百草枯对星形胶质细胞中非递质类物质水平的影响

发布时间：2018-06-06 23:47

本文选题：百草枯 + 星形胶质细胞　；参考：《毒理学杂志》2017年02期

【摘要】：目的探讨百草枯(paraquat,PQ)对星形胶质细胞合成与释放的非递质物质水平的影响。方法人星形胶质(HA1800)细胞分别暴露于终浓度为0、200、400和800μmol/L的PQ 12、24和48 h,ELISA试剂盒法检测细胞培养上清液中胶质源性神经营养因子(GDNF)和脑源性神经营养因子(BDNF)水平。胆固醇定量检测试剂盒法和Western Blot法分别检测0和400μmol/L的PQ对细胞膜中胆固醇含量和载脂蛋白E(Apo E)表达水平的变化。结果与相应时点的阴性对照组比较,PQ能显著降低细胞膜中胆固醇含量和Apo E表达水平(P0.05),PQ也能以剂量依赖方式降低细胞培养上清液中GDNF和BDNF水平(P0.05)。结论 PQ能通过影响星形胶质细胞非递质类物质水平,参与调控突触发生及可塑性,进而参与神经退行性疾病的发生。
[Abstract]:Objective to investigate the effect of paraquat PQ on the synthesis and release of non-neurotransmitters from astrocytes. Methods the levels of glial derived neurotrophic factor (GDNF) and brain-derived neurotrophic factor (BDNF) in the supernatant of cultured human astrocytes were detected by Elisa kit with final concentrations of 0200400 and 800 渭 mol/L PQ1224 and 48 h, respectively. Cholesterol quantitative assay kit and Western Blot assay were used to detect the changes of cholesterol content and apolipoprotein E(Apo E expression in the cell membrane of 0 and 400 渭 mol/L PQ, respectively. Results compared with the negative control group at the corresponding time points, PQ could significantly decrease the level of cholesterol and Apo E expression in the cell membrane. P0.05PQ could also decrease the levels of GDNF and BDNF in the supernatant of cell culture in a dose-dependent manner. Conclusion PQ may be involved in the regulation of synaptogenesis and plasticity by affecting the level of non-neurotransmitter substances in astrocytes and thus participate in the occurrence of neurodegenerative diseases.
【作者单位】：哈尔滨医科大学公共卫生学院;黑龙江省疾病预防控制中心公共卫生监测所;
【基金】：哈尔滨医科大学于维汉院士杰出青年培养基金
【分类号】：R595.4

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