Let-7a通过调控骨髓间充质干细胞增殖影响骨质疏松的研究

发布时间：2018-07-08 19:05

本文选题：骨髓间充质干细胞 + 增殖　；参考：《上海交通大学学报(医学版)》2017年04期

【摘要】：目的·研究雌激素缺乏所致骨质疏松发生过程中Let-7a对骨髓间充质干细胞(BMSC)增殖能力的影响。方法·取20只8周龄健康雌性小鼠,行双侧卵巢切除术(OVX),建立雌激素缺乏骨质疏松动物模型。另取20只健康雌性小鼠行卵巢附近脂肪组织部分切除术(sham)。术后2个月,Micro-CT检测2组小鼠股骨干骺端骨密度、骨体积分数及骨小梁数量;MTT检测2组BMSC的增殖能力;real-time PCR检测2组BMSC中Let-7a的表达;上调或下调BMSC内Let-7a的表达水平后,MTT检测BMSC的增殖能力。结果·Micro-CT显示OVX组股骨干骺端骨密度、骨体积分数及骨小梁数量均低于sham组。成脂能力及成骨能力检测发现,OVX组BMSC成骨能力低于sham组,成脂能力高于sham组。MTT显示OVX组BMSC增殖能力低于sham组。OVX组BMSC中Let-7a的表达明显高于sham组。上调Let-7a的表达水平后,BMSC增殖水平下降;下调Let-7a的表达水平后,BMSC增殖水平上升。结论·在雌激素缺乏所致的骨质疏松小鼠中,高表达的Let-7a抑制BMSC增殖,可能是导致骨质疏松发生的原因之一。
[Abstract]:Objective to study the effect of Let-7a on the proliferation of bone marrow mesenchymal stem cells (BMSC) during osteoporosis induced by estrogen deficiency. Methods OVX was performed on 20 8 week old healthy female mice to establish osteoporosis model. Another 20 healthy female mice were selected for partial excision of adipose tissue near ovary (sham). Bone mineral density (BMD), bone volume fraction (BV) and the number of trabeculae were measured by Micro-CT 2 months after operation. The proliferative ability of BMSC in both groups was detected by real-time PCR and the expression of Let-7a in BMSC was detected by real-time PCR. After up-regulating or down-regulating the expression of Let-7a in BMSC, MTT assay was used to detect the proliferative ability of BMSC. Results Micro-CT showed that bone mineral density (BMD), bone volume fraction (BV) and the number of trabeculae in OVX group were lower than those in sham group. The expression of Let-7a in OVX group was significantly higher than that in sham group (P < 0.05), but higher in sham group than in sham group (P < 0.05), and the expression of Let-7a in OVX group was significantly higher than that in sham group (P < 0.05), and the expression of Let-7a in OVX group was significantly higher than that in sham group (P < 0.05). The proliferation level of BMSC decreased after up-regulating the expression level of Let-7a, and the proliferation level of BMSC increased after down-regulating the expression level of Let-7a. Conclusion the high expression of Let-7a inhibits the proliferation of BMSC in osteoporosis mice induced by estrogen deficiency, which may be one of the causes of osteoporosis.
【作者单位】：重庆医科大学附属口腔医院牙体牙髓科口腔疾病与生物医学重庆市重点实验室重庆市高校市级口腔生物医学工程重点实验室;
【基金】：国家自然科学基金(31571508) 重庆市卫生局医学科研计划重点项目(2011-1-062)~~
【分类号】：R580

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