不同剂量维甲酸诱导骨质疏松大鼠模型的稳定性

发布时间：2018-06-21 16:07

本文选题：骨质疏松 + 维甲酸　；参考：《中国组织工程研究》2017年20期

【摘要】：背景:骨质疏松严重危害老年人的健康和生活质量,建立理想的实验动物模型对骨质疏松的病因和治疗的研究有着重要的意义。目的:通过不同剂量的维甲酸诱导建立大鼠骨质疏松模型,筛选出理想的造模剂量。方法:将80只雌性SD大鼠按体质量随机分为对照组、低剂量组、中剂量组和高剂量组,各20只。低剂量组、中剂量组和高剂量组分别以80,100,120 mg/(kg·d)维甲酸连续灌胃14 d。结果与结论:与对照组相比,低剂量组大鼠骨密度、成骨细胞和破骨细胞计数及骨微观结构无明显变化,而中、高剂量组大鼠股骨骨密度、血清钙含量明显下降,股骨破骨细胞数量增加,骨微观结构明显发生改变,其中以中剂量组最为明显。结果说明100 mg/(kg·d)维甲酸灌胃14 d可诱导大鼠建立骨质疏松模型,且稳定性良好。
[Abstract]:Background: osteoporosis is a serious hazard to the health and quality of life of the elderly. It is of great significance to establish an ideal experimental animal model for the study of the etiology and treatment of osteoporosis. Aim: to establish rat model of osteoporosis by different doses of retinoic acid, and to screen out the ideal dose. Methods: 80 female SD rats were randomly divided into control group, low dose group, middle dose group and high dose group. Low, middle and high dose groups were given retinoic acid at 80100120 mg/(kg d for 14 d. Results and conclusion: compared with the control group, the bone mineral density (BMD), osteoblast and osteoclast count and bone microstructure in the low dose group were not significantly changed, while the bone mineral density and serum calcium content in the middle and high dose groups were significantly decreased. The number of osteoclasts of femur increased and the microstructure of bone changed obviously, especially in middle dose group. The results showed that mg/(kg d) retinoic acid could induce osteoporosis model in rats for 14 d and had good stability.
【作者单位】：广州中医药大学第一附属医院创伤骨科;广州市正骨医院骨科;广州中医药大学;
【基金】：广东省科技计划项目(2014A020212593)~~
【分类号】：R-332;R580

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