黄芪甲苷对糖尿病大鼠牙周炎的影响与炎症因子间的关系研究

发布时间：2018-07-06 19:40

本文选题：黄芪甲苷 + 糖尿病牙周炎　；参考：《锦州医科大学》2017年硕士论文

【摘要】：目的研究黄芪甲苷(Astragaloside,AS)对糖尿病牙周炎(Diabetic periodontitis,DP)大鼠的影响及其与炎症因子间的相关性,为临床治疗糖尿病牙周炎提供新的药物作用靶点和理论依据。方法采用链脲佐菌素(Streptozotocin,STZ)一次性腹腔注射方法,并协同高糖、高脂食物诱导糖尿病(Diabetic mellitus,DM)大鼠模型,高糖高脂喂养12W后,出现一系列慢性并发症,在此基础上进行上颌磨牙接种牙龈卟啉单胞菌合并结扎丝,丝线结扎引发牙周炎,同时治疗组给予AS灌胃。设置空白对照组(N组),DP模型组(DP组)和AS高(AS-H组)、低剂量治疗组(AS-L组)。对血糖浓度,牙龈炎症表现,牙周袋深度等相关指标进行对比;检测与糖代谢有关的生化指标:糖基化终末产物(Glycosylation end products,AGEs)、C肽、糖化血红蛋白(Glycated hemoglobin,Hb A1C)水平的改变;应用酶联免疫吸附法(ELISA)法检测四组大鼠牙周粘膜中炎症因子的变化,包括测定IL-1、IL-6、TNF-α的表达水平,综合分析AS对DP的作用及其与炎症因子间的关系。结果1.血糖、尿糖等改变:造模成功的DM大鼠空腹血糖均高于16.7mmol/L,明显高于N组,差异有统计学意义(p0.05)。给予AS治疗的高、低剂量组,血糖降低,尿糖呈阴性或弱阳性,两两比较差异具有统计学意义(p0.05)。2.牙周探诊检查AS组牙龈炎症表现减轻,牙周袋变浅、较小附着丧失水平,DP组大鼠重度牙龈炎症表现,牙周袋探诊较深、附着丧失水平较大,N组检查正常,两两比较具有统计学意义(p0.05)。3.大鼠患侧牙周粘膜组织石蜡切片,HE染色显示DP组牙周袋内上皮大量炎症细胞浸润,上颌骨离体样本显示牙槽骨呈水平吸收。AS治疗组显示牙周袋内上皮炎症细胞浸润程度降低,N组无上述变化。4.生化分析仪检测AS治疗组AGEs和Hb A1C水平降低,血清C肽的含量升高,DP组AGEs和Hb A1C水平较高,血清C肽含量低,N组都处于正常水平,两两比较差别有统计学意义(p0.05)。5.酶联免疫吸附法(ELISA法)测定AS治疗组中IL-1、IL-6、TNF-α等炎症因子的水平下降减少,与DP组血清中IL-1、IL-6、TNF-α等炎症因子相比,差异有统计学意义(p0.05)。结论1.AS有降血糖功能,对DP有治疗作用。2.AS可降低DP大鼠的AGEs、Hb A1C的水平,增加C肽的含量,促进牙周组织修复。3.AS能降低炎症介质IL-1、IL-6、TNF-α的含量,控制炎症对牙槽骨的破坏,缓解DP的组织炎症。
[Abstract]:Objective to study the effect of Astragaloside as on diabetic periodontitis (DP) rats and its correlation with inflammatory factors, and to provide a new drug target and theoretical basis for the clinical treatment of diabetic periodontitis. Methods streptozotocin (STZ) was injected intraperitoneally in combination with high glucose and high fat food to induce diabetes mellitus (DM) rat model. A series of chronic complications occurred after high glucose and high fat feeding for 12 weeks. On this basis, the maxillary molars were inoculated with Porphyromonas gingivalis combined with ligation of filaments to induce periodontitis, and the treatment group was given as intragastric perfusion. Control group (group N), group DP (group DP), high as group (group AS-H) and low dose group (group AS-L) were set up. Blood glucose concentration, gingival inflammation and periodontal bag depth were compared, and the changes of glycosylation end products ages, glycosylated hemoglobin (glycosylated hemoglobin) HbA1C levels were detected. Enzyme linked immunosorbent assay (Elisa) was used to detect the changes of inflammatory factors in the periodontal mucosa of rats in four groups, including the expression of IL-1 and IL-6, TNF- 伪, and the relationship between as and inflammatory factors. Result 1. The changes of blood glucose and urine sugar: the fasting blood glucose of the successful DM rats was higher than 16.7 mmol / L, which was significantly higher than that of the N group (p0.05). In the high and low dose groups treated with as, blood glucose decreased, urine glucose was negative or weakly positive, the difference was statistically significant (p0.05). Periodontal examination: in as group, the gingival inflammation was alleviated, the periodontal bag was shallower, the severe gingival inflammation was found in DP group, the periodontal pouch was detected more deeply, and the level of attachment loss was higher than that in N group. Pairwise comparison has statistical significance (p0.05). The paraffin sections of periodontal mucosa in the affected side of rats were stained with HE to show the infiltration of a large number of inflammatory cells in the periodontal pocket of DP group. The alveolar bone showed horizontal absorption in the maxillary bone in vitro. As treatment group showed that the degree of inflammatory cell infiltration in periodontal pouch was decreased and no such changes were found in the N group. 4. The levels of ages and HB A1C in as treatment group were lower than those in DP group by biochemical analyzer. The levels of ages and HB A1C in DP group were higher than those in DP group, and the level of serum C peptide in N group was at normal level, and the difference was statistically significant (p0.05) .5. Enzyme linked immunosorbent assay (Elisa) was used to detect the level of IL-1-, IL-6-, TNF- 伪 in as treated group, which was significantly lower than that in DP group (p0.05). Conclusion 1. As has the function of hypoglycemia. 2. As can decrease the level of AGEsHb A1C, increase the content of C-peptide, promote periodontal tissue repair. 3.AS can decrease the content of IL-1TNF- 伪 and control the destruction of alveolar bone caused by inflammation. Relieve the inflammation of the tissue of DP.
【学位授予单位】：锦州医科大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R781.4;R587.2

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