调节性B细胞在HIV-1感染中的作用研究

发布时间：2018-05-17 20:52

本文选题：艾滋病病毒I型感染者/艾滋病患者 + 调节性B细胞　；参考：《大理大学》2017年硕士论文

【摘要】：目的:本研究旨在观察调节性B细胞在HIV-1/AIDS患者外周血中的变化,探讨其在HIV-1/AIDS疾病中的发病机制和疾病发展进程中的作用。研究方法:本项研究以云南省传染病专科医院住院部和门诊部的HIV-1/AIDS患者为实验组,以医疗卫生工作者和健康体检人群为对照组。选择121例未经过抗病毒治疗的HIV-1/AIDS患者,分成两组,其中无症状感染者82人、艾滋病患者39例,接受HAART治疗并长期随访者30例(在121例HIV-1/AIDS患者中选择)。用流式细胞术(FCM)检测其外周血中CD1d hiCD5+CD19+B细胞的比例、CD4+T淋巴细胞数量、CD8+T淋巴细胞数量、CD4细胞/CD8细胞的比值,并与同期选择的36例健康对照者作比较。通过研究HIV-1/AIDS患者和健康对照者横断面队列外周血中T淋巴细胞数量和调节性B细胞比例变化,以及其中的30例HIV-1/AIDS患者在接受HAART前、后不同时期T淋巴细胞数量和Bergs比例的表达水平变化,并且观察30例HIV-1/AIDS患者接受HAART长期随访过程中艾滋病的纵向进展和抗病毒治疗疗效的关系,统计分析组内各变量间的相关性。结果:HIV-1/AIDS患者外周血中CD4细胞数量显著低于健康对照组(P0.001),AIDS病人的CD4细胞数量显著低于HIV-1感染组(P0.001)。HIV-1/AIDS患者外周血中CD8细胞数量显著低于健康对照组(P0.001),而AIDS病人的CD8细胞数量与HIV-1感染者相比无显著差异(P=0.709)。HIV-1/AIDS患者外周血中CD4细胞/CD8细胞比值显著低于健康对照组(P0.001),AIDS病人的CD4细胞/CD8细胞比值显著低于HIV-1感染者(P0.001)。HIV-1/AIDS患者外周血中CD1d hiCD5+CD19+B细胞的比例显著高于健康对照组(P0.01),AIDS患者的外周血中的CD1d hiCD5+CD19+B细胞的比例显著高于HIV-1感染者(P0.001)。并且HIV-1/AIDS患者外周血中CD1d hiCD5+CD19+B细胞的比例与CD4细胞数量(r=-0.732,r2=0.536,P0.001)和CD8细胞数量(r=-0.253,r2=0.064,P=0.011)均呈负相关关系,或者与CD4细胞/CD8细胞的比值(r=-0.643,r2=0.414,P0.001)呈负相关关系。30例接受HAART患者在治疗前、治疗后的2周、1个月、2个月、3个月、6个月CD1d hiCD5+CD19+B细胞的比例均与同时期的CD4细胞数量、CD8细胞数量呈负相关关系,并且在HAART治疗后CD4细胞均值有上升趋势,CD1d hiCD5+CD19+B细胞比例均值有下降趋势。结论:HIV-1/AIDS患者外周血中CD1d hiCD5+CD19+B细胞显著升高,并随着艾滋病的进展而明显增加,经过抗病毒治疗后下降。CD1d hiCD5+CD19+B细胞可能在艾滋病的免疫衰竭中发挥作用,促进了艾滋病的发展进程;针对性降低HIV-1/AIDS患者的CD1d hiCD5+CD19+B细胞比例可能有利于抑制艾滋病的发展进程。
[Abstract]:Aim: to observe the changes of regulatory B cells in peripheral blood of patients with HIV-1/AIDS, and to explore the role of B cells in the pathogenesis and progression of HIV-1/AIDS disease. Methods: in this study, the HIV-1/AIDS patients in the inpatient and outpatient departments of Yunnan Provincial Infectious Diseases Hospital were taken as the experimental group, and the medical and health workers and the healthy people as the control group. One hundred and twenty-one patients with HIV-1/AIDS without antiviral therapy were divided into two groups: 82 asymptomatic infection patients, 39 AIDS patients, and 30 patients who received HAART treatment and long-term follow-up (selected from 121 HIV-1/AIDS patients). The ratio of CD1d hiCD5 CD19 B cells in peripheral blood and the ratio of CD4 T lymphocytes to CD8 T lymphocytes and the ratio of CD4 / CD8 cells to CD4 / CD8 cells in peripheral blood were determined by flow cytometry (FCM) and compared with 36 healthy controls selected at the same time. The changes of T lymphocyte count and regulatory B cell ratio in peripheral blood of patients with HIV-1/AIDS and healthy controls were studied, and 30 patients with HIV-1/AIDS were treated with HAART. The expression level of T lymphocytes and Bergs ratio in 30 patients with HIV-1/AIDS were observed during the long term follow-up with HAART. The relationship between the longitudinal progression of AIDS and the efficacy of antiviral therapy was observed. The correlation between the variables in the statistical analysis group. Results the number of CD4 cells in the peripheral blood of patients with HIV / AIDS was significantly lower than that of the healthy control group (P 0.001). The number of CD8 cells in the peripheral blood of patients with HIV 1 / AIDS was significantly lower than that of the patients with HIV-1 infection. The number of CD8 cells in the peripheral blood of patients with HIV / AIDS was significantly lower than that of the healthy control group (P 0.001), while the number of CD8 cells in AIDS patients was significantly lower than that in the patients with HIV-1 infection. The ratio of CD4 cell / CD8 cell in peripheral blood of HIV / AIDS patients was significantly lower than that of the healthy control group (P 0.001). The ratio of CD4 cell / CD8 cell was significantly lower than that of HIV-1 infected patients (P 0.001n. HIV-1 / AIDS). The ratio of CD1d hiCD5 CD19 B in peripheral blood of HIV / AIDS patients with HIV / AIDS was significantly lower than that of patients with HIV / AIDS. The percentage of CD1d hiCD5 CD19 B cells in peripheral blood of P0.01AIDS-infected patients was significantly higher than that of P0.01AIDS-infected patients with HIV-1. Moreover, the ratio of CD1d hiCD5 CD19 B cells in peripheral blood of HIV-1/AIDS patients was negatively correlated with the number of CD4 cells and the number of CD8 cells, respectively. There was a negative correlation between the percentage of CD1d hiCD5 CD19 B cells in peripheral blood and the number of CD4 cells in patients with HIV-1/AIDS, or with the ratio of CD4 cells to CD8 / T cells, r-0.643r2r20.4414P0.001). 30 patients received HAART before the treatment, and 30 patients received HAART before the treatment, and the ratio of CD4 cells to CD8 / T cells was negatively correlated with the ratio of CD4 cells to CD8 / T cells (r -0.643r2tir _ (0.414) P _ (0.001). At 2 weeks, 1 month, 2 months, 3 months, 6 months after treatment, the proportion of CD1d hiCD5 CD19 B cells was negatively correlated with the number of CD4 cells and CD8 cells in the same period. After HAART treatment, the mean value of CD4 cells increased and the ratio of CD1d hiCD5 CD19 B cells decreased. Conclusion CD1d hiCD5 CD19 B cells in the peripheral blood of HIV / AIDS patients were significantly increased and increased with the progression of HIV / AIDS. After antiviral therapy, CD1d hiCD5 CD19 B cells may play a role in the immune failure of AIDS. It can promote the development of AIDS and reduce the proportion of CD1d hiCD5 CD19 B cells in HIV-1/AIDS patients.
【学位授予单位】：大理大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R512.91

【相似文献】