硒对氟诱导NRK-52E细胞凋亡干预作用机制

发布时间：2018-06-23 06:31

本文选题：硒 + 氟中毒　；参考：《中国公共卫生》2017年01期

【摘要】：目的探讨硒对氟诱导NRK-52E细胞凋亡的拮抗作用。方法实验设对照组、染氟组、染硒组、硒干预组,培养NRK-52E细胞,染毒72 h,采用流式细胞仪检测细胞凋亡,Western blot检测AMPK及线粒体通路相关蛋白表达水平。结果与对照组[(5.97±0.09)%]比较,5、20 mg/L Na F组细胞凋亡率[分别为(7.92±0.24)%、(11.06±0.17)%]明显升高(P0.05);与20 mg/L Na F组比较,17.1、34.2μg/L硒干预组细胞凋亡率[分别为(8.46±0.09)%、(9.88±0.08)%]明显降低(P0.05);与对照组比较,20 mg/L Na F组细胞AMPK磷酸化蛋白、bax、Cyt-C蛋白表达[分别为(0.73±0.16)、(0.99±0.16)、(0.73±0.08)]、活性caspase-9和caspase-3蛋白表达[分别为(1.17±0.17)、(1.51±0.42)]均升高(P0.05);与20 mg/L Na F组比较,17.1、34.2 g/L硒干预组AM PK磷酸化蛋白表达量[分别为(0.46±0.12)、(0.48±0.15)]、bax、Cyt-C蛋白表达量[分别为(0.55±0.09)、(0.61±0.16)与(0.30±0.06)、(0.34±0.05)]及活性caspase-9、caspase-3蛋白表达量[分别为(0.76±0.11)、(0.40±0.12)与(0.35±0.12)、(0.27±0.04)]均降低(P0.05)。结论 AMPK介导的线粒体通路参与了硒拮抗氟诱导NRK-52E细胞凋亡过程。
[Abstract]:Objective to investigate the antagonistic effect of selenium on fluoride-induced apoptosis of NRK-52 E cells. Methods NRK-52E cells were cultured in control group, fluoride group, selenium group and selenium intervention group for 72 h. AMPK and mitochondrial pathway related protein expression were detected by flow cytometry (FCM). Results compared with the control group [(5.97 卤0.09)%], the apoptotic rate [(7.92 卤0.24), (11.06 卤0.17)%] in the 20 mg / L NAF group was significantly higher than that in the control group [(5.97 卤0.09)%], and the apoptosis rate in the 20 mg / L NAF group [(8.46 卤0.09), (9.88 卤0.08)%] was significantly lower than that in the 20 mg / L NAF group (P 0.05). The expression of AMPK phosphorylated protein was significantly higher than that of 20 mg / L NAF group [0.73 卤0.16), (0.99 卤0.16), (0.73 卤0.08], active caspase-9 and caspase-3 protein [1.17 卤0.17), (1.51 卤0.42, respectively], and the expression of AM PK phosphorylated protein [0.46 卤0.12), (0.48 卤0.15] in 20 mg / L NAF group [0.46 卤0.12), (0.48 卤0.15] compared with 20 mg / L NAF group [0.46 卤0.12), (0.48 卤0.15] The protein expression of caspase-9), (0.55 卤0.09), (, 0.61 卤0.16) and (0.30 卤0.06), (, 0.34 卤0.05) and active caspase-9 (0.76 卤0.11), (, 0.40 卤0.12) and (0.35 卤0.12), (, 0.27 卤0.04, respectively) were decreased (P0.05). Conclusion the mitochondrial pathway mediated by AMPK is involved in the apoptosis of NRK-52E cells induced by fluoride.
【作者单位】：山西医科大学实验动物中心实验动物与人类疾病动物模型山西省重点实验室;
【基金】：山西省实验动物专项(2012k02) 山西医科大学青年资金项目(02201429)
【分类号】：R599.1

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