锰中毒大鼠海马锌含量与锌转运蛋白的关系

发布时间：2018-06-20 12:08

本文选题：锰中毒 + 海马　；参考：《环境与职业医学》2017年08期

【摘要】：[目的]探讨锰中毒大鼠海马区锰和锌含量变化及与锌转运蛋白3(ZnT3)之间的关系。[方法]将40只雄性SD大鼠随机分成4组,每组10只,即生理盐水对照组和染锰低剂量、中剂量、高剂量组。分别给予腹腔注射生理盐水及氯化锰溶液3、6、12 mg/(kg·d)连续注射8周,锰中毒造模成功后取大鼠海马组织,做HE染色病理切片观察其病理改变,石墨炉原子吸收仪测定海马锰、锌含量变化,Western blot法检测大鼠海马区ZnT3含量,实时荧光定量PCR法测定大鼠海马区ZnT3 mRNA的表达。[结果]染毒后大鼠海马神经细胞均出现不同程度嗜酸性变,胞浆凝聚、结构不清,且出现细胞皱缩和嗜酸性小体。与对照组及低剂量组比较,高、中剂量组大鼠海马组织中锰及锌含量均明显增高(P0.05);而高剂量组锌含量也高于中剂量组(P0.01)。高、中、低剂量组大鼠海马组织中ZnT3含量分别为1.01±0.03、0.80±0.02、0.65±0.13,均高于对照组(0.51±0.06)(P0.05)。高、中剂量组ZnT3基因相对表达分别为3.40±0.36、2.88±0.69,均高于对照组及低剂量组(1.47±0.60)(P0.001)。[结论]锰中毒可破坏大鼠海马神经元细胞结构,且海马组织中锌含量与ZnT3同步增高。
[Abstract]:[objective] to investigate the changes of manganese and zinc content in hippocampus of rats with manganese poisoning and their relationship with zinc transporter 3 ZnT 3. [methods] Forty male Sprague-Dawley rats were randomly divided into 4 groups, 10 rats in each group: saline control group, low dose, middle dose and high dose group. Rats were injected intraperitoneally with normal saline and manganese chloride solution for 8 weeks. The hippocampal tissues of rats were taken out after the model was established successfully. The pathological changes were observed by HE staining, and the hippocampal manganese was determined by graphite furnace atomic absorption spectrometry (GFAAS). The content of ZnT3 in hippocampus of rats was detected by Western blot and the expression of ZnT3 mRNA in hippocampus of rats was detected by real-time fluorescence quantitative polymerase chain reaction. [results] all the hippocampal neurons showed eosinophilic changes, cytoplasmic condensation, unclear structure, cell shrinkage and eosinophilic bodies. Compared with the control group and the low dose group, the content of mn and Zn in the hippocampus of the middle dose group was significantly higher than that of the control group and the low dose group, while the content of zinc in the high dose group was higher than that in the middle dose group, and the content of zinc in the middle dose group was also higher than that in the middle dose group. The contents of ZnT3 in hippocampus of rats in high, medium and low dose groups were 1.01 卤0.03, 0.80 卤0.02 and 0.65 卤0.13, respectively, which were higher than those in control group (0.51 卤0.06). The relative expression of ZnT3 gene in the high and middle dose groups was 3.40 卤0.36 ~ 2.88 卤0.69, respectively, which was higher than that in the control group and low dose group (1.47 卤0.60). [conclusion] Manganese poisoning can destroy the structure of hippocampal neurons in rats, and the content of zinc in hippocampal tissue increases synchronously with ZnT3.
【作者单位】：遵义医学院公共卫生学院卫生毒理学教研室;
【基金】：贵州省科学技术厅国际科技合作项目(编号:黔科合外G字[2014]7012号)
【分类号】：R595.2

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