梓醇对成骨细胞-破骨细胞共育体系中破骨细胞活性、凋亡的影响及其机制研究

发布时间：2018-06-10 09:27

本文选题：梓醇 + 成骨细胞　；参考：《中国药房》2016年10期

【摘要】：目的:研究中药单体梓醇在成骨细胞(OB)-破骨细胞(OC)共育体系中对OC活性、凋亡的影响及其作用机制。方法:分别选用1~3 d龄和5~7 d龄SD乳鼠分离培养OB和OC,建立OB-OC共育体系。通过倒置显微镜观察0(空白对照)、0.05、0.5、5、50、100 mg/L梓醇培养48、72、96 h后OC的骨吸收陷窝数目,以反映OC活性;以0(空白对照)、0.05 mg/L梓醇培养细胞48、72、96h,检测OC中抗酒石酸酸性磷酸酶(TRACP)活性,计算OC的凋亡率;以0(空白对照)、0.05 mg/L梓醇培养细胞并检测OB中骨保护素(OPG)m RNA的表达。结果:在OB-OC共育体系中,0.05~50 mg/L梓醇作用下所形成骨吸收陷窝数目显著低于空白对照(P0.01),表明梓醇可抑制OC活性,其中质量浓度为0.05 mg/L时作用最明显。与空白对照比较,0.05 mg/L梓醇作用后OC中TRACP的活性降低、OC的凋亡率升高(P0.05);OB的OPG m RNA表达上调(P0.01)。结论:在OB-OC共育体系中,梓醇可抑制OC活性、诱导OC凋亡,其机制可能与上调OB的OPG m RNA表达有关。
[Abstract]:Aim: to study the effect and mechanism of catalpol on OC activity and apoptosis in osteoblast OBM-osteoclast co culture system. Methods: OB and OC were isolated and cultured from SD rats aged 3 days and 57 days, respectively, and the co-culture system of OB-OC was established. The number of bone resorption lacunae of OC was observed by inverted microscope, and the activity of OC was measured by 0. 05 mg / L catalpol 50 mg / L catalpol culture for 48 ~ 722 ~ 96 h, and the activity of tartrate-resistant acid phosphatase TRACPP was detected by 0 (0. 05 mg / L catalpol culture for 48? 722? 96 h). The apoptosis rate of OC was calculated and the expression of osteoprotegerin (OPG) mRNA in OB was detected by 0 (control group) 0.05 mg / L catalpol. Results: in OB-OC co-culture system, the number of bone resorption lacunae formed under the action of 0.05 mg / L catalpol was significantly lower than that of the control group (P0.01), indicating that catalpol could inhibit OC activity, especially when the mass concentration was 0.05 mg / L. Compared with the control group, the activity of TRACP in OC decreased after treatment with 0.05 mg / L catalpol. The apoptosis rate of OC increased. The expression of OPG mRNA of P0.05 and OB upregulated P0.01. Conclusion: catalpol can inhibit OC activity and induce OC apoptosis in OB-OC co-culture system. The mechanism may be related to the up-regulation of OB OPG mRNA expression.
【作者单位】：广东食品药品职业学院;暨南大学药学院;
【基金】：广东省医学科学技术研究基金项目(No.B2015063) 广东食品药品职业学院自然科学基金项目(No.2014YZ003)
【分类号】：R580

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