CCR5第二胞外环的拮抗短肽对哮喘小鼠肺组织炎症细胞浸润和TNF-α表达的影响

发布时间：2018-06-28 03:01

本文选题：CC趋化因子受体 + 拮抗短肽　；参考：《中国病理生理杂志》2017年04期

【摘要】：目的:研究与CC趋化因子受体5(CC chemokine receptor 5,CCR5)第二胞外环特异性结合的拮抗短肽对哮喘小鼠肺组织炎症细胞浸润和TNF-α表达的影响。方法:筛选最适宜的卵白蛋白(ovalbumin,OVA)致敏浓度,构建OVA诱导的BALB/c小鼠哮喘模型。模型构建成功后通过尾静脉注射不同浓度拮抗短肽干预模型小鼠,HE染色对肺组织进行病理细胞学分析及炎症分级,real-time PCR与Western blot实验分别检测小鼠肺组织TNF-α的mRNA和蛋白表达水平。结果:筛选出的OVA最佳致敏浓度为500 mg/L(0.1 mL)。尾静脉注射0.2m L不同浓度(1.5 g/L、2.5 g/L和3.5 g/L)拮抗短肽干预哮喘小鼠,能减轻哮喘小鼠肺组织炎症程度,抑制TNF-α的表达,以2.5 g/L浓度效果最佳,较模型组炎症程度减轻2级,炎症细胞数明显减少,TNF-α的mRNA表达量较模型组下降近90%,蛋白表达水平下降约70%。同时该短肽(2.5 g/L)也起到了一定的预防作用,炎症程度改善1级,TNF-α的mRNA及蛋白水平较模型组下降约50%左右。结论:CCR5第二胞外环的拮抗短肽能有效减轻哮喘小鼠肺组织炎症程度,抑制TNF-α的表达。
[Abstract]:Aim: to study the effects of antagonistic short peptide binding to the second extracellular ring of CC chemokine receptor 5 (CC chemokine receptor 5) on inflammatory cell infiltration and expression of TNF- 伪 in lung tissue of asthmatic mice. Methods: the optimal concentration of ovalbumin OVA was selected to construct the BALB / c mouse asthma model induced by OVA. After the successful construction of the model, the lung tissue was stained by HE staining with different concentrations of antagonistic short peptide. The expression of TNF- 伪 mRNA and protein in the lung tissue was detected by real-time PCR and Western blot respectively. Results: the best sensitizing concentration of OVA was 500 mg / L (0.1 mL). Different concentrations of 0.2m L (1.5 g / L, 2.5 g / L and 3.5 g / L) antagonized short peptide in asthma mice, which could reduce the degree of lung inflammation and inhibit the expression of TNF- 伪 in asthmatic mice. The concentration of 2.5 g / L was the best, and the degree of inflammation in model group was reduced by 2 grades. Compared with the model group, the expression of TNF- 伪 mRNA in the inflammatory cells decreased by 90%, and the protein expression level decreased about 70%. At the same time, the short peptide (2.5 g / L) also played a preventive role. The level of TNF- 伪 mRNA and protein decreased by about 50% compared with the model group. Conclusion the antagonistic peptide of the second extracellular ring of 1: CCR5 can effectively reduce the degree of lung inflammation and inhibit the expression of TNF- 伪 in asthmatic mice.
【作者单位】：中山大学孙逸仙纪念医院儿科;中山大学孙逸仙纪念医院病理科;
【基金】：广东省自然科学基金资助项目(No.2014A030313020;No.2015A030313027;No.2016A03031343)
【分类号】：R562.25

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