IRS2基因Gly1057Asp与环境交互对2型糖尿病易感性的影响

发布时间：2018-07-04 17:22

本文选题：型糖尿病 + 胰岛素受体底物　；参考：《重庆医科大学学报》2016年11期

【摘要】：目的:探究胰岛素受体底物2(insulin receptor substrate 2,IRS2)基因单核苷酸多态性位点Gly1057Asp与环境间的交互作用对2型糖尿病(type 2 diabetes mellitus,T2DM)易感性的影响。方法:对772例无血缘关系的中国重庆汉族人(T2DM 467例,健康对照305例)进行病例-对照研究。应用限制性片段长度多态性聚合酶链反应联合基因测序法检测IRS2基因Gly1057Asp位点基因型,分析其与T2DM的相关性。采用多因子降维法(multifactor dimensionality reduction,MDR)分析Gly1057Asp位点与老龄、肥胖及高甘油三酯血症间的交互作用对T2DM易感性的影响。结果:IRS2基因Gly1057Asp位点与T2DM相关,GG基因型个体T2DM患病风险是AA基因型的1.42倍(OR=1.422,95%CI=1.037~1.949,P=0.029)。MDR分析显示Gly1057Asp位点、年龄、腰围和血浆三酰甘油四个因素构成的交互模型具有统计学意义(P=0.001)。在老龄(年龄60岁)或腹型肥胖者中,Gly1057Asp位点GG或GA基因型个体T2DM患病风险明显增加;在腹型肥胖的高甘油三酯血症者中,除年龄在40~60岁且具AA基因型者外,其余均易患T2DM(OR=3.342,95%CI=1.168~9.561,P=0.026)。结论:同时具有IRS2基因Gly1057Asp位点GG基因型、腹型肥胖和高甘油三酯血症的中国重庆汉族人,可能是T2DM的高危易感人群。
[Abstract]:Objective: To investigate the effect of the interaction between the single nucleotide polymorphism site (Gly1057Asp) of the insulin receptor substrate 2 (insulin receptor substrate 2, IRS2) gene and the environment on type 2 diabetes mellitus (type 2 diabetes mellitus, T2DM) susceptibility. Methods: 772 cases of Chongqing Han people in China (T2DM 467 cases, 305 healthy controls) were carried out. Case control study. The restriction fragment length polymorphism polymerase chain reaction (PCR) combined gene sequencing was used to detect the genotype of IRS2 gene Gly1057Asp, and the correlation with T2DM was analyzed. The multiple factor dimensionality reduction (multifactor dimensionality reduction, MDR) was used to analyze the Gly1057Asp locus with aging, obesity and hypertriglyceridemia. The effect of interaction on T2DM susceptibility. Results: the Gly1057Asp locus of the IRS2 gene is associated with T2DM. The risk of T2DM disease in the GG genotype is 1.42 times the AA genotype (OR=1.422,95%CI=1.037~1.949, P=0.029).MDR analysis shows the Gly1057Asp site, age, waist circumference and plasma three glycerol four factors have statistical meaning. Meaning (P=0.001). Among the elderly (age 60) or abdominal obesity, the risk of T2DM in the Gly1057Asp locus GG or GA genotype individuals increased significantly; among those with high triglyceride in abdominal obesity, the rest were susceptible to T2DM (OR= 3.342,95%CI=1.168~9.561, P=0.026) except for the age of 40~60 and the AA genotypes. Gly1057Asp locus GG genotype, abdominal obesity and hypertriglyceridemia in Han Chinese in Chongqing, China, may be a high-risk susceptible group of T2DM.
【作者单位】：重庆医科大学附属第一医院内分泌科;重庆医科大学感染性疾病分子生物学教育部重点实验室;吉首大学医学院药理教研室与疾病基因研究室;
【基金】：国家临床重点专科建设资助项目(编号:财社[2011]170号) 重庆市卫生局中医药科技课题资助项目(编号:2010-2-134)
【分类号】：R587.1

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