SLE患者外周血中IFN-α、IFN-β及OAS1的水平及意义

发布时间：2018-06-29 18:50

  本文选题：干扰素 + OAS1　； 参考：《遵义医学院》2017年硕士论文

【摘要】：目的:通过检测系统性红斑狼疮(Systemic lupus erythematosus,SLE)患者及健康对照组外周血中IFN-α、IFN-β及OAS1的水平,初步探讨SLE患者IFN-α、IFN-β及OAS1的水平与SLE的相关性。方法:分别收集30例SLE患者、30例健康对照者外周静脉血,离心分离血浆,ELISA法分别检测两组IFN-α、IFN-β及OAS1蛋白的水平;分析IFN-α及IFN-β与OAS1之间的相关性;分析血浆中IFN-α、IFN-β及OAS1蛋白水平与系统性红斑狼疮疾病活动性指数(systemic lupus erythematosus disease activity index,SLEDAI)、SLE患者的实验室检测指标及临床症状的相关性。结果:(1)IFN-α、IFN-β及OAS1蛋白的ELISA检测结果:SLE患者组IFN-α、IFN-β及OAS1的水平均较健康对照组显著增氋(369.56±127.69pg/ml vs.171.05±69.43pg/ml,P(27)0.05;1488.78±364.73pg/ml vs.990.22±348.76pg/ml,P(27)0.05;10.87±2.23ng/ml vs.2.27±0.25ng/ml,P(27)0.05)。(2)SLE患者组IFN-α及IFN-β与OAS1的相关性分析结果:IFN-α的水平与OAS1正相关(r=0.439,P=0.015),IFN-β的水平与OAS1无相关性(r=-0.038,P=0.844)。(3)SLE患者组IFN-α、IFN-β及OAS1与SLEDAI的相关性分析结果:IFN-α、OAS1的水平与SLEDAI评分正相关(r=0.534,P=0.002;r=0.505,P=0.004),IFN-β的水平与SLEDAI评分无相关性(r=-0.306,P=0.100)。(4)SLE患者组IFN-α、IFN-β及OAS1与实验室检测指标的相关性分析结果:IFN-α的水平与抗核抗体(ANA)和抗双链DNA抗体(抗ds-DNA抗体)滴度正相关(r=0.7000,P=0.000;r=0.468,P=0.016),与抗Sm抗体等自身抗体无相关(P0.05),血沉升高、补体C3下降阳性组较阴性组IFN-α的水平有显著差异(Z=-2.915,P=0.003;Z=-2.173,P=0.03),白细胞减少等指标阳性组较阴性组IFN-α的水平无差异(P0.05);IFN-β的水平与抗核抗体和自身抗体无相关性(P0.05),且在实验室检测指标阳性组较阴性组的差异无统计学意义(P0.05);OAS1的水平与ANA和抗ds-DNA抗体滴度正相关(r=0.374,P=0.042;r=0.424,P=0.031),OAS1的水平与抗Sm抗体等自身抗体无相关性(P0.05),且在实验室检测指标阳性组和阴性组的差异无统计学意义(P0.05)。(5)SLE患者组IFN-α、IFN-β及OAS1与临床症状的相关性分析结果:肾损害与光过敏阳性组较阴性组IFN-α的水平有差异(Z=-2.318,P=0.020;Z=-2.928,P=0.003),关节炎、发热及颧状红斑等临床症状阳性组较阴性组IFN-α的水平均无差异;肾损害、关节炎等临床症状阳性组较阴性组IFN-β、OAS1的水平均无差异(P0.05)。结论:(1)SLE患者组血浆中IFN-α、IFN-β及OAS1水平较正常组增高,且IFN-α与OAS1的水平正相关,而IFN-β与OAS1水平无相关性,提示OAS1可能受IFN-α的调节从而在SLE发病中发挥作用。(2)SLE组IFN-α和OAS1的水平与SLEDAI评分、ANA及抗ds-DNA抗体滴度正相关,IFN-α的水平与血沉升高、补体C3下降、肾损害、光过敏有关,而IFN-β、OAS1水平与实验室检测指标及临床症状均无关,提示IFN-α及OAS1可能与SLE病情活动性有关,且IFN-α可能参与SLE的病理过程。
[Abstract]:Objective: to investigate the correlation between the levels of IFN- 伪, IFN- 尾 and OAS1 in peripheral blood of patients with systemic lupus erythematosus (SLE) and healthy controls by detecting the levels of IFN- 伪 -IFN- 尾 and OAS1 in peripheral blood of patients with systemic lupus erythematosus (SLE). Methods: peripheral venous blood was collected from 30 patients with SLE and 30 healthy controls. The plasma levels of IFN- 伪 -IFN- 尾 and OAS1 were detected by centrifugation Elisa, and the correlation between IFN- 伪, IFN- 尾 and OAS1 was analyzed. To analyze the correlation between the levels of IFN- 伪 -IFN- 尾 and OAS1 protein and the laboratory indexes and clinical symptoms of SLE patients with systemic lupus erythematosus disease activity index (systemic lupus erythematosus disease activity). 缁撴灉:(1)IFN-伪,IFN-尾鍙奜AS1铔嬬櫧鐨凟LISA妫，

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