高剂量热休克蛋白gp96通过激活调节性T细胞预防1型糖尿病

发布时间：2018-06-10 01:22

  本文选题：gp + Tregs　； 参考：《生物工程学报》2016年12期

【摘要】：旨在以非肥胖糖尿病(Non-obese diabetic,NOD)小鼠为动物模型,研究高剂量昆虫细胞表达的重组热休克蛋白gp96(Recombinant gp96,rgp96)对1型糖尿病(Type 1 diabetes,T1D)的预防作用。以高剂量rgp96免疫NOD小鼠,用血糖仪监测小鼠血糖值,用流式细胞术检测小鼠脾脏CD4~+CD25~+Foxp3~+调节性T细胞(Regulatory T cells,Tregs)亚群频率,然后用一系列体外实验探究高剂量rgp96对Tregs的影响。结果显示高剂量rgp96免疫有效地预防或延缓小鼠T1D发病,免疫诱导Tregs数量明显增加。体外实验发现rgp96蛋白促进Tregs增殖,诱导Foxp3表达上调和IL-10分泌增加。研究结果为开发基于rgp96的新型T1D预防和治疗性疫苗提供了依据。
[Abstract]:To study the preventive effect of recombinant heat shock protein gp96 (Recombinant gp96 rgp96) on type 1 diabetes mellitus (T1D) mice with non-obesity-related diabetes mellitus (NOD) as an animal model, and to study the preventive effects of recombinant heat shock protein gp96 / Recombinant gp96 / rgp96 on type 1 diabetes mellitus (type 1 diabetes). High dose rgp96 was used to immunize nod mice. Blood glucose levels were monitored by blood glucose analyzer. The frequency of T cells Tregs subsets in spleen of mice was detected by flow cytometry. The effects of high dose rgp96 on Tregs were investigated by a series of experiments in vitro. The results showed that high dose of rgp96 could effectively prevent or delay the onset of T1D in mice and increase the number of Tregs induced by T1D. It was found in vitro that rgp96 protein promoted the proliferation of Tregs and induced the up-regulation of Foxp3 expression and the increase of IL-10 secretion. The results provide a basis for the development of a novel T 1D preventive and therapeutic vaccine based on rgp96.
【作者单位】： 中国科学院微生物研究所中国科学院病原微生物与免疫学重点实验室;
【基金】：国家自然科学基金(Nos.31230026,81321063,81471960) 深圳市科技创新委员会项目(Nos.JSGG20140516112337659,CYZZ20130826112642412)资助~~
【分类号】：R587.1
，

本文编号：2001484