乳酸菌对高脂血症大鼠小肠组织形态学变化研究

发布时间：2018-05-30 11:08

本文选题：高脂血症 + 大白鼠　；参考：《内蒙古农业大学》2015年硕士论文

【摘要】：本实验的开展,主要有以下几个目的：第一,研究动物机体在高脂血症条件下动物的机体血脂水平的变化；第二,通过高脂血症动物模型,研究动物小肠组织结构变化,以及使用乳酸菌的预防、治疗,探究其组织结构有何规律性的改变；第三,高脂血症条件下,对比乳酸菌治疗与药物以及饮食调节实验动物肠管组织结构变化,为合理治疗高脂血症提供一定理论依据。本次实验的实验动物为3-4月龄SD大鼠,雌雄参半,通过饲喂高脂饲料建立SD大鼠高脂血症模型,检测建模成功后将其随机分为10组,分别是：空白组、高脂组、普通饲料组、药物组、预防1组、预防2组、预防3组、治疗1组、治疗2组、治疗3组。按照相应的实验方法处理,采样进行肠管各段的组织学切片,拍照测量相应数据。采用Graph Pad Prism 5 Tow way-ANOVA Ordinary软件进行数据统计分析。数据采用均值(x)±标准差(SD)表示。结果以P0.05表示统计学意义结果,组间差异用LSD检验。实验结果显示：1.本实验成功建立了SD大鼠的高脂血症模型；2.建模后不同时间段SD大鼠小肠组织学变化如下：30d和60d的十二指肠、空肠、回肠肠绒毛高度高于90d,隐窝深度浅于90d,黏膜层厚度、内环肌和外纵肌层厚度厚于90d；30d和60d的肠绒毛高度、隐窝深度、黏膜层厚度、内环肌和外纵肌层厚度相差不大；3.建模后相同时期不同实验组SD大鼠小肠组织学变化如下：十二指肠、空肠、回肠的绒毛高度空白组最高,其次依次是药物组、预防1组、普通饲料组,其余预防组和治疗组和高脂组相差不大。十二指肠、空肠、回肠的隐窝深度空白组最浅,其次依次是药物组、预防1组、普通饲料组,其余预防组和治疗组和高脂组相差不大。十二指肠、空肠、回肠的黏膜层厚度、内环肌和外纵肌层厚度中空白组最厚,其次依次是药物组、预防1组、普通饲料组,其余预防组和治疗组和高脂组相差不大。
[Abstract]:The main objectives of this experiment are as follows: first, to study the changes of blood lipid levels in animals with hyperlipidemia; second, to study the changes of tissue structure of small intestine by hyperlipidemia animal model. And the prevention and treatment of lactic acid bacteria, to explore the regularity of its tissue structure changes; third, under hyperlipidemia conditions, compared with the treatment of lactic acid bacteria and drugs, as well as diet regulation of experimental animals intestinal tissue structure changes, To provide a theoretical basis for the rational treatment of hyperlipidemia. The experimental animals in this experiment were SD rats of 3-4 months old, male and female were mixed. SD rats were fed with high fat diet to establish hyperlipidemia model, and were randomly divided into 10 groups: blank group, hyperlipidemia group, common diet group, and normal diet group, after successful detection and modeling, the SD rats were randomly divided into 10 groups, which were: blank group, hyperlipidemia group, and normal diet group. Drug group, prevention group, treatment group and treatment group. According to the corresponding experimental method, the histological sections of each segment of the intestinal tube were sampled and the corresponding data were taken and measured. The data were analyzed by Graph Pad Prism 5 Tow way-ANOVA Ordinary software. The data are expressed by mean value (x) 卤standard deviation (SDD). Results the results were statistically significant with P0.05, and the differences between groups were tested by LSD. The result of the experiment shows that 1: 1. The hyperlipidemia model of SD rats was established successfully. The histological changes of small intestine in SD rats at different time points were as follows: duodenum, jejunum, ileum villi were higher than 90 days, crypt depth was lower than 90 days, mucosal layer thickness, thickness of inner ring muscle and outer longitudinal muscle layer were thicker than 90 d and 60 d, intestinal villi height of duodenum, jejunum and ileum were more than 90 days. The depth of recess, the thickness of mucosal layer, the thickness of inner ring muscle and the outer longitudinal muscle layer were similar. The changes of intestinal histology of SD rats in the same period after modeling were as follows: duodenum, jejunum, ileum, villi height blank group was the highest, followed by drug group, prevention group 1, common feed group, and the following were the following. There was no significant difference between the other prevention and treatment groups and the high fat group. The depth blank group of duodenum jejunum and ileum was the shallowest, followed by drug group, prevention group 1, general feed group, other prevention group and treatment group and high fat group. The mucosal thickness of duodenum, jejunum and ileum, the thickness of inner ring muscle and external longitudinal muscle layer were the thickest in blank group, followed by drug group, prevention group 1, general diet group, other prevention group and treatment group and high fat group.
【学位授予单位】：内蒙古农业大学
【学位级别】：硕士
【学位授予年份】：2015
【分类号】：R589.2

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