腺苷酸激活蛋白激酶与肥胖大鼠肝脏脂质沉积的关系

发布时间：2018-05-29 05:43

本文选题：腺苷酸激活蛋白激酶 + 脂质沉积　；参考：《中国老年学杂志》2017年11期

【摘要】：目的探讨肥胖大鼠肝脏组织腺苷酸激活蛋白激酶(AMPK)的表达及其对肝脏脂质沉积的影响。方法选用18只刚断乳的SPF级SD雄性大鼠,随机挑选6只普通饲料喂养为正常组,另一组12只高脂饲料喂养,12 w后成功建立肥胖模型大鼠6只。分别检测体重、Lee指数、内脏脂肪、肝湿重等指标。通过油红O染色和检测肝脏甘油三酯(TG)、总胆固醇(TC)含量评估两组大鼠肝脏脂质沉积的情况。采用Western印迹检测大鼠肝脏磷酸化(p)-AMPK/AMPK、p-ACC/ACC水平。结果与正常组大鼠相比,肥胖组大鼠体重与Lee指数、附睾脂肪、肾周脂肪量、肝湿重、肝脏内TG与TC含量均显著升高,油红O染色结果显示肥胖组肝脏脂质沉积较正常组明显增多。Western印迹结果显示,与正常组大鼠比较,肥胖组大鼠肝脏p-AMPK/AMPK和p-ACC/ACC表达明显降低。两组大鼠肝脏组织中p-AMPK、p-ACC表达水平与肝脏TG、TC呈负相关。结论高脂饮食诱导食源性肥胖大鼠可降低AMPK的活性,从而导致肝脏组织TG、TC合成增加,脂质在肝脏组织中沉积增加。
[Abstract]:Objective to investigate the expression of adenylate activated protein kinase (AMPK) and its effect on lipids deposition in the liver of obese rats. Methods 18 newly weaned SPF grade SD male rats were randomly selected to be fed with normal diet (n = 6). Another group (n = 12) was fed with high fat diet for 12 weeks, and 6 obese rats were successfully established after feeding with high fat diet for 12 weeks. Lee index, visceral fat and wet weight of liver were measured. The lipids deposition in the liver of the two groups was evaluated by oil red O staining and the content of TGG (total cholesterol) in the liver. Western blot was used to detect the level of phosphorylated pAMPK / AMPKP p-ACC-ACC in rat liver. Results compared with the normal group, the body weight and Lee index, epididymal fat, perirenal fat, liver wet weight, TG and TC contents in the liver of obese rats were significantly increased. The results of oil red O staining showed that the lipid deposition in the obese group was significantly higher than that in the normal group. The results of Western blot showed that the expression of p-AMPK/AMPK and p-ACC/ACC in the obese group was significantly lower than that in the normal group. There was a negative correlation between the expression of p-AMPKT p-ACC and TGG TC in the liver of the two groups. Conclusion High fat diet can reduce the activity of AMPK in obese rats, which leads to the increase of TGT-synthesis and the increase of lipid deposition in liver tissue.
【作者单位】：南京中医药大学第二临床医学院;
【基金】：国家自然科学基金(81202742,81403481,81403114)
【分类号】：R589.2

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