176例HIV抗体不确定结果的带型分析

发布时间：2018-06-04 02:22

本文选题：蛋白印迹试验 + 快速检测试验　；参考：《中国艾滋病性病》2017年01期

【摘要】：目的通过蛋白印迹试验(WB)中艾滋病病毒(HIV)抗体不确定条带的特点,分析WB试验存在的问题及解决措施。方法对2010-2015年确证试验中出现的176例不确定样本的WB带型,以及随访到的55例样本再次确证的WB带型进行比对分析。结果 176例不确定样本中,来源于中心血站的占66.5%(117例),医疗机构占25.0%(44例),自愿咨询检测者占7.4%(13例),羁押人员占0.1%(2例)。不确定结果条带有20种带型,不确定带型居前三位的是:p24(+)占46.6%(82例);gp160(+)占12.5%(22例),p17(+)p24(+)占8.0%(14例)。核心蛋白(Gag)类的类型有2种,样本例数构成比54.5%(96例);外模蛋白(Env)类的带型有13种,样本例数构成比41.5%(73例),多聚酶(Pol)类的带型有5种,样本例数构成比为4.0%(7例)。完成随访的55例样本中,Env类条带的阳转率为55.6%(15/27),Gag类条带的阳转率为18.5%(5/27),Pol类条带的阳转率为0(0/1)。Gag类的不确定结果带型以p24居多,多与非特异性反应有关,但是不可忽视早期感染的可能。结论出现Env类带型的不确定结果阳转率较高,感染HIV的可能性更大;终末期病例应加强核酸检测,以免不确定样本流失。
[Abstract]:Objective to analyze the characteristics of HIV / AIDS antibody uncertain bands in Western blot test (WB), and analyze the existing problems and solutions of WB test. Methods the WB band patterns of 176 uncertain samples and 55 follow-up samples from 2010 to 2015 were compared and analyzed. Results among 176 uncertain samples, 117 cases came from the central blood station, 44 cases from medical institutions, 7.4 cases from voluntary counseling, and 2 cases from detainees. There were 20 bands in the result strip of uncertainty, and the top three of them were:% p24 (), 46.6%, 82 cases, gp160 (), 12.5A, 22 cases, p17 (p24 () accounting for 8.0%, 14 cases. There are 2 types of core protein Gag-type, 54.5% of sample number, 13 kinds of outer mode-protein Env-type, 41.5% of 7cases, and 5 kinds of polymerase Pol-type, and the ratio of sample number to sample number is 4.0%. In 55 cases of follow-up, the positive conversion rate of the Env band was 55.60.The positive conversion rate of the 15 / 27 / 27 Gag band was 18.5%. The positive conversion rate of the Pol band was the most uncertain result band type of 0(0/1).Gag, which was mostly related to the nonspecific reaction, but the possibility of early infection should not be ignored. Conclusion the positive rate of Env band type is higher and the possibility of HIV infection is higher. In the end stage cases, nucleic acid detection should be strengthened to avoid the loss of uncertain samples.
【作者单位】：唐山市疾病预防控制中心;唐山市曹妃甸区医院;
【分类号】：R512.91

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