硅油对淀粉样蛋白和多肽积聚的促进作用
发布时间：2018-07-29 14:21
【摘要】：淀粉样疾病吸引了无数科学家的兴趣，被Igor K. Lednev称为世界第八大奇迹。其中，疏水界面对淀粉样蛋白和多肽积聚的影响，自1994年起就陆续有文章报道。蛋白单体不仅富集在疏水界面上，并且在界面上采取有助于积聚的构象和排列，甚至宏观气液界面也可显著促进α-突触核蛋白（α-Syn）的积聚。 硅油是一类聚硅氧烷，呈疏水性，广泛应用于工业、医疗和日常用品中，通常被认为是对人体安全无害的。例如，在眼科手术和美容整形中，常使用硅油作为填充物。水中的硅油微粒表面是一种疏水界面，但硅油对淀粉样蛋白和多肽积聚的影响，尚未有报道。 本文采用硫磺素Ｔ(ThT)荧光法和原子力显微镜表征，研究了硅油对AcP11肽、α-突触核蛋白(α-Syn)、淀粉样蛋白β1-42(Human Aβ42)、酵母类Prion蛋白Ure2蛋白和GAV9肽的影响，，结果显示，五种淀粉样蛋白/多肽在含有硅油的体系中积聚更快、更充分，表明一定量的硅油能促进这些淀粉样蛋白/多肽的积聚。 此外，在常规的生物实验中，会使用离心管等容器，而且多数容器在制作时，会对内表面进行硅油处理。本实验研究了15ml离心管在涡旋操作时，对硅油污染的引入。结果表明，涡旋能将离心管内壁的硅油引入溶液中，随后促进GAV9肽的积聚。 展望方面，调研了眼科手术中使用硅油填充后的并发症，以白内障较为突出，目前发病机制尚不清楚，在临床归纳的可能因素中，并没有提及硅油促进淀粉样蛋白积聚这一因素；而白内障的发生，与晶状体蛋白(Crystallin)的积聚密切相关，可以推测，填充后的白内障并发症，由硅油诱导促进引起。
[Abstract]:Amyloid disease has attracted the interest of countless scientists, Igor K. Lednev called the world's eighth miracle. The effect of hydrophobic interface on the accumulation of amyloid and polypeptide has been reported since 1994. The protein monomers were not only concentrated at the hydrophobic interface, but also conformed and arranged in favor of accumulation at the interface. Even the macro-gas-liquid interface could significantly promote the accumulation of 伪 -synaptic nucleoprotein (伪 -Syn). Silicone oil is a kind of polysiloxane, which is hydrophobic and widely used in industrial, medical and daily necessities. For example, silicone oil is often used as a filler in eye surgery and cosmetic surgery. The surface of silicon oil particles in water is a hydrophobic interface, but the effect of silicone oil on amyloid and polypeptide accumulation has not been reported. The effects of silicone oil on AcP11 peptide, 伪 -syn, amyloid 尾 1-42 (Human A 尾 42, yeast Prion protein Ure2 protein and GAV9 peptide were studied by T (ThT) fluorescence and atomic force microscopy. The accumulation of five kinds of amyloid protein / polypeptide in the system containing silicone oil is faster and more sufficient, which indicates that a certain amount of silicone oil can promote the accumulation of these amyloid proteins / polypeptides. In addition, in conventional biological experiments, centrifuge tubes and other containers are used, and most containers are made with silicone oil on the inner surface. In this paper, the introduction of silicon oil pollution by 15ml centrifuge tube in vortex operation was studied. The results show that the vortex can introduce silicon oil from the inner wall of the centrifuge tube into the solution and then promote the accumulation of GAV9 peptide. In the aspect of prospect, the complications after silicone oil filling in ophthalmic surgery were investigated. Cataract is more prominent. At present, the pathogenesis of cataract is not clear, among the possible factors in clinical induction, However, the occurrence of cataract is closely related to the accumulation of crystallin (Crystallin). It can be inferred that the complications of cataract after filling are induced by silicone oil and promoted by silicone oil.
【学位授予单位】：中国科学院研究生院（上海应用物理研究所）
【学位级别】：硕士
【学位授予年份】：2015
【分类号】：R597.2
本文编号：2152956
[Abstract]:Amyloid disease has attracted the interest of countless scientists, Igor K. Lednev called the world's eighth miracle. The effect of hydrophobic interface on the accumulation of amyloid and polypeptide has been reported since 1994. The protein monomers were not only concentrated at the hydrophobic interface, but also conformed and arranged in favor of accumulation at the interface. Even the macro-gas-liquid interface could significantly promote the accumulation of 伪 -synaptic nucleoprotein (伪 -Syn). Silicone oil is a kind of polysiloxane, which is hydrophobic and widely used in industrial, medical and daily necessities. For example, silicone oil is often used as a filler in eye surgery and cosmetic surgery. The surface of silicon oil particles in water is a hydrophobic interface, but the effect of silicone oil on amyloid and polypeptide accumulation has not been reported. The effects of silicone oil on AcP11 peptide, 伪 -syn, amyloid 尾 1-42 (Human A 尾 42, yeast Prion protein Ure2 protein and GAV9 peptide were studied by T (ThT) fluorescence and atomic force microscopy. The accumulation of five kinds of amyloid protein / polypeptide in the system containing silicone oil is faster and more sufficient, which indicates that a certain amount of silicone oil can promote the accumulation of these amyloid proteins / polypeptides. In addition, in conventional biological experiments, centrifuge tubes and other containers are used, and most containers are made with silicone oil on the inner surface. In this paper, the introduction of silicon oil pollution by 15ml centrifuge tube in vortex operation was studied. The results show that the vortex can introduce silicon oil from the inner wall of the centrifuge tube into the solution and then promote the accumulation of GAV9 peptide. In the aspect of prospect, the complications after silicone oil filling in ophthalmic surgery were investigated. Cataract is more prominent. At present, the pathogenesis of cataract is not clear, among the possible factors in clinical induction, However, the occurrence of cataract is closely related to the accumulation of crystallin (Crystallin). It can be inferred that the complications of cataract after filling are induced by silicone oil and promoted by silicone oil.
【学位授予单位】：中国科学院研究生院（上海应用物理研究所）
【学位级别】：硕士
【学位授予年份】：2015
【分类号】：R597.2
【参考文献】
相关期刊论文 前3条
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2 刘宇英,何曙云,吴剑辉,黄丽源,冼幕慈,邵建勇,赵美卿;一次性注射器所用硅油对家兔肺损伤的实验研究[J];南方护理学报;2001年04期
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本文编号：2152956
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