥大细胞数量较Sham组显著增多(P0.01)。结论 3月龄大鼠卵巢去除后3个月呈现骨质疏松症状,骨髓腔肥大细胞数量与骨质疏松呈正相关,可作为绝经后骨质疏松症诊断和治疗的依据。
[Abstract]:Objective to observe the distribution of mast cells in bone tissue of ovariectomized osteoporosis rats and to explore the relationship between mast cell proliferation and postmenopausal osteoporosis. Methods Twenty healthy female Sprague-Dawley rats aged 3 months were randomly divided into sham-operated group (Sham group) and ovariectomized group (OVX group). Serum estradiol was collected and separated 3 months after ovariectomy. Alkaline phosphatase (ALP), osteoprotegerin (OPG) and nuclear factor- 魏 B receptor activating factor ligand (activator of NF- 魏 B ligand RANKL) were collected from proximal femoral metaphysis and proximal tibial metaphysis for routine tissue sections and toluidine blue staining of mast cells. The number of mast cells per unit area was counted. Results compared with Sham group, the content of estradiol in serum of OVX group was significantly lower than that of Sham group, and the content of serum ALPRANKL was significantly higher than that of Sham group, the histochemical staining showed that mast cells were mainly distributed in the tibia of rats and the tibia of OVX rats in femoral medullary cavity. The number of mast cells in the medullary cavity of the femur was significantly higher than that in the Sham group (P 0.01). Conclusion Osteoporosis was found in 3 months old rats after ovarian removal, and the number of mast cells in medullary cavity was positively correlated with osteoporosis, which could be used as a basis for diagnosis and treatment of postmenopausal osteoporosis.
【作者单位】：陕西理工大学维生素D生理与应用研究所;
【基金】：陕西省科技创新团队项目(2012KCT-29) 陕西省科协青年人才托举项目(20160128) 陕西理工大学大学生创新创业项目(2017037);陕西理工大学博士后项目(SLGBH16-03)
【分类号】：R580

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