白介素-1受体相关激酶-M在系统性红斑狼疮患者单核细胞中的表达及与临床的相关性

发布时间：2018-07-03 03:16

本文选题：系统性红斑狼疮 + 白细胞介素-受体相关激酶-M　；参考：《中国免疫学杂志》2017年02期

【摘要】：目的:研究白介素-1受体相关激酶-M(Interleukin-1 receptor associated kinases M,IRAK-M)在系统性红斑狼疮(Systemic lupus erythematosus,SLE)患者单核细胞中的表达情况及与临床的相关性。方法:采用实时荧光定量PCR技术,检测SLE组和健康对照组单核细胞中IRAK-M的mRNA表达量;采用酶联免疫吸附试验法检测抗双链DNA抗体(ds DNA)和抗单链DNA抗体(ss DNA);采用动态免疫散射比浊法测定补体3(C3)、补体4(C4)和C-反应蛋白(CRP);采用魏氏法测定红细胞沉降率(ESR)。同时,使用Pearson或Spearman分析IRAK-M与SLEDAI评分、ds DNA、ss DNA、C3、C4、CRP和ESR的相关性。结果:1SLE组IRAK-M的mRNA表达量明显低于健康对照组(P0.05),活动期SLE组IRAK-M的mRNA表达量明显低于稳定期SLE组(P0.05)。2SLE组ds DNA、ss DNA、CRP和ESR水平明显高于健康对照组(P0.05),C3和C4水平明显低于健康对照组(P0.05);而且,活动期SLE组ds DNA、ss DNA、CRP和ESR水平明显高于稳定期SLE组(P0.05),C3和C4水平明显低于稳定期SLE组(P0.05)。3相关分析显示:IRAK-M的mRNA表达量与C3成正相关(P0.05),与SLEDAI评分、ds DNA、CRP、ESR呈负相关(P0.05),与ss DNA和C4无相关性(P0.05)。结论:IRAK-M在SLE发病机制中起着重要的作用;定量检测IRAK-M的mRNA表达量可监测SLE疾病活动度和判断预后。同时,对SLE患者ds DNA、ss DNA、CRP、C3、C4和ESR水平的常规检测和定期监测是非常必要的。
[Abstract]:Objective: to investigate the expression of interleukin-1 receptor associated kinases (IL-1 receptor associated kinases) in monocytes of patients with systemic lupus erythematosus (SLE) and its correlation with clinical manifestations. Methods: the expression of IRAK-M mRNA in monocytes of SLE and healthy controls was detected by real-time fluorescent quantitative PCR. Double strand DNA antibody (DS DNA) and anti single strand DNA antibody (ss DNA) were detected by enzyme linked immunosorbent assay (Elisa), complement 3 (C3), complement 4 (C4) and C-reactive protein (CRP) were determined by dynamic immune scattering turbidimetry, and erythrocyte sedimentation rate (ESR) was measured by Wei's method. At the same time, Pearson or Spearman were used to analyze the correlation between IRAK-M and SLEDAI score. Results the expression of IRAK-M mRNA in the SLE group was significantly lower than that in the healthy control group (P0.05). The mRNA expression of IRAK-M in active SLE group was significantly lower than that in the stable SLE group (P0.05). 2 the levels of CRP and ESR in DS DNAss DNA of SLE group were significantly higher than those in the healthy control group (P0.05), and the levels of C _ 3 and C _ 4 in active SLE group were significantly lower than those in the healthy control group (P0.05). The levels of CRP and ESR in active SLE group were significantly higher than those in stable SLE group (P0.05). The levels of C3 and C4 in active SLE group were significantly lower than those in stable SLE group (P0.05). The correlation analysis showed that the mRNA expression of IRAK-M was positively correlated with C3 (P0.05), negatively correlated with SLEDAI score, and had no correlation with SS DNA and C4 (P0.05). Conclusion the expression of IRAK-M mRNA in SLE may monitor the disease activity and predict the prognosis of SLE by quantitatively detecting the mRNA expression of IRAK-M, which plays an important role in the pathogenesis of SLE. At the same time, it is necessary to detect and monitor regularly the levels of C _ 4 and ESR in patients with SLE.
【作者单位】：包头医学院风湿免疫研究所包头医学院第一附属医院内蒙古自治区自体免疫学重点实验室;
【基金】：国家自然科学基金(No.81360464) 内蒙古自治区高等学校科学研究项目(No.NJZY12219)
【分类号】：R593.241

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