类风湿关节炎患者检测血清14-3-3η蛋白和自身抗体的临床意义

发布时间：2018-06-29 18:38

本文选题：类风湿关节炎 + --η蛋白　；参考：《中国免疫学杂志》2017年11期

【摘要】：目的:探讨血清14-3-3η蛋白与自身抗体检测对类风湿关节炎(RA)诊断的临床价值。方法:收集134例RA患者、90例非RA关节病变患者及同期40例健康体检者血清样本,采用ELISA和速率比浊法分别检测14-3-3η蛋白、抗CCP抗体(anti-CCP)、抗RA33抗体(anti-RA33)、抗Sa抗体(anti-Sa)和类风湿因子(RF)含量,并分析其对RA的诊断价值。结果:(1)血清14-3-3η蛋白、anti-CCP、anti-RA33、anti-Sa、RF水平在RA组均显著高于非RA组和对照组,组间差异都具有统计学意义;(2)绘制各指标ROC曲线,14-3-3η蛋白、anti-CCP、anti-RA33、anti-Sa、RF曲线下面积(AUC)分别0.831、0.852、0.615、0.706和0.739,均P0.01;其中14-3-3η蛋白和anti-CCP达到中等以上诊断价值,对应cutoff值分别为2.59 ng/ml和24.10 U/ml;(3)所有指标中anti-Sa特异度最强为97.69%,RF灵敏度最高为83.58%;14-3-3η诊断RA的特异度为91.54%仅稍次于anti-Sa和anti-RA33抗体,但其敏感度较anti-Sa和anti-RA33均高。结论:14-3-3η蛋白、anti-CCP、anti-RA33、anti-Sa和RF在RA中均显著升高,对RA的诊断都有一定的价值。同时,14-3-3η诊断RA的敏感度和特异度相比自身抗体更有一定优势,可以作为辅助诊断RA的指标。
[Abstract]:Objective: to investigate the clinical value of 14-3-3 畏 protein and autoantibody in the diagnosis of rheumatoid arthritis (RA). Methods: the levels of 14-3-3 畏 protein, anti-CCP antibody (anti-CCP), anti-RA33 antibody (anti-RA33), anti-sa antibody (anti-sa) and rheumatoid factor (RF) were detected by Elisa and rate turbidimetry in 134 patients with RA and 40 healthy controls. Its diagnostic value for RA was analyzed. Results: (1) the levels of serum 14-3-3 畏 protein anti-CCPP- anti-RA33 anti-Sag RF in RA group were significantly higher than those in non-RA group and control group. (2) the area (AUC) under the RF curve of ROC curve was 0.831 ~ 0.852n 0.615 ~ (0.706) and 0.739 respectively (P < 0.01), and 14-3-3 畏 protein and anti-CCP were more than moderate diagnostic value. The corresponding cutoff values were 2.59 ng/ml and 24.10 U / ml, respectively. (3) the strongest specificity of anti-Sa was 97.69U / ml. The highest sensitivity of anti-Sa was 83.58%. The specificity of 14-3-3 畏 in diagnosing RA was 91.54%, which was only slightly lower than anti-sa and anti-RA33 antibodies, but its sensitivity was higher than that of anti-sa and anti-RA33. Conclusion both the ratio 14-14-3-3 畏 protein anti-CCPT anti-RA33 anti-Sa and RF are significantly increased in RA, which is valuable in the diagnosis of RA. At the same time, the sensitivity and specificity of 14-3-3 畏 in the diagnosis of RA are more superior than that of autoantibodies, and can be used as an index for the diagnosis of RA.
【作者单位】：江西省医学检验重点实验室南昌大学第二附属医院检验科;
【基金】：江西省科技计划项目(20151122070198、20171BAB205076) 南昌大学研究生创新专项资金
【分类号】：R593.22

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