地塞米松喷雾剂联合牙髓干细胞治疗糖尿病Beagle犬种植体周围炎的实验研究

发布时间：2018-06-03 08:03

  本文选题：地塞米松喷雾剂 + 牙髓干细胞　； 参考：《中国临床药理学杂志》2017年23期

【摘要】：目的探索地塞米松喷雾剂联合牙髓干细胞治疗糖尿病(DM)Beagle犬种植体周围炎对骨吸收和炎症因子的影响。方法 8只健康成年Beagle犬用四氧嘧啶诱导法构建DM Beagle犬模型,分离培养牙髓干细胞,构建DM Beagle犬种植体周围炎模型。将8只Beagle犬随机分为对照组、实验A组、实验B组及实验C组,每组2只。对照组予以0.9%Na Cl每次2 m L,tid,喷雾+Bio-Oss骨粉+Bio-Gide膜覆盖处理;实验A,B,C组在对照组治疗的基础上,分别将0.9%NaCl改为地塞米松喷雾剂、牙髓干细胞悬液、地塞米松喷雾剂+牙髓干细胞悬液,其余处理均相同。4组Beagle犬均治疗24周。用X线片观察术后第24周近、远中边缘骨高度的变化,用酶联免疫吸附(ELISA)法检测龈沟液的肿瘤坏死因子-α(TNF-α)及白细胞介素-6(IL-6)的表达。结果术后第24周,对照组、实验A组、实验B组和实验C组的近中边缘骨高度较术后的变化分别为(-0.42±0.14),(-0.36±0.12),(-0.29±0.11)和(-0.17±0.07)mm,远中边缘骨高度较术后的变化分别为(-0.30±0.12),(-0.26±0.14),(-0.21±0.11)和(-0.12±0.09)mm,TNF-α分别为(82.72±19.31),(68.26±14.60),(54.78±14.43)和(42.51±12.95)ng·L~(-1),IL-6分别为(36.20±8.14),(31.32±9.36),(26.85±6.61)和(20.48±5.24)ng·L~(-1),实验C组的上述指标和对照组比较,差异均有统计学意义(均P0.05)。结论地塞米松喷雾剂联合牙髓干细胞治疗可有效地降低DM Beagle犬种植体周围炎的炎症因子水平及骨吸收。
[Abstract]:Objective to investigate the effects of dexamethasone spray combined with dental pulp stem cells on bone resorption and inflammatory factors in diabetic DMC Beagle dogs. Methods eight healthy adult Beagle dogs were induced by alloxan to construct DM Beagle canine model. Dental pulp stem cells were isolated and cultured, and the model of peri-implant inflammation of DM Beagle dog was established. Eight Beagle dogs were randomly divided into control group, experimental group A, experimental group B and experimental group C, with 2 dogs in each group. The control group was treated with 0.9%Na Cl 2 mL / time and sprayed with Bio-Gide membrane of Bio-Oss bone powder, and the experimental group of 0.9%NaCl was changed into dexamethasone spray and dental pulp stem cell suspension on the basis of the control group. Dexamethasone spray dental pulp stem cell suspension, other treatments were the same as 4 groups of Beagle dogs were treated for 24 weeks. The changes of bone height in the proximal and distal margin of gingival effusion were observed by X-ray film. The expression of TNF- 伪 (TNF- 伪) and interleukin-6 (IL-6) in gingival crevicular fluid were detected by enzyme linked immunosorbent assay (Elisa). Results 24 weeks after operation, control group, experimental group A, 瀹為獙B缁勫拰瀹為獙C缁勭殑杩戜腑杈圭紭楠ㄩ珮搴﹁緝鏈�鍚庣殑鍙樺寲鍒嗗埆涓�(-0.42卤0.14),(-0.36卤0.12),(-0.29卤0.11)鍜，

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