1,2-二甲基-3-羟基-4-吡啶酮和牛磺酸联合应用对铝染毒大鼠肾功能及抗氧化系统的保护作用

发布时间：2018-06-28 02:16

本文选题：铝 + DFP　；参考：《环境与健康杂志》2017年02期

【摘要】：目的探讨1,2-二甲基-3-羟基-4-吡啶酮(deferipone,DFP)和牛磺酸联合作用对染铝大鼠肾脏功能、抗氧化系统及ATP酶活力的影响。方法将56只SPF级雄性Wistar大鼠按体重随机分为7组,以灌胃方式染毒。其中,阴性对照组给予1.0 ml/d生理盐水,连续8周。前4周,铝染毒组、牛磺酸干预组、低剂量DFP干预组、高剂量DFP干预组、牛磺酸与低剂量DFP联用组、牛磺酸与高剂量DFP联用组均给予281.40 mg/(kg·d)AlCl_3·6H_2O;后4周,各组分别给予1.0 ml/d生理盐水、400 mg/(kg·d)牛磺酸、13.82 mg/(kg·d)DFP、27.44 mg/(kg·d)DFP、400 mg/(kg·d)牛磺酸+13.82 mg/(kg·d)DFP、400 mg/(kg·d)牛磺酸+27.44 mg/(kg·d)DFP。测定大鼠肾脏Na~+K~+-ATP酶、Mg~(2+)-ATP酶、Ca~(2+)-ATP酶、超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)活力和丙二醛(MDA)含量及肌酐(Cr)、尿素氮(BUN)含量。结果与铝染毒组相比,各干预组大鼠肾脏GSH-Px、Na~+K~+-ATP酶、Mg~(2+)-ATP酶活力均明显升高,肾脏MDA含量和血清BUN含量均明显降低,差异有统计学意义(P0.05);且高剂量DFP干预组及联合用药组大鼠肾脏SOD活力明显升高,差异有统计学意义(P0.05)。与单独用药组相比,联合用药组Na~+K~+-ATP酶、Mg~(2+)-ATP酶、SOD、GSH-Px活力明显升高,MDA和血清BUN含量明显降低,差异有统计学意义(P0.05)。各组间Ca~(2+)-ATP酶活力及血清Cr含量无明显差异(P0.05)。结论在一定剂量范围内,与牛磺酸或DFP单独用药相比,牛磺酸和DFP联合用药可以对染铝大鼠肾脏功能及抗氧化系统起到更好的保护作用。
[Abstract]:Objective to investigate the effects of 1-dimethyl-3-hydroxy-4-pyridinone (DFP) and taurine on renal function, antioxidant system and ATPase activity in aluminized rats. Methods 56 SPF male Wistar rats were randomly divided into 7 groups according to their body weight. The negative control group was given 1. 0 ml/d saline for 8 weeks. In the first 4 weeks, aluminum exposure group, taurine intervention group, low dose DFP intervention group, high dose DFP intervention group, taurine combined with low dose DFP group, taurine combined with high dose DFP group were given 281.40 mg/ (kg d) ALCL 36 H 2O, respectively. Each group was given 1. 0 ml/d normal saline for 400 mg/ (kg d) taurine with 13.82 mg/ (kg d) DFPN 27.44 mg/ (kg d) DFPN 400 mg/ (kg d) taurine 13.82 mg/ (kg d) DFPN 400 mg/ (kg d) 27.44 mg/ (kg d) DFP. The activities of Ca ~ (2) -ATPase, superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), malondialdehyde (MDA), creatinine (Cr) and urea nitrogen (bun) in rat kidney were measured. Results compared with the control group, the activities of GSH-PxPxNa-Na ~ -K ~ -ATPase in the kidney of rats were significantly increased, and the contents of MDA and bun in serum were significantly decreased in each intervention group. The difference was statistically significant (P0.05), and the renal SOD activity in the high dose DFP intervention group and the combined treatment group was significantly higher than that in the control group (P0.05). Compared with the control group, the activity of Na ~ K ~ -ATPase mg ~ (2) -ATPase (Na ~ (2) -ATPase) GSH-Px in the combined treatment group was significantly higher than that in the control group, and the content of MDA and serum bun were significantly decreased (P0.05). There was no significant difference in Ca ~ (2) -ATPase activity and serum Cr content among groups (P0.05). Conclusion compared with taurine or DFP alone, taurine combined with DFP can protect renal function and antioxidant system in aluminized rats in a certain dose range.
【作者单位】：山东大学公共卫生学院卫生检验学系;
【基金】：山东省自然科学基金(ZR2013CM043)
【分类号】：R595.2

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