氧化应激-炎性因子对氟中毒卵巢早衰的影响

发布时间：2018-05-25 13:52

本文选题：燃煤型氟中毒 + 氧化应激　；参考：《现代预防医学》2017年12期

【摘要】：目的探讨氧化应激-炎性因子在燃煤型氟中毒致雌鼠卵巢早衰机制。方法模拟贵州燃煤污染型疫区,煤烘烤玉米为雌鼠饮食氟饲料。体重均等,24只清洁级SD雌性大鼠构建动物模型,随机分为对照组、低氟组(25 mg/Kg)、中氟组(50 mg/Kg)、高氟组(100 mg/Kg)。染氟90 d处死雌鼠,分离双侧卵巢。检测致卵巢早衰氧化应激和炎性因子,谷胱甘肽过氧化物酶(GSH-Px)、总抗氧化能力(T-AOC)、活性氧簇(ROS)活性和白细胞介素6(IL-6)、白细胞介素8(IL-8)含量;透射电镜观察卵巢早衰超微结构变化。结果各模型组与对照组比较,GSH-Px、T-AOC、ROS和IL-6、IL-8均有统计学意义(P0.05)。各模型组间比较,随染氟剂量的增加,卵巢GSH-Px、T-AOC逐渐降低,ROS和IL-6、IL-8逐渐增高,差异均有统计学意义(P0.05)。透射电镜下,低氟组卵巢颗粒细胞超微结构电镜结果未见明显损伤。中氟组:颗粒细胞核膜轻微皱缩,少部分细胞核染色质聚集、边集,核仁消失,线粒体轻微肿胀。间质细胞核膜轻微皱缩,核染色质边集,呈现早衰现象。高氟组:颗粒细胞的核固缩,大量染色质成块状,边集,核仁消失,大量的线粒体肿胀,脂滴增多,呈现早衰现象,与染氟剂量呈正相关。结论燃煤型氟中毒卵巢氧化应激水平和炎性机制发生紊乱,抗氧化GSH-Px、T-AOC明显降低,促氧化ROS和促炎性因子IL-6、IL-8明显增高,卵巢超微结构的明显损伤,呈现早衰现象,与染氟剂量呈正相关。
[Abstract]:Objective to investigate the mechanism of oxidative stress-inflammatory factors in premature ovarian failure induced by coal-burning fluorosis in female rats. Methods Coal roasted corn was used as dietary fluorine feed for female rats in Guizhou coal-burning polluted epidemic area. Twenty-four clean female SD rats were randomly divided into control group, low fluorine group (25 mg / kg), middle fluoride group (50 mg / kg), high fluoride group (100 mg / kg). Female rats were killed on 90 d after fluoride exposure and bilateral ovaries were isolated. The activities of oxidative stress and inflammatory factors, glutathione peroxidase (GSH-PxX), total antioxidant capacity (T-AOCN), reactive oxygen species (Ros), interleukin-6 (IL-6) and interleukin-8IL-8 (IL-8) were measured, and the ultrastructural changes of premature ovarian failure were observed by transmission electron microscope (TEM). Results compared with the control group, the GSH-PxX T-AOCU Ros and IL-6 IL-8 in the model group were significantly higher than those in the control group (P 0.05). Compared with each model group, with the increase of fluoride dose, the level of GSH-PxX T-AOC in ovary decreased gradually, Ros and IL-6 IL-8 increased gradually, and the difference was statistically significant (P 0.05). Under transmission electron microscope, there was no obvious damage to the ultrastructure of ovarian granulosa cells in low fluorine group. In the medium fluoride group, the granular nuclear membrane was slightly shrinked, a few of the nuclear chromatin gathered, edge set, nucleolus disappeared, and mitochondria slightly swollen. The nuclear membrane of mesenchymal cells shrinks slightly and chromatin edge sets, showing premature senescence. In high fluorine group, nuclear pyknosis of granulosa cells, a large number of chromatin in blocks, edge aggregation, disappearance of nucleoli, swelling of mitochondria, increase of lipid droplets, preterm aging, and positive correlation with fluorine concentration. Conclusion the levels of oxidative stress and inflammatory mechanism in the ovary of coal-burning fluorosis were disturbed, and the antioxidant GSH-PxX T-AOC was significantly decreased, the pro-oxidant ROS and the pro-inflammatory factor IL-6 IL-8 were significantly increased, and the ultrastructure of the ovary was obviously damaged, showing premature senescence. There was a positive correlation with the dose of fluoride.
【作者单位】：贵阳中医学院第一附属医院检验科;贵州医科大学附属医院血液内科;贵州省骨科医院药剂科;贵州省人民医院肝胆外科;
【基金】：贵州省科学技术厅项目(黔科合LH字[2016]7511号) 贵州省教育厅青年科技人才成长项目(黔教合KY[2016]190)
【分类号】：R599.1

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