高活性小牛血去蛋白提取物对糖尿病大鼠肾损伤的保护作用

发布时间：2018-05-19 00:33

本文选题：小牛血去蛋白提取物 + 糖尿病肾病　；参考：《吉林大学学报(医学版)》2017年02期

【摘要】：目的:观察小牛血去蛋白提取物(DECB)对糖尿病大鼠肾损伤的保护作用,并初步探讨其作用机制。方法:Wistar雄性大鼠腹腔注射STZ(65mg·kg~(-1))建立糖尿病模型,将成模大鼠随机分为模型(M)组、二甲双胍(MMet,105 mg·kg~(-1))组、低剂量DECB联合给药(ML,105 mg·kg~(-1)二甲双胍+94.5mg·kg~(-1) DECB)组、中剂量DECB联合给药(MM,105mg·kg~(-1)二甲双胍+189mg·kg~(-1) DECB)组和高剂量联合给药(MH,105mg·kg~(-1)二甲双胍+378mg·kg~(-1) DECB)组(n=10),另取10只Wistar大鼠作为正常对照(NC)组(n=10)。二甲双胍为经口灌胃给药,DECB为腹腔注射给药,每天1次,共给药8周,NC组和M组大鼠给予等体积的生理盐水。测定各组大鼠体质量、血糖水平,检测各组大鼠血清中高密度脂蛋白胆固醇(HDL-C)、低密度脂蛋白胆固醇(LDL-C)、尿素氮(BUN)、尿微量白蛋白(UAlb)、尿肌酐(UCr)、血肌酐(SCr)、总胆固醇(TC)、甘油三酯(TG)、尿酸(UA)、谷胱甘肽(GSH)、丙二醛(MDA)水平和超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)活性;HE染色观察各组大鼠肾脏组织病理形态学表现。结果:与NC组比较,M组大鼠体质量降低,空腹血糖、UAlb、UCr、SCr、UA、BUN、LDL-C、TC、TG和MDA水平升高(P0.05),HDL-C和GSH水平降低(P0.05),SOD和GSH-Px活性降低(P0.05)。与M组比较,MM和MH组大鼠体质量升高(P0.05),血糖、UAlb、UCr、SCr、UA、BUN、LDL-C、TC、TG和MDA水平降低(P0.05),HDL-C和GSH水平升高(P0.05),SOD和GSH-Px活性升高(P0.05)。肾脏组织病理观察,与NC组比较,M组大鼠肾脏组织病变明显,肾小球充血,肾小管水肿;与M组比较,各给药组大鼠肾脏组织病变明显好转,肾小管空泡变性减轻,间质增生不明显。结论:DECB联合二甲双胍给药可降低糖尿病大鼠血糖,调节血脂,改善糖尿病大鼠肾组织病理学变化,减轻肾脏损伤,提高机体的抗氧化能力。
[Abstract]:Aim: to observe the protective effect of calf blood deproteinization extract (DECB) on renal injury in diabetic rats and to explore its mechanism. Methods Diabetic model was established by intraperitoneal injection of STZ(65mg kg-1 in male Wistar rats. The model rats were randomly divided into two groups: model group, metformin group (105mg / kg) and low dose DECB combined with MLL 105mg / kg ~ (-1) 94.5mg / kg ~ (-1) DECB) group, and then the rats were randomly divided into two groups: the model group (n = 10), the control group (n = 10), and the control group (n = 10). Middle dose DECB combined with MMMA 105mg / kg (1) metformin 189mg (KG) DECB) and high dose DECB (105mg / kg) DEC-1) metformin (378mg / kg ~ (-1) DECB) group (n = 10), 10 Wistar rats were used as normal control group (n = 10). Metformin was given orally and DECB was intraperitoneally injected once a day. Rats in NC group and M group were given the same volume of normal saline for 8 weeks. The body mass and blood sugar level of rats in each group were measured. High density lipoprotein cholesterol (HDL-C), low density lipoprotein cholesterol (LDL-C), urea nitrogen bun (BUNA), urinary microalbumin (UAlb), urinary creatinine (Cr), serum creatinine (SCR), TCU, triglyceride (TGG), uric acid UAA (UAA), glutathione (GSH), malondialdehyde (MDA) were detected in each group. The levels of superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) activity were observed by HE staining. Results: compared with NC group, the body weight of rats in group M was decreased, and the levels of TG and MDA in UAlb / UCr-SCrU were increased. The levels of HDL-C and GSH were lower than those of control group. The activities of SOD and GSH-Px were decreased (P0.05) and the activity of GSH-Px was decreased (P 0.05). Compared with the M group, the body weight of MM and MH groups increased P0.05A, and the levels of TG and MDA of UAlb UCr-USCr-USCRU were decreased. The levels of HDL-C and GSH were increased. The activities of P0.05C and GSH-Px were increased (P0.05). Compared with NC group, the pathological changes of kidney tissue, glomerular congestion and renal tubule edema were observed in group M, the pathological changes of renal tissue in group M were better than those in group M, and the degeneration of renal tubule vacuolation was alleviated. Interstitial hyperplasia was not obvious. ConclusionDECB combined with metformin can reduce blood sugar, regulate blood lipid, improve renal histopathological changes, alleviate renal injury and improve antioxidant ability of diabetic rats.
【作者单位】：北华大学药学院微生物与生化药学教研室;
【基金】：吉林省科技厅科技创新与科技成果转化计划项目资助课题(0150312014ZX);吉林省科技厅医药产业发展基金资助课题(20140311084YY) 吉林省发改委科研基金资助课题(2014Y103) 吉林省卫计委科研基金资助课题(20142078)
【分类号】：R587.2;R692.9

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