三种眼外肌自身抗体在甲状腺相关性眼病患者血清中的表达及意义

发布时间：2018-05-26 02:56

  本文选题：甲状腺相关性眼病 + 眼外肌　； 参考：《山西医科大学》2015年硕士论文

【摘要】：目的:通过检测抗肌集钙蛋白(calsequestrin)抗体、抗葡萄膜自身抗原(uveal antoantigen with coiled coil domains and ankyrin repeats,UACA)抗体、抗G2s抗体3种自身抗体在甲状腺相关性眼病患者、单纯Graves病患者及健康人群血清中的表达水平,初步探讨这三种自身抗体在甲状腺相关性眼病患者发病中的特点。方法:研究对象分为三组,共90例,其中TAO组为34例(男13例,女21例)、Graves病(GD)组28例(男11例,女17例)和健康对照(CON)组28例(男11例,女17例)。三组研究对象的基本资料经统计学分析,在性别、年龄以及病程间均具备可比性,并签署了知情同意书。分别取外周抗凝血,在离心后提取血浆,选用双抗原夹心法(酶联免疫吸附法),分别测定所有研究对象血清中抗calsequestrin抗体、抗UACA抗体、抗G2s抗体、三类自身抗体的浓度。应用SPSS19.0统计学软件进行分析,每个指标的浓度值以x±s表示,分别对三组数据进行分析,对符合正态性且方差齐的资料数据采用方差分析并进行各组间的LSD多重比较方法。对于资料分布类型呈偏态分布或方差不齐的使用秩和检验中的Kruskal-Wallis法及Bonferroni法进行组间的比较。P0.05为差异具有统计学意义。结果:(1)抗calsequestrin抗体在各组研究对象血清中的浓度(ng/L)分别为:TAO组153.0206±104.84868;GD组99.7002±66.42114;CON组88.0589±39.73018;TAO组与CON组比较,P=0.004;TAO组与GD组比较,P=0.016;GD组与CON组比较,P=0.604。(2)抗UACA抗体在各组研究对象血清中的浓度(ng/L)依次为:TAO组60.5365±38.23955;GD组46.6286±17.99325;CON组34.2016±12.59909;TAO组与CON组比较,P=0.000;TAO组与GD组比较,P=0.075;GD组与CON组比较,P=0.002。(3)抗G2s抗体在在各组研究对象血清中的浓度(m U/L)分别为:TAO组68.7604±35.82627;GD组64.9248±31.29806;CON组62.6828±18.40611;TAO组与CON组比较,P=0.478;TAO组与GD组比较,P=0.654;GD组与CON组比较,P=0.799。结论:(1)经ELISA法检测,抗calsequestrin抗体在TAO患者血清中的浓度明显高于GD患者和健康人群,结果存在统计学差异(P0.05),抗calsequestrin抗体可以作为由免疫介导的TAO患者眼肌损伤的敏感性指标。(2)抗UACA抗体在TAO患者血清中的浓度水平明显超出正常人群,结果存有统计学意义(P0.05),抗UACA抗体可以作为自身免疫病的一种临床标志物,本次实验未发现TAO患者血清中抗UACA抗体的表达与GD患者血清中的表达存在差异。(3)本次实验未发现在TAO患者血清中抗G2s抗体的浓度与GD患者及健康人群存有差异,尚不能认为抗G2s抗体可以作为与TAO有关的一个标记物。
[Abstract]:Objective: to detect three kinds of autoantibodies against calsequestrin, uveal antoantigen with coiled coil domains and ankyrin repeat UACAand G2s antibodies in patients with thyroid associated ophthalmopathy. To explore the characteristics of the three autoantibodies in the pathogenesis of thyroid associated ophthalmopathy. Methods: the subjects were divided into three groups, including 34 cases (male 13 cases, female 21 cases with Graves disease) group (28 cases male 11 cases, female 17 cases) and healthy control group 28 cases (male 11 cases, female 17 cases). The basic data of the three groups were statistically analyzed and compared among sex, age and course of disease, and informed consent was signed. The plasma was extracted from peripheral anticoagulant blood after centrifugation. The concentrations of anti calsequestrin antibody, anti UACA antibody, anti G 2s antibody and three kinds of autoantibodies were determined by double antigen sandwich assay (Elisa). Three groups of data were analyzed by using SPSS19.0 software, and the concentration of each index was expressed as x 卤s. The data with normality and homogeneity were analyzed by ANOVA and LSD multiple comparison among each group. For the data distribution type of skewed distribution or variance of the use of rank sum test in the Kruskal-Wallis and Bonferroni methods to compare between groups. P0.05 was statistically significant difference. Results the concentration of calsequestrin antibody in the serum of each group was 153.0206 卤104.84868 g / L respectively, 99.7002 卤66.42114Con group 88.0589 卤39.73018 TAO group compared with GD group and GD group compared with GD group. 渚濇�′福�

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