糖尿病患者血浆减少α-突触核蛋白的磷酸化和寡聚化

发布时间：2018-07-11 17:17

本文选题：α-突触核蛋白 + 型糖尿病　；参考：《首都医科大学学报》2017年06期

【摘要】：目的研究2型糖尿病(type 2 diabetes,T2D)患者血浆对α-突触核蛋白(α-synuclein,α-Syn)磷酸化和寡聚化聚集的影响。方法收集首都医科大学宣武医院内分泌科T2D住院患者70例,另收集年龄和性别与之匹配的健康志愿者70例作为对照组。采集抗凝血,并分离血浆。将基因重组人α-Syn在患者和对照志愿者血浆中振荡孵育,ELISA法检测各组人群血浆中寡聚化和磷酸化α-Syn形成量。结果 T2D患者血浆中寡聚化和磷酸化α-Syn形成量较对照组均有显著降低(P0.05),且其与年龄有相关性。结论 T2D患者血浆以年龄依赖的方式减少寡聚化和磷酸化α-Syn的形成。
[Abstract]:Objective to study the effect of plasma on 伪 -synuclein (伪 -Syn) phosphorylation and oligomerization in patients with type 2 diabetes mellitus (type 2 diabetic T2D). Methods 70 T2D inpatients from Department of Endocrinology, Xuanwu Hospital, Capital Medical University, and 70 healthy volunteers matched by age and sex were collected as control group. Anticoagulant blood was collected and plasma was isolated. Oligomerization and phosphorylation of 伪 -Syn in the plasma of patients and control volunteers were detected by Elisa. Results the plasma oligomerization and phosphorylation of 伪 -Syn in T2D patients were significantly lower than those in the control group (P0.05), and there was a correlation between the formation of 伪 -Syn and age. Conclusion the plasma of T 2 D patients reduces the formation of oligomerization and phosphorylation of 伪-Syn in an age-dependent manner.
【作者单位】：首都医科大学宣武医院神经生物学研究室北京市老年病医疗研究中心;首都医科大学帕金森病临床诊疗与研究中心;帕金森病北京市重点实验室和教育部神经变性病重点实验室;国家老年疾病临床医学研究中心;
【基金】：国家自然科学基金(81371200,81071014,81401042) 北京市医院管理局“使命”计划专项经费资助(SML20150803) 北京市科学技术委员会资助(Z161100005116011,Z171100000117013) 北京市卫生和计划生育委员会“老年重大疾病关键技术研究”(PXM2017_026283_000002)~~
【分类号】：R587.1

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