强直性脊柱炎患者外周血中Dickkopf-1及BMP-2的表达及临床意义

发布时间：2018-06-28 02:56

本文选题：强直性脊柱炎 + DKK-1　；参考：《皖南医学院》2015年硕士论文

【摘要】：目的探讨Dickkopf-1(DKK-1)及骨形态发生蛋白-2(BMP-2)在强直性脊柱炎(AS)患者外周血中的表达及临床意义。方法采用酶联免疫吸附法(ELISA)检测40例AS患者和20例年龄、性别相匹配的健康者血清中DKK-1和BMP-2的表达水平,同时收集AS患者的相关临床资料。结果采用SPSS17.0软件包进行统计学分析。结果1.AS患者血清中DKK-1的表达水平(1162.52±391.70)pg/ml低于健康对照组(2237.98±369.82)pg/ml,BMP-2的表达水平(1022.12±257.84)pg/ml则高于健康对照组(468.30±95.85)pg/ml,且差异具有统计学意义(P0.01)。2.影像学上早期AS患者血清中DKK-1的表达(1541.64±199.41)pg/ml高于晚期患者(909.77±260.02)pg/ml(P0.01);而BMP-2的表达(808.23±120.28)pg/ml低于晚期患者(1164.71±224.16)pg/ml(P0.01);X线上有骨桥形成的AS患者血清中DKK-1的表达(679.37±89.71)pg/ml低于无骨桥形成的AS患者(1369.59±264.86)pg/ml(P0.05);而BMP-2的表达(1198.79±227.50)pg/ml则高于无骨桥形成者(946.40±234.78)pg/ml(P0.01);3.HLA-B27阳性的AS患者与阴性患者之间以及不同性别的AS患者之间血清中DKK-1、BMP-2的表达无明显差异(P0.05)。4.对于不同的炎性指标例如ESR、CRP高于正常的患者与ESR、CRP为正常值患者相比,血清DKK-1、BMP-2的表达水平无明显差异(P0.05)。5.AS患者血清中DKK-1、BMP-2的表达水平与其病程、ESR、CRP、IgG以及BASDAI评分之间均无相关性(P0.05)。6.AS患者血清中DKK-1的表达水平与BMP-2呈显著负相关性(r=-0.731,P0.01)。结论1.AS患者血清中DKK-1与BMP-2水平的变化可能参与其新骨形成。2.AS患者血清中DKK-1、BMP-2的表达水平与AS患者ESR、CRP、IgG以及BASDAI评分之间均无相关性,提示新骨形成与炎症可能为2个独立过程。3.AS患者血清中DKK-1的表达水平与BMP-2呈显著负相关性,提示Wnt和BMP信号通路在成骨过程中的可能存在着协同作用。
[Abstract]:Objective to investigate the expression and clinical significance of Dickkopf-1 (DKK-1) and bone morphogenetic protein-2 (BMP-2) in peripheral blood of patients with ankylosing spondylitis (as). Methods the expression levels of DKK-1 and BMP-2 in serum of 40 patients with as and 20 healthy subjects of age and sex were detected by enzyme linked immunosorbent assay (Elisa). The clinical data of patients with as were collected. Results SPSS 17.0 software package was used for statistical analysis. Results 1. The expression of DKK-1 in serum of as patients was (1162.52 卤391.70) pg/ml lower than that of healthy controls (2237.98 卤369.82) pgml-1 / mBMP-2 and (1022.12 卤257.84) pg/ml was higher than that of healthy controls (468.30 卤95.85) PG / ml, and the difference was statistically significant (P0.01) .2. The expression of DKK-1 in early as patients was (1541.64 卤199.41) pg/ml higher than that in advanced patients (909.77 卤260.02) pg/ml (P0.01), while the expression of BMP-2 in (808.23 卤120.28) pg/ml was lower than that in patients with bone bridge formation (1164.71 卤224.16) pg/ml (P0.01). The expression of DKK-1 was (679.37 卤89.71) pg/ml lower than that in patients without bone bridge formation (1369.59 卤264.86) pg/ml (P0.05). The expression of DKK-1mBMP-2 in serum of patients without bone bridge formation (946.40 卤234.78) pg/ml (P0.01) was higher than that of patients without bone bridge (946.40 卤234.78) pg/ml (P0.01). 3. There was no significant difference in the expression of DKK-1mBMP-2 in serum between as patients with HLA-B27 positive and negative patients and patients of different sexes (P0.05). Patients with different inflammatory markers such as ESR-CRP were higher than those with normal ESR-CRP, compared with patients with normal ESR-CRP. There was no significant correlation between the expression level of DKK-1 and BMP-2 in patients with as (P0.05). There was no significant correlation between the expression of DKK-1BMP-2 and the ESR-CRPU IgG and BASDAI score (P0.05). 6. There was a significant negative correlation between the expression of DKK-1 and BMP-2 in as patients (r-0.731p0.01). Conclusion 1. The changes of serum DKK-1 and BMP-2 levels in patients with as may be involved in the expression of DKK-1BMP 2. There is no correlation between the expression of DKK 1 and BMP-2 in serum of as patients and the ESRK CRPU IgG and BASDAI scores. 2. The results suggest that the expression of DKK-1 in serum of patients with as may be negatively correlated with BMP-2, suggesting that Wnt and BMP signaling pathway may play a synergistic role in osteogenesis. 3.
【学位授予单位】：皖南医学院
【学位级别】：硕士
【学位授予年份】：2015
【分类号】：R593.23

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