UCP1基因单核苷酸多态性位点与成人超重肥胖的关系

发布时间：2018-06-18 19:15

本文选题：基因 + 超重　；参考：《中华疾病控制杂志》2017年03期

【摘要】：目的研究解偶联蛋白1(uncoupling protein 1,UCP1)基因SNPs位点与超重肥胖的关系。方法采用连接酶检测技术对SNPs进行基因分型,检测275例超重肥胖者与253例正常体重者UCP1基因4个SNP位点的基因型,利用SNP Stats在线软件构建单倍体型,并分析单倍体型与性别及非遗传因素的交互作用。结果两组间体重、体质指数、总胆固醇、甘油三酯、高密度脂蛋白、低密度脂蛋白差异有统计学意义(均有P0.05);隐性遗传模式下,rs1800592位点基因型(AA+AG)与GG在两组间差异有统计学意义(P=0.048,OR=1.154,95%CI:1.002~2.286));与单倍体型CTAA相比,CTCA、CTAG、TACG在两组间分布差异有统计学意义(CTCA:P=0.043,OR=0.658,95%CI:0.439~0.988;CTAG:P=0.043,OR=0.658,95%CI:0.439~0.988;TACG:P=0.004,OR=0.449,95%CI:0.257~0.786);单倍体型与血清总胆固醇之间存在交互作用(χ~2=15.955,P=0.014);与血清总胆固醇正常组单倍体型CTAA相比,在血清总胆固醇异常组,单倍体型CTAA、CTCG、CTCA、CTAG在两组间的分布差异有统计学意义(CTAA:P0.001,OR=2.731,95%CI:1.550~4.752;CTCG:P0.001,OR=9.768,95%CI:3.403~28.042;CTCA:P=0.037,OR=2.713,95%CI:1.082~7.159;CTAG:P=0.037,OR=2.713,95%CI:1.028~7.159)。结论隐性遗传模式下,基因型(AA+AG)可能是超重肥胖的危险因素;单倍体型CTCG、CTCA、TACG可能是超重肥胖的保护因素;血清总胆固醇异常时,单倍体型CTAA、CTCG、CTCA、CTAG可能是超重肥胖的危险因素。
[Abstract]:Objective to study the relationship between uncoupling protein 1(uncoupling protein 1 (UCP1) gene SNPs and overweight and obesity. Methods the genotypes of SNPs in 275 overweight and 253 normal body weight subjects were detected by ligase assay. Haplotypes were constructed by SNP Stats online software, and 4 SNP loci of UCP1 gene were genotyped in 275 overweight and 253 normal weight subjects. The interaction of haploid type with sex and non-genetic factors was analyzed. Results body weight, body mass index, total cholesterol, triglyceride, high density lipoprotein between the two groups, The difference of low density lipoprotein (LDL-C) was statistically significant (all P0.05; genotype AA at locus rs1800592) and GG in recessive genetic model. There was significant difference between the two groups in the distribution of CTCAGTACG between the two groups. The distribution of CTCAGTACG was statistically different from that of the haploid type CTAA in the two groups (P < 0.05), and the distribution of CTCAGTACG was significantly different from that of the haploid type CTAA (P < 0.05), and the distribution of CTAGTACG between the two groups was statistically different from that of the haploid type CTAA (P < 0.05). CTCA: P0. 043: 0. 658 / 95CI: 0. 439 / 0. 988 CTAG: P0. 043: 0. 65895 CIQ: 0. 659: 0. 3988TACG-: 0.004: 0. 449 / 95 CIW 0.2570.786. there is an interaction between haploid and total serum cholesterol (蠂 2 / 2, 15.955P0. 01414); compared with the haploid type of CTAA in the normal group of total cholesterol, In the group with abnormal serum total cholesterol, there were significant differences in the distribution of CTCATAG between the two groups. There was significant difference between the two groups in the distribution of CTAA: P0.001OR.95CI1.550 and 4.752CTCAP0. 76895 CIV: 3.40328.042CTCAPU 0.037: 2.71395CIW 1.082 / 7.159CTAG / P0.037.37. The CIQ was 1.02828 / 7.1596.28 / 7 / 7 ~ (-1) ~ (th) ~ (?) ~ _ _ _ Conclusion under the recessive genetic model, genotype AA AGG may be the risk factor of overweight and obesity, haploid CTCG CTCATACG may be the protective factor of overweight and obesity, and haploid type CTAACTCGCGCTCATAG may be the risk factor of overweight and obesity when serum total cholesterol is abnormal.
【作者单位】：郑州大学公共卫生学院卫生统计学教研室;郑州大学基础医学院组织学与胚胎学教研室;
【基金】：国家自然科学基金(81001280,81202277,81373096) 河南省高等学校重点科研资助项目(16A330003)
【分类号】：R589.2

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