利拉鲁肽对糖尿病模型大鼠肾脏组织核因子-κB、单核细胞趋化蛋白-1和血管细胞间黏附分子-1表达的影响

发布时间：2018-06-05 05:07

本文选题：利拉鲁肽 + 糖尿病　；参考：《中国老年学杂志》2017年09期

【摘要】：目的探讨利拉鲁肽对糖尿病(DM)大鼠肾脏组织核因子(NF)-κB、单核细胞趋化蛋白(MCP)~(-1)和血管细胞间黏附分子(VCAM)~(-1)表达的影响。方法选用8周龄雄性SD大鼠54只,随机分为正常对照组、模型组、胰岛素对照组和利拉鲁肽低、中、高剂量组(100、200和300μg·kg~(-1)·d~(-1)),采用高脂高糖饲料喂养大鼠4 w后,再给予链脲佐菌素(STZ)35 mg/kg腹腔注射建立动物模型。药物干预8 w后测定血糖、血肌酐和尿素氮等生化指标改变。采用免疫组织化学SP方法和病理图像定量分析技术检测大鼠肾组织NF-κB、MCP~(-1)和VCAM~(-1)的表达水平。结果与模型组相比,胰岛素对照组及利拉鲁肽各剂量组大鼠血肌酐、血尿素氮、尿白蛋白/肌酐均明显降低(P0.01);与模型组相比,利拉鲁肽各剂量组肾组织NF-κB、MCP~(-1)和VCAM~(-1)的表达降低(P0.05)。结论利拉鲁肽通过下调DM大鼠肾脏组织NF-κB、MCP~(-1)和VCAM~(-1)表达,发挥对肾小球毛细血管内皮细胞的保护作用,从而改善DM大鼠肾损伤。
[Abstract]:Objective to investigate the effects of liraropeptide on the expression of NF-魏 B, monocyte chemoattractant protein (MCP- 1) and vascular intercellular adhesion molecule (VCAMU-1) in the kidney of rats with diabetes mellitus (DMN). Methods Fifty-four 8-week-old male Sprague-Dawley rats were randomly divided into normal control group, model group, insulin control group and low, medium and high dose groups of 100200 and 300 渭 g kg 路kg ~ (-1) 路d ~ (-1), respectively. The rats were fed with high-fat and high-sugar diet for 4 weeks. The animal model was established by intraperitoneal injection of streptozotocin (STZ) for 35 mg/kg. Blood glucose, serum creatinine and urea nitrogen were measured after 8 weeks of intervention. Immunohistochemical SP method and pathological image quantitative analysis technique were used to detect the expression of NF- 魏 B (MCP-1) and VCAMN (-1) in rat renal tissue. Results compared with the model group, the levels of serum creatinine, blood urea nitrogen and urinary albumin / creatinine were significantly decreased in the insulin control group and the various dosage groups of Lilarudine, compared with the model group, the expression of NF- 魏 B- MCPCP-1 and VCAM- 1) in the renal tissue of each dose group decreased P0.05. Conclusion Lilaru peptide can protect glomerular capillary endothelial cells by down-regulating the expression of NF- 魏 BnMCP-1) and VCAMP-1 in renal tissue of DM rats, thereby improving renal injury in DM rats.
【作者单位】：齐齐哈尔医学院临床病理诊断中心;齐齐哈尔医学院附属第二医院内分泌科;
【基金】：齐齐哈尔市科技计划项目(No.SFGG-201524) 齐齐哈尔医学院院内科研基金项目(QY2016M-04)
【分类号】：R587.2

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