一种新的干粉试剂盒检测艾滋病患者CD4细胞绝对计数和百分比的效果

发布时间：2018-06-25 16:53

本文选题：HIV + CD淋巴细胞　；参考：《现代预防医学》2017年18期

【摘要】：目的评价美国Beckman Coulter公司(BC公司)的三色荧光抗体干粉试剂(CD3/CD4/CD8)在流式细胞仪上检测CD4细胞计数及CD4细胞百分比的准确性。方法于2015年12月开始在云南省疾病预防控制中心收集艾滋病自愿咨询检测(VCT)门诊的血液样本和送检样本共计201份,分别用美国的Becton Dickinson公司(BD公司)的液态三色荧光抗体试剂和BC公司的干粉试剂进行样本处理,使用BD FACSCalibur流式细胞仪和Multiset软件程序进行CD4细胞绝对计数和百分比分析,用SPSS软件和Bland-Altman图对2种试剂检测结果的一致性进行分析。结果 BC和BD试剂对201例样本的CD4细胞计数的均值分别为(506.3±605.3)个/μl(10~2 458)和(501.0±610.3)个/μl(7~2 406),Pearson相关系数为0.984,平均偏差为+5.3个/μl(95%CI:-102.3~113.0);CD4细胞百分比的结果具有很好的相关性,Pearson相关系数为0.992,平均偏差为+0.66%(95%CI:-3.6%~4.9%)。以CD4细胞计数等于350个/μl为治疗阈值,BC干粉试剂的敏感性和特异性分别为95.8%和98.4%。结论与BD试剂相比,BC干粉试剂在FACSCalibur流式细胞仪上检测CD4细胞的绝对数和百分比具有较好的相关性和一致性,干粉试剂的保存和运输无需冷链,适合基层单位推广应用。
[Abstract]:Objective to evaluate the accuracy of Beckman Coulter (BC) tricolor fluorescent antibody dry powder reagent (CD3 / CD4 / CD8) in detecting CD4 cell count and percentage of CD4 cells by flow cytometry. Methods in December 2015, 201 blood samples were collected from the outpatient clinic of Voluntary Counseling and testing (VCT) for AIDS at the Centers for Disease Control and Prevention of Yunnan Province. The samples were treated with Becton Dickinson Inc. (BD) liquid tricolor fluorescent antibody reagent and BC company dry powder reagent, respectively. The absolute count and percentage of CD4 cells were analyzed by BD FACSCalibur flow cytometry and Multiset software program. SPSS software and Bland-Altman diagram were used to analyze the consistency of the two reagents. Results the average count of CD4 cells by BC and BD was (506.3 卤605.3) / 渭 l (102 / 458) and (501.0 卤610.3) / 渭 l / 渭 l respectively. Pearson correlation coefficient was 0.984, and the average deviation was 5.3 / 渭 l (95CIv-102.3113.0). Pearson correlation coefficient was very good. The average deviation was 0.66% (95% CI: -3.6%). The sensitivity and specificity of BC dry powder reagent with CD4 cell count equal to 350 / 渭 l were 95.8% and 98.4%, respectively. Conclusion compared with BD reagent, the absolute number and percentage of CD4 cells detected by FACSCalibur flow cytometry have good correlation and consistency. The preservation and transportation of dry powder reagent do not need cold chain, so it is suitable for popularization and application of basic unit.
【作者单位】：云南省疾病预防控制中心性病艾滋病防制所;
【基金】：中美艾滋病防治合作项目(GAP项目)
【分类号】：R512.91

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