持续性与间歇性低载荷机械振动对成骨细胞增殖与分化的影响

发布时间：2018-06-23 06:13

本文选题：持续性低载荷机械振动 + 间歇性低载荷振动　；参考：《中国老年学杂志》2017年09期

【摘要】：目的观察不同低载荷振动方式对成骨细胞增殖与分化的影响。方法应用振动加载器给予培养的成骨细胞MC3T3-E1以强度为0.25 g、频率35 Hz的振动载荷,设置空白组(A组),持续振动10 min(B组)及振动5 min间歇5 min再振动5 min(C组)两种加载模式组,加载结束后分别检测各组MC3T3-E1细胞的增殖能力(CCK-8法)、碱性磷酸酶(ALP)活性及Runx-2蛋白的表达变化。结果 3组之间成骨细胞增殖情况无明显统计学意义(P=0.742),B组与C组ALP活性及Runx-2蛋白的表达明显高于A组,C组的ALP活性及Runx-2蛋白的表达显著高于B组(P0.05)。结论低载荷机械振动对成骨细胞的增殖并无影响,然而低载荷机械振动可以促进成骨细胞的分化,特别是适当的间歇低载荷机械振动能够更加明显的促进成骨细胞的分化。
[Abstract]:Objective to observe the effects of different low-load vibration modes on the proliferation and differentiation of osteoblasts. Methods Osteoblast MC3T3-E1 was loaded with vibration load of 0.25 g and 35 Hz. The normal group (group A), the group of 10 min continuous vibration (group B) and the group C (group C) were subjected to continuous vibration for 10 min and 5 min interval for 5 min and then vibrated again for 5 min (group C). After loading, the proliferation ability of MC3T3-E1 cells (CCK-8 method), alkaline phosphatase (ALP) activity and the expression of Runx-2 protein were detected. Results there was no significant difference in osteoblast proliferation among the three groups (Pn0.742). ALP activity and Runx-2 protein expression in group B and C were significantly higher than those in group A and C (P0.05). Conclusion Low-load mechanical vibration has no effect on the proliferation of osteoblasts, but low-load mechanical vibration can promote the differentiation of osteoblasts, especially the intermittent low-load mechanical vibration can obviously promote the differentiation of osteoblasts.
【作者单位】：吉林大学白求恩第一医院;大连医科大学;
【基金】：国家自然科学基金资助(No.11432016,11272134,11602093)
【分类号】：R580

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