CILP2基因表达上对小鼠糖代谢的影响

发布时间：2018-06-13 08:07

本文选题：胰岛素钳夹术 + CILP2　；参考：《重庆医科大学》2015年硕士论文

【摘要】：目的：探讨CILP2基因表达上调对小鼠糖代谢的影响。方法：将48只C57BL/6J小鼠随机均分为6组：普通饲料+生理盐水组(NCN组,n=8)、普通饲料+空载病毒组(NCG组,n=8)、普通饲料+CILP2组(NCC组,n=8)、高脂饲料+生理盐水组(HCN组,n=8)、高脂饲料+空载病毒组(HCG组,n=8)、高脂饲料+CILP2组(HCC组,n=8)。采用葡萄糖耐量试验(GTT)及扩展胰岛素钳夹技术评价糖代谢和胰岛素敏感性的变化。结果：普食喂养条件下,各项指标均无统计学差异。高脂喂养条件下,HCC组血浆胰岛素(FIns)、游离脂肪酸(FFA)、甘油三酯(TG)以及总胆固醇(TC)水平均相较于于HCN和HCG组明显升高(P0.05或P0.01)。在相同的高胰岛素输注下,HCC组葡萄糖输注率(GIR)相较于HCN、HCG组明显降低(均P0.01)。而HCC组的肝糖输出率(HGP)则分别高于HCN、HCG组,仅被抑制30%和32%(均P0.05),葡萄糖清除率(GRd)相较于于HC、HCG组显著降低(P0.05)。结论：CILP2表达上调使胰岛素的抗脂解作用进一步减弱,加重了外周和肝脏的胰岛素抵抗。
[Abstract]:Objective: to investigate the effect of up-regulation of CILP2 gene expression on glucose metabolism in mice. Methods: Forty-eight C57BL / 6J mice were randomly divided into 6 groups: normal saline group, NCN group, NCG group, CILP2 group, HCN group and high fat diet group, respectively. The HCG group and the high fat feed CILP2 group were treated with HCG group and HCC group respectively, and the HCG group with HCG group and the HCC group with high fat diet (CILP2 group). Glucose tolerance test (GTT) and extended insulin clamp technique were used to evaluate the changes of glucose metabolism and insulin sensitivity. Results: under the condition of general feeding, there was no statistical difference in all indexes. Compared with HCN and HCG groups, the plasma levels of insulin, FInsn, FFAA, TGand TCC in HCN and HCG groups were significantly higher than those in HCN and HCG groups (P 0.05 or P 0.01). Compared with HCN-HCG group, the glucose infusion rate of HCC group was significantly lower than that of HCN-HCG group (all P 0.01). However, the HGPs of HCC group were significantly higher than those of HCG group, and were only inhibited by 30% and 32% (both P0.05N, glucose clearance rate GRD) compared with HCG group, significantly lower than HCG group. Conclusion the up-regulation of the expression of CILP2 further weakens the antilipidolysis of insulin and exacerbates the insulin resistance in the peripheral and liver.
【学位授予单位】：重庆医科大学
【学位级别】：硕士
【学位授予年份】：2015
【分类号】：R587.1

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