糖尿病肾病大鼠铁超载对内皮功能及氧化应激的影响

发布时间：2018-06-05 14:22

本文选题：铁超载 + 糖尿病肾病　；参考：《中国老年学杂志》2017年22期

【摘要】：目的探讨糖尿病肾病(DN)大鼠铁超载后对内皮功能及氧化应激的影响。方法将采用随机数字法30只健康雄性Wistar大鼠分为正常对照组(NC组)10只和糖尿病模型组20只,糖尿病模型组按65 mg/kg体重单次腹腔注射链脲佐菌素。将20只造模成功的大鼠仍按随机数字法分为DN组10只和铁超载组(Fe组)10只。NC组和DN组继续给予普通全价颗粒饲料,Fe组给予高铁饲料,喂养12 w。实验结束前1 d收集24 h尿液测量尿白蛋白,计算尿白蛋白排泄率(UAER);心脏采血检测血清铁(SI)、总铁结合力(TIBC)、组织铁(TI)、内皮素(ET)-1、血肌酐(Scr)、尿素氮(BUN)等相关指标;观察肾脏及肝脏病理情况;制备肾组织匀浆检测一氧化氮(NO)、一氧化氮合酶(NOS)、超氧化物歧化酶(T-SOD)及丙二醛(MDA)。结果 Fe组大鼠的肾重/体重(KW/BW)显著高于DN组(P0.05)。Fe组血糖、UAER、Scr、BUN、SI、TI显著高于DN组(P0.01),TIBC显著低于DN组(P0.01);肾组织NO、NOS显著低于DN组,ET-1显著高于DN组(P0.01);T-SOD显著低于DN组(P0.01),MDA显著高于DN组(P0.01)。结论铁超载能促进DN的发生与发展,机制可能与血糖水平升高、内皮功能障碍及氧化应激有关。
[Abstract]:Objective to investigate the effect of iron overload on endothelial function and oxidative stress in diabetic nephropathy (DN) rats. Methods 30 healthy male Wistar rats were divided into normal control group (group NC) and 10 rats with diabetic model group (20 rats). The diabetic model group was injected with streptozotocin at a single dose of 65 mg/kg, and 20 models were successfully made. The rats were still divided into DN group and 10 group of iron overload group (Fe group) and 10 group of iron overload group (Fe group) and group DN continued to give ordinary full price particle feed. Group Fe was given high iron feed, 24 h urine albumin excretion rate (UAER) was collected before the end of 12 W. experiment, and urine albumin excretion rate (UAER); blood serum iron (SI), total iron binding force (TIBC). Tissue (TI), endothelin (ET) -1, serum creatinine (Scr), urea nitrogen (BUN) and other related indexes, and the pathological conditions of kidney and liver were observed, and renal tissue homogenate was prepared to detect nitric oxide (NO), nitric oxide synthase (NOS), superoxide dismutase (T-SOD) and malondialdehyde (MDA). The renal weight / weight (KW/BW) of the Fe group was significantly higher than that of the DN group. Sugar, UAER, Scr, BUN, SI, TI were significantly higher than those in group DN (P0.01), TIBC was significantly lower than that of DN group (P0.01), and the renal tissue was significantly lower than that of the group. It is related to chemical stress.
【作者单位】：内蒙古民族大学临床医学院;河南省确山县人民医院;
【基金】：内蒙古自治区教育厅基金资助项目(NJZY11205)
【分类号】：R587.2;R692.9

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