Graves病患者外周血Th17细胞与肠道中特定菌群的检测

发布时间：2018-05-26 03:32

本文选题：Graves病 + 肠道菌群　；参考：《兰州大学》2017年硕士论文

【摘要】：目的:通过研究Graves病(graves diseases,GD)患者外周血中辅助性T细胞(helper T cell)Th17细胞频率以及其肠道菌群中普雷沃氏菌(Prevotella Shan and Collins)与分节丝状菌(Segmented Filamentous Bacteria,SFB)与正常人群组含量的差别,探讨两种细菌与该自身免疫性甲状腺疾病发生与进展的关系。方法:收集30例初发Graves患者的新鲜粪便及外周血作为GD组,30例健康对照者新鲜粪便样品及外周血作为NC组作为研究样本,即分为患者组与健康对照组。用试剂盒提取粪便中肠道菌群的总DNA,分别针对两种细菌即普雷沃氏菌和分节丝状菌,设计特异性引物,通过荧光定量PCR(Real-time PCR)技术对目的基因进行扩增,对比两组两种细菌的含量差异。采用流式细胞技术分析外周血中的Th17细胞进行分析,检测其百分率,对比两组外周血中Th17细胞比例的差异。结果:初发Graves患者组(GD组)的Th17细胞比例明显高于健康对照人群(NC组)(12.46+3.14 vs.7.05+2.12,P0.05),GD组患者外周血Th17细胞百分率与血清中的FT3,FT4呈显著正相关性(r=0.682,r=0.567,P均0.05),与其他甲状腺功能指标无统计学差异(P0.05)。GD组患者肠道菌群中SFB含量约为NC组的2.384×103倍(RQ值为2.384×103),GD组肠道菌群中普雷沃氏菌含量约为NC组的1.825×103倍(RQ值为1.825×103)。结论:1.Graves病患者外周血中Th17细胞比例显著高于正常对照组,且与外周血FT3、FT4呈显著正相关关系;2.Graves患者肠道菌群中分节丝状菌和普雷沃氏菌的含量显著高于正常对照组;
[Abstract]:Objective: To explore the difference between the Th17 cell frequency of auxiliary T cells (helper T cell) in peripheral blood of patients with Graves disease (Graves diseases, GD) and the difference between the contents of Poulet Was bacteria (Prevotella Shan and) and segmental filamentous bacteria in the intestinal microflora and the normal group, and explore the two bacteria and the self. Methods: the relationship between the occurrence and progress of immune thyroid disease. Methods: the fresh feces and peripheral blood of 30 primary Graves patients were collected as group GD. The samples of fresh feces and peripheral blood were used as the research samples in the group of NC, which were divided into the patient group and the healthy control group. The total DNA of the intestinal microflora in the feces was extracted with the test kit. The specific primers were designed for two kinds of bacteria, namely, perveviella and segmental filamentous bacteria. The target genes were amplified by the fluorescence quantitative PCR (Real-time PCR) technique. The difference of the content of the two bacteria in the two groups was compared. The percentage of the Th17 cells in the peripheral blood was analyzed by flow cytometry, and the percentage of the Th17 cells was detected and compared with the Th17 in the peripheral blood. Results: the proportion of cells in the primary Graves group (group GD) was significantly higher than that in the healthy control group (group NC) (group NC) (12.46+3.14 vs.7.05+2.12, P0.05). The percentage of Th17 cells in peripheral blood of the group GD patients was positively correlated with the FT3 and FT4 in the serum (r= 0.682, 0.05), and had no statistical difference from other thyroid function indexes. The SFB content of intestinal microflora in group P0.05.GD was about 2.384 x 103 times (RQ value 2.384 * 103) in group NC, and in group GD, the content of enteric bacteria in intestinal flora was 1.825 x 103 times (1.825 * 103) in NC group (RQ value 1.825 * 103). Conclusion: the ratio of Th17 cells in peripheral blood of patients with 1.Graves disease was significantly higher than that in normal control group, and the FT4 was significantly positive with peripheral blood. The contents of segmented filamentous bacteria and E. coli in 2.Graves patients were significantly higher than those in normal control group.
【学位授予单位】：兰州大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R581.1

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