西格列汀对肥胖大鼠血清及不同组织视黄醇结合蛋白4表达水平的影响

发布时间：2018-07-02 10:24

本文选题：肥胖症 + 胰岛素抵抗　；参考：《安徽医科大学学报》2015年02期

【摘要】：目的研究西格列汀对肥胖大鼠代谢指标、血清视黄醇结合蛋白4(RBP4)及附睾脂肪、肝脏组织RBP4表达水平的影响。方法将40只SD大鼠随机分为对照组(ND组,n=10)和高脂组(HD组,n=30)。16周后HD组成功建立肥胖模型(n=16),并随机分为肥胖对照组(OB组,n=8)、西格列汀干预组(SITA组,n=8)。分别测定干预前后体重(BW)、空腹血糖(FBG)、三酰甘油(TG)、胆固醇(TCH)、高密度脂蛋白(HDL-C)、低密度脂蛋白(LDL-C)、空腹胰岛素(FINS)及血清RBP4水平,并计算胰岛素抵抗指数(HOMAIR)。Western blot法检测大鼠附睾脂肪和肝脏组织RBP4表达水平。结果1与ND组相比,HD组BW、FBG、TG、TCH、LDL-C、FINS、HOMA-IR、RBP4升高,HDL-C降低,差异有统计学意义(P0.05,P0.01)。在HD组中,OB组、SITA组间各指标差异无统计学意义。西格列汀干预后,SITA组较OB组FBG、TG、TCH、LDL-C、FINS、HOMA-IR、RBP4降低,HDL-C升高,差异有统计学意义(P0.05,P0.01)。2与ND组比较,OB组附睾脂肪RBP4蛋白表达水平显著增加(P0.05);与OB组相比,SITA组附睾脂肪RBP4蛋白表达水平显著降低(P0.01)。3 Pearson相关分析显示ND组、血清RBP4与TG呈正相关性;HD组、血清RBP4与FINS、HOMA-IR、TG、附睾脂肪RBP4呈正相关性,与HDL-C呈负相关性。结论肥胖大鼠血清RBP4水平升高,附睾脂肪RBP4表达水平增高。西格列汀干预能显著降低肥胖大鼠血清RBP4水平及附睾脂肪中RBP4蛋白表达水平,并可改善糖脂代谢。
[Abstract]:Objective to study the effects of siglitatin on the metabolic indexes, serum retinol binding protein 4 (RBP4), epididymal fat and the expression of RBP4 in liver of obese rats. Methods Forty Sprague-Dawley rats were randomly divided into control group (ND group) and hyperlipidemia group (HD group (n = 30). After 16 weeks, obesity model was successfully established in HD group (n = 16), and was randomly divided into two groups: obesity control group (OB group) and siglitatin intervention group (SITA group n 8). Body weight (BW), fasting blood glucose (FBG), triglyceride (TG), cholesterol (tch), high density lipoprotein (HDL-C), low density lipoprotein (LDL-C), fasting insulin (fins) and serum RBP4 levels were measured before and after intervention. Insulin resistance index (HOMAIR). Western blot was used to detect the expression of RBP4 in rat epididymal fat and liver. Results 1 compared with ND group, the HDL-C of HD group was significantly higher than that of HD group (P 0.05, P 0.01). In HD group, there was no significant difference between SITA group and OB group. Compared with OB group, the HDL-C level in SITA group was significantly lower than that in OB group, compared with that in OB group. The expression of RBP4 protein in epididymal fat of OB group was significantly higher than that of ND group (P0.05), and the expression level of RBP4 protein in epididymal fat of OB group was significantly lower than that of OB group (P0.01) .3 Pearson correlation analysis showed that the expression level of RBP4 protein in epididymal fat in ND group was significantly lower than that in OB group (P0.01). There was a positive correlation between serum RBP4 and TG, a positive correlation between serum RBP4 and FINSHOMA-IRTG, and a negative correlation between RBP4 and HDL-C in epididymal fat. Conclusion the serum RBP4 level and the epididymal fat RBP4 level in obese rats were increased. The intervention of siglitatin could significantly reduce the level of RBP4 in serum and the expression of RBP4 protein in epididymal fat of obese rats and improve the metabolism of glucose and lipid.
【作者单位】：安徽医科大学第一附属医院内分泌科;安徽中医药大学第一附属医院内分泌科;
【基金】：安徽省高校省级自然科学研究项目(编号:KJ2013Z126) 安徽中医学院中医药管理局重点学科-中医内分泌学科开放基金(编号:2011nfmxk004) 国家自然科学基金青年科学基金培养计划(编号:2011KJ11)
【分类号】：R965

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