肥胖大鼠体内钙代谢状态的变化及钙与肥胖关系的研究

发布时间：2018-07-15 07:18

【摘要】：目的:研究肥胖对大鼠钙代谢状态的改变以及补钙对肥胖产生的作用,为进一步探讨肥胖与钙之间的相互关系提供理论依据。方法:按随机分组原则,40只雄性Wistar大鼠被分为对照组10只和肥胖组30只,分别喂以普通饲料和高脂饲料,建立肥胖大鼠模型,8周后将对照组的10只大鼠和肥胖组中随机选取的10只大鼠处死。取血清检测如下指标:血清钙、碱性磷酸酶、甲状旁腺激素、降钙素、维生素D受体、钙结合蛋白、25羟基维生素D。取小肠组织采用实时定量PCR方法检测维生素D受体、钙结合蛋白基因的表达。用剩余的20只肥胖大鼠继续进行第二部分实验。将20只肥胖大鼠随机分为肥胖对照组10只和补钙组10只,均喂以普通饲料,每天定时定量对补钙组用葡萄糖酸钙溶液灌胃,同时对肥胖对照组灌以等量生理盐水,15天后全部处死。取血清检测如下指标:血脂四项、骨钙素、25羟基维生素D。取皮下白色脂肪组织采用实时定量PCR方法检测瘦素、脂肪酸合成酶基因的表达。结果:1、与对照组比较,肥胖组大鼠血清钙水平较低,碱性磷酸酶活力较高,且经检验差异均具有统计学意义(P0.05)。2、与对照组比较,肥胖组大鼠血清甲状旁腺激素水平较高,降钙素水平较低,且经检验差异均具有统计学意义(P0.05);肥胖组大鼠血清钙结合蛋白水平较低,维生素D受体水平较高,25羟基维生素D水平较低,且经检验差异均具有统计学意义(P0.05)。3、与对照组比较,肥胖组大鼠小肠组织中钙结合蛋白基因表达量和维生素D受体基因表达量均较低,且经检验差异均具有统计学意义(P0.05)。4、与肥胖对照组比较,补钙组大鼠体重、Lee’s指数均较低,且经检验差异均具有统计学意义(P0.05);补钙组大鼠总胆固醇、甘油三酯、低密度脂蛋白水平均较低,且经检验差异均具有统计学意义(P0.05),高密度脂蛋白水平较高,且经检验差异具有统计学意义(P0.05)。5、与肥胖对照组比较,补钙组大鼠血清骨钙素、25羟基维生素D水平均较高,且经检验差异均具有统计学意义(P0.05)。6、与肥胖对照组比较,补钙组大鼠白色脂肪组织瘦素基因、脂肪酸合成酶基因表达量均较低,且经检验差异均具有统计学意义(P0.05)。结论:1、肥胖会引起大鼠血清钙水平降低,碱性磷酸酶活力升高。2、肥胖会引起大鼠血清甲状旁腺激素水平升高,降钙素水平降低,钙结合蛋白水平降低,维生素D受体水平降低,25羟基维生素D水平降低。3、肥胖会使大鼠小肠组织中钙结合蛋白基因表达量和维生素D受体基因表达量降低。4、补钙会使大鼠体重、Lee’s指数降低,使大鼠总胆固醇、甘油三酯、低密度脂蛋白水平降低,高密度脂蛋白水平升高。5、补钙会使大鼠血清骨钙素、25羟基维生素D水平升高。6、补钙会使大鼠白色脂肪组织瘦素基因、脂肪酸合成酶基因表达量降低。
[Abstract]:Aim: to study the changes of calcium metabolism in rats with obesity and the effect of calcium supplementation on obesity so as to provide a theoretical basis for the further study of the relationship between obesity and calcium. Methods: forty male Wistar rats were randomly divided into control group (n = 10) and obese group (n = 30). After 8 weeks, 10 rats in the control group and 10 rats in the obese group were killed. Serum calcium, alkaline phosphatase, parathyroid hormone, calcitonin, vitamin D receptor, calcium binding protein 25 hydroxyvitamin D. The expression of vitamin D receptor and calcium binding protein gene was detected by real-time quantitative PCR in small intestine. The second part of the experiment was continued with the remaining 20 obese rats. Twenty obese rats were randomly divided into obese control group (n = 10) and calcium supplement group (n = 10). At the same time, the obese control group was given the same amount of normal saline for 15 days. The serum samples were collected to detect the following indexes: blood lipids four items, osteocalcin 25 hydroxyvitamin D. The expression of leptin and fatty acid synthase gene was detected by real-time quantitative PCR in subcutaneous white adipose tissue. Results compared with the control group, the serum calcium level and alkaline phosphatase activity of obese rats were lower, and the differences were statistically significant (P0.05) .2.Compared with the control group, the serum parathyroid hormone levels of obese rats were higher. Calcitonin level was lower, and the difference was statistically significant (P0.05), the serum calcium binding protein level was lower, the vitamin D receptor level was higher and the vitamin D level was lower in obesity group. Compared with the control group, the expression of calcium binding protein gene and vitamin D receptor gene in the small intestine of obese rats were lower than those of the control group. The differences were statistically significant (P0.05) .4Compared with the obese control group, the body weight index of rats in calcium supplementation group was lower, and the difference was statistically significant (P0.05), and the total cholesterol, triglyceride and triglyceride in calcium supplementation group were significantly lower than those in the obese control group (P0.05). The low density lipoprotein level was lower, and the difference was statistically significant (P0.05), the high density lipoprotein level was higher, and the difference was statistically significant (P0.05). The serum levels of osteocalcin 25 hydroxyvitamin D in the calcium supplementation group were higher, and the differences were statistically significant (P0.05) .6. Compared with the obese control group, the leptin gene in the white adipose tissue in the calcium supplementation group was significantly higher than that in the control group. Fatty acid synthase gene expression was low, and the differences were statistically significant (P0.05). Conclusion: weight 1, obesity can decrease serum calcium level, increase alkaline phosphatase activity, increase serum parathyroid hormone level, calcitonin level, calcium binding protein level, and increase serum parathyroid hormone level, calcitonin level and calcium binding protein level. The level of vitamin D receptor decreased. 3. Obesity decreased the expression of calcium binding protein gene and vitamin D receptor gene in the small intestine of rats, and calcium supplementation decreased the Lees index of rat body weight. The levels of total cholesterol, triglyceride, low density lipoprotein, high density lipoprotein, calcium supplementation increased the serum osteocalcin 25 hydroxyl vitamin D level, and calcium supplementation increased the leptin gene in white adipose tissue of rats. The expression of fatty acid synthase gene decreased.
【学位授予单位】：吉林大学
【学位级别】：硕士
【学位授予年份】：2016
【分类号】：R589.2

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