miR-93及miR-21在糖尿病视网膜病变患者中的表达及其临床价值

发布时间：2018-06-01 01:04

  本文选题：糖尿病视网膜病变 + mi　； 参考：《眼科新进展》2017年12期

【摘要】：目的探讨血浆微小核糖核酸(micro RNA,mi R)-93及mi R-21在糖尿病视网膜病变(diabetic retinopathy,DR)患者中的表达及其临床价值。方法采用实时荧光定量PCR检测98例DR患者、40例2型糖尿病(type 2 diabetes mellitus,DM)患者和40名健康体检者(对照组)血浆中mi R-93及mi R-21的表达水平。应用ROC曲线评价血浆mi R-93、mi R-21及两项联合对DR的诊断价值,多因素logistic回归分析DR发生的危险因素。Pearson相关分析DR患者血浆mi R-93、mi R-21与各生化指标的相关性。结果 DR组和DM组的尿白蛋白/肌酐、糖化血红蛋白及空腹血糖均明显高于对照组(均为P0.05),且DR组均明显高于DM组(均为P0.05)。DR组血浆miR-93及miR-21表达水平(1.84±0.27、1.95±0.34)均明显高于DM组(1.09±0.13、1.13±0.18)和对照组(1.02±0.08、1.08±0.16),差异均有统计学意义(均为P0.05)。PDR组血浆miR-93及miR-21表达水平(2.31±0.57、2.28±0.61)均明显高于NPDR组(1.40±0.21、1.52±0.24),差异均有统计学意义(均为P0.05)。血浆miR-93、miR-21及两项联合诊断DR的曲线下面积(95%CI)分别为0.892(0.824~0.961)、0.836(0.770~0.905)、0.937(0.868~0.992)。尿白蛋白/肌酐、糖化血红蛋白、空腹血糖、miR-93及miR-21是DR发生的的独立危险因素。DR患者血浆miR-93、miR-21与尿白蛋白/肌酐、糖化血红蛋白、空腹血糖均呈正相关(均为P0.01),且miR-93与miR-21呈正相关(P0.01)。结论血浆miR-93和miR-21与DR的发生发展密切相关,可作为DR诊断的生物学标志物。
[Abstract]:Objective to investigate the expression and clinical value of plasma microRNAi micro RNA-93 and miR-21 in patients with diabetic retinopathy. Methods the expression of miR-93 and miR-21 in plasma of 98 patients with Dr and 40 patients with type 2 diabetes mellitus (type 2 diabetes mellitus) and 40 healthy controls (control group) were detected by real-time fluorescence quantitative PCR. Results urinary albumin / creatinine was measured in Dr and DM groups. The levels of plasma miR-93 and miR-21 in Dr group were significantly higher than those in DM group (1.84 卤0.27 卤1.95 卤0.34) and 1.09 卤0.13 卤0.18 in DM group and 1.02 卤0.081.08 卤0.16 in control group, respectively. The expression levels of miR-93 and miR-21 in P0.05).PDR group were significantly higher than those in NPDR group (2.31 卤0.572.28 卤0.61) and 1.40 卤0.21 卤1.52 卤0.24 in NPDR group (all P 0.05). There was a positive correlation between fasting blood glucose (P 0.01) and miR-93 and miR-21 (P 0.01). Conclusion Plasma miR-93 and miR-21 are closely related to the occurrence and development of Dr, and can be used as biomarkers in the diagnosis of Dr.
【作者单位】： 海南西部中心医院眼科;
【分类号】：R587.2;R774.1
，

本文编号：1962193