Fat-1转基因小鼠对肥胖的抵抗作用的研究

发布时间：2018-07-07 18:42

  本文选题：肥胖 + n-3多不饱和脂肪酸　； 参考：《青岛大学》2015年硕士论文

【摘要】：目的研究fat-1转基因小鼠增加内源性n-3多不饱和脂肪酸(Polyunsaturated fat acids,PUFAs)对小鼠肥胖的抵抗作用,从体重、血清中血脂及胃组织中肥胖相关因子nesfatin-1和ghrelin的变化,探讨其作用机理。方法将小鼠分为高脂饮食fat-1转基因组和非转基因组及正常饮食fat-1转基因组和非转基因组4个组。每周测量各组小鼠体质量和体长,实验结束时处死四组小鼠,称量小鼠内脏周围脂肪质量;取血测定各组小鼠血清中血糖(FBG)、甘油三酯(TG)、总胆固醇(TC)、高密度脂蛋白胆固醇(HDL-C)、低密度脂蛋白胆固醇(LDL-C)含量;测定胃组织内nesfatin-1、ghrelin m RNA的相对表达量和蛋白表达量及其与Lee's指数的相关性。结果转基因小鼠的内脏周围脂肪质量均低于非转基因小鼠(F=20.56~41.75,P0.01);转基因小鼠血清FBG、TG、TC、HDL-C、LDL-C水平均低于非转基因小鼠(F=15.59~94.07,P0.01);高脂饮食使nesfatin-1 m RNA的相对表达量显著上调(F=25.75,P0.01),并且与Lee's指数成正相关(r=0.621,P0.01),转基因小鼠比非转基因小鼠表达量低并且差异显著(q=7.035,6.012,P0.05)。高脂饮食使ghrelin m RNA的相对表达量显著下调(F=28.42,P0.01),且与Lee's指数成负相关(r=-0.603,P0.01),转基因小鼠均比非转基因小鼠表达量高,并且差异具有统计学意义(q=4.112,3.379,P0.05)。结论Fat-1转基因小鼠体内含有的n-3多不饱和脂肪酸脱氢酶增加了内源性n-3PUFA,从而降低体内n-6/n-3比值,降低了小鼠体重,调节了血脂及肥胖因子的表达,改善机体能量代谢,预防了肥胖的发生。
[Abstract]:Objective to study the effects of increasing endogenous n-3 fat polyunsaturated fatty acids (PUFAs) on obesity in fat-1 transgenic mice, and to explore its mechanism from the changes of body weight, serum lipids and nesfatin-1 and ghrelin in gastric tissues. Methods mice were divided into four groups: high fat diet fat-1 transgenic group and non-transgenic group, normal diet fat-1 transgenic group and non-transgenic group. The body weight and body length of each group were measured every week. At the end of the experiment, the four groups of mice were killed to weigh the weight of fat around the viscera of the mice. Serum glucose (FBG), triglyceride (TG), total cholesterol (TC), high density lipoprotein cholesterol (HDL-C) and low density lipoprotein cholesterol (LDL-C) were measured. The relative expression and protein expression of nesfatin-1 hghrelin mRNA in gastric tissue and its correlation with Leehs index were measured. Results the quality of visceral fat in transgenic mice was significantly lower than that in non-transgenic mice (FB20.56A 41.75U P0.01), the serum levels of FBGG + TGG + TGG (HDL-CU LDL-C) in transgenic mice were lower than those in non-transgenic mice (F15.5994.07g / P0.01), and the relative expression of nesfatin-1 mRNA was significantly up-regulated by high-fat diet (FG 25.75U P0.01), and the relative expression of nesfatin-1 mRNA was significantly higher than that of non-transgenic mice (FF25.75P0.01), and the level of LDL-C in serum of transgenic mice was lower than that of non-transgenic mice. The expression level of transgenic mice was significantly lower than that of non-transgenic mice (P 0.05). The relative expression of ghrelin mRNA was significantly down-regulated by high-fat diet (F _ (28.42) P _ (0.01), and negatively correlated with the Leehs index (r ~ (-0.603) P _ (0.01). The expression of ghrelin mRNA in transgenic mice was significantly higher than that in non-transgenic mice (P < 0.05). Conclusion n-3 polyunsaturated fatty acid dehydrogenase in Fat-1 transgenic mice can increase endogenous n-3PUFAs, decrease the ratio of n-6/n-3 in vivo, decrease the body weight, regulate the expression of lipid and obesity factors, and improve energy metabolism. To prevent obesity.
【学位授予单位】：青岛大学
【学位级别】：硕士
【学位授予年份】：2015
【分类号】：R589.2

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中国期刊全文数据库 前1条

1 徐姝迪;郑玉建;丁红;姬凤彩;王忠;艾力·吾布里卡斯木;任美玲;;高脂饲料诱导肥胖大鼠模型的方法研究[J];现代预防医学;2012年02期

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