氟调控成骨细胞内质网应激信号通路对细胞增殖凋亡分化的影响

发布时间：2018-06-10 07:04

本文选题：氟 + 成骨细胞　；参考：《中国地方病防治杂志》2017年03期

【摘要】：目的探讨氟调控成骨细胞内质网应激信号通路对细胞增殖凋亡分化的影响。方法培养成骨细胞MC3T3-E1,0、1、2、4、8、16、32、64 mg F-/L组9个不同的氟浓度处理成骨细胞MC3T3-E1 2、7、14 d后,每个时间段设置对照组,CCK8检测细胞的增殖;2、8、20 mg F-/L处理细胞,流式细胞仪检测不同浓度氟对成骨细胞MC3T3-E1凋亡的影响;qRT-PCR检测细胞中与分化相关的基因的表达;Western blot检测内质网应激信号通路相关蛋白的表达。结果低浓度氟(2、4mg F-/L)和中浓度氟(8 mg F-/L)显著增强细胞的增殖(P0.05),高浓度氟(16 mg F-/L)显著降低细胞的增殖(P0.05);2、8、20 mg F-/L处理成骨细胞MC3T3-E1,细胞凋亡率显著高于0 mg F-/L(P0.01),且随着浓度的增加凋亡率变大;2 mg F-/L促进细胞内ALP、OCN、Runx2和Osterix的mRNA表达,20 mg F-/L则抑制上述因子的mRNA表达(P0.01);2、8、20 mg F-/L处理MC3T3-E1细胞中Xbp1、AFT4、Bip和PERK的蛋白表达量都高于0 mg F-/L,其中2 mg F-/L处理细胞中Xbp1和AFT4蛋白表达最多(P0.01),8 mg F-/L处理细胞中PERK的蛋白表达量最多(P0.01),20 mg F-/L处理细胞中Bip蛋白表达量最多(P0.01)。结论低浓度氟促进成骨细胞MC3T3-E1增殖和分化,高浓度则抑制,细胞凋亡率随氟浓度增加而增加,不同氟浓度处理促进成骨细胞中内质网应激信号通路相关蛋白的表达。
[Abstract]:Objective to investigate the effect of fluoride on proliferation, apoptosis and differentiation of osteoblast endoplasmic reticulum (ER) stress signaling pathway. Methods Osteoblast MC3T3-E1OOOOOOOVEROVICAL cells were treated with 9 different concentrations of fluorine for 714 days. The proliferation of MC3T3-E1 cells was assayed by CCK8 at each time period, and the proliferation of MC3T3-E1 cells treated with F-R / L was detected by CCK8 at each time period, and the proliferation of MC3T3-E1 / L cells was measured at each time interval after treatment with 9 different concentrations of fluorine, and the proliferation of MC3T3-E1 / L cells was measured. Effects of different concentrations of fluoride on apoptosis of osteoblasts MC3T3-E1 the expression of differentiation-related genes in osteoblasts was detected by qRT-PCR and the expression of ERS-related proteins was detected by Western blot. Results the cell proliferation was significantly enhanced by 4 mg F / L and 8 mg F / L at low and middle concentrations, and the proliferation of MC3T3-E1 was significantly decreased by high concentration of F / L (16 mg F / L). The apoptosis rate of MC3T3-E1 cells was significantly higher than that of MC3T3-E1 treated with 820 mg F-P / L of high concentration of fluorine. The apoptosis rate of MC3T3-E1 cells was significantly higher than that of MC3T3-E1 cells with concentration of 0 mg F-P / L, and the apoptosis rate of MC3T3-E1 cells was significantly higher than that of MC3T3-E1 cells. Increased apoptosis rate increased by 2 mg F / L and enhanced mRNA expression of Osterix and Osterix mRNA in MC3T3-E1 cells treated with 2 mg F / L and 20 mg F-P / L, the protein expression of Xbp1AFT4Bip and Perk in MC3T3-E1 cells was significantly higher than that in MC3T3-E1 cells treated with 2 mg F-r-L, and the protein expression of Xbp1AFT4Bip and PERK in MC3T3-E1 cells was significantly higher than that in MC3T3-E1 cells treated with 2 mg F-rL. Xbp1 and AFT4 proteins were most expressed in the cells treated with P0.01mg / L and P0.01mg / L, and the highest expression of Bip protein was found in the cells treated with P0.01mg / L and 20 mg / L P0.01mg / L respectively, and the highest expression of Bip protein was found in the cells treated with P0.01mg / L and P0.01mg / L respectively. Conclusion low concentration of fluoride can promote the proliferation and differentiation of osteoblasts MC3T3-E1, and high concentration of fluoride can inhibit the proliferation and differentiation of MC3T3-E1. The apoptosis rate of MC3T3-E1 cells increases with the increase of fluoride concentration. Different fluoride concentrations can promote the expression of ER stress signaling pathway related protein in osteoblasts.
【作者单位】：齐齐哈尔医学院附属第三医院骨科;
【基金】：齐齐哈尔市科学技术计划项目(SFZD-2014068)
【分类号】：R599.1

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