中链脂肪酸诱导的棕色脂肪分化和增殖及其在肥胖控制中的作用
发布时间：2018-07-23 07:41
【摘要】：目的1.观察含6种含中链脂肪酸的食用油对肥胖小鼠体脂肪及血脂的影响。2.观察中链脂肪酸对棕色脂肪剔除的小鼠体脂肪及血脂的影响。3.观察中链脂肪酸对肥胖小鼠棕色脂肪增殖分化的影响。方法1.选取肥胖小鼠105只,随机分为7组,每组15只,分别喂饲不同中链脂肪酸含量的饲料(6种)和大豆油饲料,喂饲16周。观察每组小鼠体质量、体脂肪、Lee氏指数、血脂、肝脏脂肪浸润情况、白色脂肪细胞脂滴大小与大豆油组的差异。2.选取剔除了棕色脂肪的肥胖小鼠30只,随机分为2组,每组15只,分别喂饲MCT饲料和LCT饲料,喂饲12周。观察两组小鼠体质量、体脂肪、血脂、肝脏脂肪浸润情况、白色脂肪细胞脂滴大小以及腹部皮下脂肪中UCP1 mRNA表达的变化。3.选取肥胖小鼠30只,随机分为2组,每组15只,分别喂饲MCT饲料和LCT饲料,喂饲12周。观察两组小鼠体质量、体脂肪、血脂、棕色脂肪micro-PET显像、腹部皮下脂肪及棕色脂肪中 UCP1、BMP7、CIDEA、PRDM16、Cebp-alpha、Cebp-beta、Dio2、Fabp1、Fabp3、 Fabp4、FOXC2、Gyk、PPAR-alpha、PPAR-gamma、PGC1-alpha、PGC1-beta、COX2的mRNA及蛋白表达水平的变化。结果1.干预喂养16周后,6组喂饲含中链脂肪酸食用油的小鼠的体质量、体脂肪量、Lee氏指数、血甘油三酯及总胆固醇浓度均显著低于大豆油对照组(P0.05),肝脏组织的脂肪浸润程度以及白色脂肪细胞的脂滴面积均小于大豆油组(P0.05)。2.剔除了棕色脂肪的肥胖小鼠,喂饲MCT和LCT饲料12周,未观察到两组小鼠体质量、体脂肪、血脂、肝脏脂肪浸润情况及白色脂肪细胞脂滴大小的显著差异(P0.05),但MCT组小鼠腹部皮下脂肪中UCP1 mRNA表达明显高于LCT组(P0.05)。3.干预喂养12周后,MCT组小鼠的体重、体脂肪量及血甘油三酯水平均显著低于LCT组(P0.05), MCT组棕色脂肪重量显著高于LCT组(P0.05)。Micro-PET成像显示MCT组棕色脂肪体积及代谢活性均明显高于LCT组。MCT组棕色脂肪组织中UCP1、CIDEA、Fabp4、PPAR-gamma、PRDM16、BMP7 的 mRNA 及蛋白表达水平均显著高于 LCT 组(P0.05)。MCT 组棕色脂肪组织中 PGC1-alpha、FOXC2、COX2、Dio2、PGC1-beta、PPAR-alpha、CEBP-alpha、CEBP-beta 的 mRNA 表达水平均显著高于 LCT组(P0.05)。MCT 组腹部皮下脂肪中 UCP1、CIDEA、Fabp4、PGC1-alpha、PPAR-gamma、PRDM16、FOXC2、BMP7的mRNA及蛋白表达水平均显著高于LCT组(P0.05)。MCT 组腹部皮下脂肪中 COX2、Fabp1、Fabp3、Dio2、Gyk、PGC1-beta、PPAR-alpha、CEBP-alpha、CEBP-beta 的 mRNA 表达水平均显著高于 LCT 组(P0.05)。结论1.中链脂肪酸的含量在10%～16%之间且辛酸癸酸的比例在2: 1～3: 1之间的食用油可有效降低肥胖小鼠体重、减少体脂蓄积、改善血脂。2.棕色脂肪是中链脂肪酸降脂减重作用的重要靶器官。3.中链脂肪酸可促进棕色脂肪的分化、增殖及功能的增强以及促进白色脂肪的棕色化。
[Abstract]:Objective 1. to observe the effect of 6 kinds of edible oil containing medium chain fatty acids on body fat and blood lipid in obese mice. The effect of chain fatty acid on body fat and blood lipid in mice with brown fat rejection in.2.. The effect of chain fatty acid on the proliferation and differentiation of brown fat in obese mice was observed in.3.. Method 1. 105 obese mice were randomly divided into 7 groups. 15 rats in each group were fed with different medium chain fatty acid content (6 kinds) and soybean oil feed for 16 weeks. The body mass, body fat, Lee's index, blood lipid, liver fatty infiltration, and the difference between the size of white fat cells and the soybean oil group were observed in each group, and 30 obese mice were selected to reject brown fat, and were randomly divided into 2 groups, which were randomly divided into 2 15 rats in each group were fed with MCT feed and LCT feed and fed for 12 weeks. The body mass of the two groups of mice, body fat, blood lipid, liver fatty infiltration, the size of white adipocyte lipid droplets and the change of UCP1 mRNA expression in the abdominal subcutaneous fat were selected, and 30 obese mice were selected and randomly divided into 2 groups, 15 rats in each group were fed with MCT feed and LCT feeding respectively. Material, feed for 12 weeks. Observe the body mass of two groups of mice, body fat, lipid, brown fat micro-PET imaging, abdominal subcutaneous fat and brown fat in UCP1, BMP7, CIDEA, PRDM16, Cebp-alpha, Cebp-beta, Dio2, Fabp1, Fabp3, Fabp4, FOXC2, Fabp3, and protein expression level changes. Results 1. After 16 weeks of intervention feeding, the body mass, body fat volume, Lee's index, triglyceride and total cholesterol in the 6 groups were significantly lower than that of the control group (P0.05), the degree of fatty infiltration of the liver tissue and the area of white fat cells were less than that of the soybean oil group (P0.05).2. eliminated the brown. The obese mice were fed with MCT and LCT for 12 weeks. The body mass of the two groups, body fat, blood lipid, liver fatty infiltration and the size of white fat cells were significantly different (P0.05), but the expression of UCP1 mRNA in the abdominal subcutaneous fat of group MCT mice was significantly higher than that of the LCT group (P0.05).3. intervention for 12 weeks, and MCT mice in the MCT group Weight, body fat and triglyceride were significantly lower than group LCT (P0.05), and the weight of brown fat in group MCT was significantly higher than that in group LCT (P0.05).Micro-PET imaging showed that the volume and metabolic activity of brown fat in group MCT was significantly higher than that in group.MCT in group LCT.MCT UCP1, CIDEA, Fabp4, and protein expression water. The average levels of PGC1-alpha, FOXC2, COX2, Dio2, PGC1-beta, PPAR-alpha, CEBP-alpha, CEBP-beta in the brown adipose tissue of group LCT (P0.05).MCT were significantly higher than those in the abdominal subcutaneous fat. The levels of COX2, Fabp1, Fabp3, Dio2, Gyk, PGC1-beta, PPAR-alpha, CEBP-alpha, in the abdominal subcutaneous fat of group LCT (P0.05).MCT were significantly higher than those in the group. Conclusion the content of fatty acids in the 1. chain is between 10% and 16% and the proportion of octanoic acid decanoic acid between 1 and 1 can be effectively reduced. Weight of fat mice, reducing body fat accumulation, improving blood lipid.2. brown fat is an important target organ for reducing fat and weight loss of medium chain fatty acids,.3. of chain fatty acids can promote the differentiation of brown fat, increase the proliferation and function and promote the browning of white fat.
【学位授予单位】：中国人民解放军医学院
【学位级别】：博士
【学位授予年份】：2017
【分类号】：R589.2
本文编号：2138680
[Abstract]:Objective 1. to observe the effect of 6 kinds of edible oil containing medium chain fatty acids on body fat and blood lipid in obese mice. The effect of chain fatty acid on body fat and blood lipid in mice with brown fat rejection in.2.. The effect of chain fatty acid on the proliferation and differentiation of brown fat in obese mice was observed in.3.. Method 1. 105 obese mice were randomly divided into 7 groups. 15 rats in each group were fed with different medium chain fatty acid content (6 kinds) and soybean oil feed for 16 weeks. The body mass, body fat, Lee's index, blood lipid, liver fatty infiltration, and the difference between the size of white fat cells and the soybean oil group were observed in each group, and 30 obese mice were selected to reject brown fat, and were randomly divided into 2 groups, which were randomly divided into 2 15 rats in each group were fed with MCT feed and LCT feed and fed for 12 weeks. The body mass of the two groups of mice, body fat, blood lipid, liver fatty infiltration, the size of white adipocyte lipid droplets and the change of UCP1 mRNA expression in the abdominal subcutaneous fat were selected, and 30 obese mice were selected and randomly divided into 2 groups, 15 rats in each group were fed with MCT feed and LCT feeding respectively. Material, feed for 12 weeks. Observe the body mass of two groups of mice, body fat, lipid, brown fat micro-PET imaging, abdominal subcutaneous fat and brown fat in UCP1, BMP7, CIDEA, PRDM16, Cebp-alpha, Cebp-beta, Dio2, Fabp1, Fabp3, Fabp4, FOXC2, Fabp3, and protein expression level changes. Results 1. After 16 weeks of intervention feeding, the body mass, body fat volume, Lee's index, triglyceride and total cholesterol in the 6 groups were significantly lower than that of the control group (P0.05), the degree of fatty infiltration of the liver tissue and the area of white fat cells were less than that of the soybean oil group (P0.05).2. eliminated the brown. The obese mice were fed with MCT and LCT for 12 weeks. The body mass of the two groups, body fat, blood lipid, liver fatty infiltration and the size of white fat cells were significantly different (P0.05), but the expression of UCP1 mRNA in the abdominal subcutaneous fat of group MCT mice was significantly higher than that of the LCT group (P0.05).3. intervention for 12 weeks, and MCT mice in the MCT group Weight, body fat and triglyceride were significantly lower than group LCT (P0.05), and the weight of brown fat in group MCT was significantly higher than that in group LCT (P0.05).Micro-PET imaging showed that the volume and metabolic activity of brown fat in group MCT was significantly higher than that in group.MCT in group LCT.MCT UCP1, CIDEA, Fabp4, and protein expression water. The average levels of PGC1-alpha, FOXC2, COX2, Dio2, PGC1-beta, PPAR-alpha, CEBP-alpha, CEBP-beta in the brown adipose tissue of group LCT (P0.05).MCT were significantly higher than those in the abdominal subcutaneous fat. The levels of COX2, Fabp1, Fabp3, Dio2, Gyk, PGC1-beta, PPAR-alpha, CEBP-alpha, in the abdominal subcutaneous fat of group LCT (P0.05).MCT were significantly higher than those in the group. Conclusion the content of fatty acids in the 1. chain is between 10% and 16% and the proportion of octanoic acid decanoic acid between 1 and 1 can be effectively reduced. Weight of fat mice, reducing body fat accumulation, improving blood lipid.2. brown fat is an important target organ for reducing fat and weight loss of medium chain fatty acids,.3. of chain fatty acids can promote the differentiation of brown fat, increase the proliferation and function and promote the browning of white fat.
【学位授予单位】：中国人民解放军医学院
【学位级别】：博士
【学位授予年份】：2017
【分类号】：R589.2
【参考文献】
相关期刊论文 前1条
1 WU LiZhen;ZHOU LinKang;CHEN Cheng;GONG JingYi;XU Li;YE Jing;LI De;LI Peng;;Cidea controls lipid droplet fusion and lipid storage in brown and white adipose tissue[J];Science China(Life Sciences);2014年01期
，本文编号：2138680
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