可溶性环氧化物水解酶抑制剂的降血糖功效研究

发布时间：2018-07-12 16:01

本文选题：Ⅱ型糖尿病 + 可溶性环氧化物水解酶抑制剂　；参考：《中国畜牧兽医》2017年11期

【摘要】：为探究可溶性环氧化物水解酶抑制剂(soluble epoxide hydrolase inhibitors,sEHI)的降血糖功效,试验以高脂饲料与链脲佐菌素(streptozotocin,STZ)联合诱导的Ⅱ型糖尿病小鼠为研究对象,将C57BL/6J小鼠随机分为:正常对照组、糖尿病模型组和sEHI组,sEHI组饲喂高脂饲料,每日灌胃sEHI(t-AUCB)1 mg/kg,连续灌胃4周;糖尿病模型组饲喂高脂饲料,每日灌胃同等计量的生理盐水;正常对照组饲喂普通饲料,每日灌胃同等体积的生理盐水。期间各组小鼠均自由采食及饮水,记录小鼠体重、血糖并进行口服葡萄糖耐量试验。试验结束后,测定血清血脂指标、肝脏过氧化指标并对小鼠胰岛组织进行病理学分析。结果显示,与糖尿病模型组相比,采用sEHI干预后糖尿病小鼠的空腹血糖显著降低(P0.05),而葡萄糖耐量水平升高;小鼠血清总胆固醇(TC)、甘油三酯(TG)、低密度脂蛋白(LDL)水平明显降低,其中TC、TG水平与糖尿病模型组相比差异显著(P0.05),而高密度脂蛋白与胆固醇比值(HDL/TC)水平则显著升高(P0.05);与糖尿病模型组相比,sEHI干预组小鼠肝脏的超氧化物歧化酶(SOD)、过氧化氢酶(CAT)活性显著提高(P0.05),丙二醛(MDA)活性显著降低(P0.05);小鼠的胰岛组织切片分析结果表明,sEHI对小鼠胰岛组织有明显的修复作用。综上所述,sEHI具有降血糖、减轻自由基损伤、抑制脂质过氧化、修复受损胰岛组织的作用。
[Abstract]:In order to investigate the hypoglycemic effect of soluble epoxide hydrolase inhibitor (soluble epoxide hydrolase inhibitors EHI, C57BL / 6J mice were randomly divided into two groups: normal control group. The diabetic model group and the sEHI group were fed with high fat diet and sEHI (t-AUCB) 1 mg / kg daily for 4 weeks; the diabetic model group was fed with the same amount of physiological saline daily; the normal control group was fed with normal diet. Intragastric administration of the same volume of saline daily. During the period, the mice were fed and drank freely. The body weight, blood glucose and oral glucose tolerance test were recorded. At the end of the experiment, serum lipid index and liver peroxidation index were measured and the islet tissues of mice were analyzed by pathology. The results showed that compared with the diabetic model group, the fasting blood glucose decreased significantly (P0.05), the glucose tolerance increased, the serum total cholesterol (TC), triglyceride (TG), low density lipoprotein (LDL) levels decreased significantly in the diabetic mice treated with sEHI, and the levels of serum total cholesterol (TC), triglyceride (TG), low density lipoprotein (LDL) were significantly decreased in the diabetic mice. Compared with diabetic model group, the level of TCU TG was significantly higher than that of diabetic model group (P0.05), while the HDL / TC ratio was significantly increased (P0.05), and the liver superoxide dismutase (SOD) of mice treated with EHI was significantly higher than that of diabetic model group (P0.05), and the level of liver superoxide dismutase (SOD) was significantly higher than that of diabetic model group (P0.05). The activity of catalase (cat) increased significantly (P0.05) and the activity of malondialdehyde (MDA) decreased significantly (P0.05). In conclusion, EHI can reduce blood glucose, reduce free radical damage, inhibit lipid peroxidation and repair damaged islet tissue.
【作者单位】：内蒙古农业大学乳品生物技术与工程教育部重点实验室;内蒙古骆驼研究院;
【基金】：国家自然科学基金项目(31360397) 国际科技合作项目(2015DFR30680、ky201401002) 内蒙古重大专项
【分类号】：R587.1

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